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Article: Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
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TitleExpression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
 
AuthorsWang, W3
Wei, L3
Yang, A3
He, T3
Yuen, KY2
Chen, C3
Rao, Z1 3
 
Issue Date2009
 
PublisherInternational Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091
 
CitationActa Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528 [How to Cite?]
DOI: http://dx.doi.org/10.1107/S1744309109014055
 
AbstractHuman coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.
 
ISSN1744-3091
2013 Impact Factor: 0.568
2013 SCImago Journal Rankings: 0.552
 
DOIhttp://dx.doi.org/10.1107/S1744309109014055
 
PubMed Central IDPMC2675602
 
ISI Accession Number IDWOS:000265639600025
 
DC FieldValue
dc.contributor.authorWang, W
 
dc.contributor.authorWei, L
 
dc.contributor.authorYang, A
 
dc.contributor.authorHe, T
 
dc.contributor.authorYuen, KY
 
dc.contributor.authorChen, C
 
dc.contributor.authorRao, Z
 
dc.date.accessioned2010-05-31T03:49:20Z
 
dc.date.available2010-05-31T03:49:20Z
 
dc.date.issued2009
 
dc.description.abstractHuman coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationActa Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528 [How to Cite?]
DOI: http://dx.doi.org/10.1107/S1744309109014055
 
dc.identifier.citeulike4441790
 
dc.identifier.doihttp://dx.doi.org/10.1107/S1744309109014055
 
dc.identifier.epage528
 
dc.identifier.hkuros167084
 
dc.identifier.isiWOS:000265639600025
 
dc.identifier.issn1744-3091
2013 Impact Factor: 0.568
2013 SCImago Journal Rankings: 0.552
 
dc.identifier.issue5
 
dc.identifier.pmcidPMC2675602
 
dc.identifier.pmid19407394
 
dc.identifier.scopuseid_2-s2.0-67249102368
 
dc.identifier.spage526
 
dc.identifier.urihttp://hdl.handle.net/10722/59400
 
dc.identifier.volume65
 
dc.languageeng
 
dc.publisherInternational Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091
 
dc.publisher.placeUnited States
 
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications Online
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCrystallography, X-Ray
 
dc.subject.meshGene Expression
 
dc.subject.meshRNA-Binding Proteins - chemistry - genetics - metabolism
 
dc.subject.meshSARS Virus - chemistry - genetics - metabolism
 
dc.subject.meshViral Proteins - chemistry - genetics - metabolism
 
dc.titleExpression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
 
dc.typeArticle
 
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<contributor.author>Yang, A</contributor.author>
<contributor.author>He, T</contributor.author>
<contributor.author>Yuen, KY</contributor.author>
<contributor.author>Chen, C</contributor.author>
<contributor.author>Rao, Z</contributor.author>
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Author Affiliations
  1. Institute of Biophysics Chinese Academy of Sciences
  2. South China Agricultural University
  3. Tsinghua University