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Article: CTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults

TitleCTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults
Authors
Issue Date2008
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/722895/description#description
Citation
Journal Of Microbiology, Immunology And Infection, 2008, v. 41 n. 5, p. 428-432 How to Cite?
AbstractWe investigated the occurrence and diversity of extended-spectrum beta-lactamase (ESBL) enzymes among antibiotic-resistant Escherichia coli and Klebsiella pneumoniae isolates obtained from human feces. All ESBL-positive isolates were characterized at the molecular level by polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (PFGE). Eight of 46 antibiotic-resistant E. coli (6 from children and 2 from adults) and 4 of 8 K. pneumoniae (all from adults) isolates were found to be ESBL-positive by the double-disk synergy test. Seven isolates were found to have CTX-M-14, 2 each had CTX-M-24 and CTX-M-38, and 1 had CTX-M-9. In addition, 8 isolates were found to carry TEM-1b or TEM-1c. No SHV-type enzyme was found among the E. coli strains. In 9 strains, the plasmidic bla CTX-M determinants were transferable to E. coli by conjugation. Analysis by PFGE showed evidence of clonal and non-clonal spread. The present study shows fecal carriage of organisms producing bla CTX-M determinants and underscores the role that commensals could play as a reservoir for their dissemination. © 2008 Journal of Microbiology, Immunology and Infection.
Persistent Identifierhttp://hdl.handle.net/10722/59397
ISSN
2015 Impact Factor: 2.955
2015 SCImago Journal Rankings: 0.789
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorWong, RCWen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorYip, Ken_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorQue, TLen_HK
dc.date.accessioned2010-05-31T03:49:16Z-
dc.date.available2010-05-31T03:49:16Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Microbiology, Immunology And Infection, 2008, v. 41 n. 5, p. 428-432en_HK
dc.identifier.issn1684-1182en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59397-
dc.description.abstractWe investigated the occurrence and diversity of extended-spectrum beta-lactamase (ESBL) enzymes among antibiotic-resistant Escherichia coli and Klebsiella pneumoniae isolates obtained from human feces. All ESBL-positive isolates were characterized at the molecular level by polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (PFGE). Eight of 46 antibiotic-resistant E. coli (6 from children and 2 from adults) and 4 of 8 K. pneumoniae (all from adults) isolates were found to be ESBL-positive by the double-disk synergy test. Seven isolates were found to have CTX-M-14, 2 each had CTX-M-24 and CTX-M-38, and 1 had CTX-M-9. In addition, 8 isolates were found to carry TEM-1b or TEM-1c. No SHV-type enzyme was found among the E. coli strains. In 9 strains, the plasmidic bla CTX-M determinants were transferable to E. coli by conjugation. Analysis by PFGE showed evidence of clonal and non-clonal spread. The present study shows fecal carriage of organisms producing bla CTX-M determinants and underscores the role that commensals could play as a reservoir for their dissemination. © 2008 Journal of Microbiology, Immunology and Infection.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/722895/description#descriptionen_HK
dc.relation.ispartofJournal of Microbiology, Immunology and Infectionen_HK
dc.subject.meshCephalosporin Resistance - geneticsen_HK
dc.subject.meshCephalosporinase - geneticsen_HK
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden_HK
dc.subject.meshEscherichia coli - enzymology - genetics - isolation & purificationen_HK
dc.subject.meshFeces - microbiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKlebsiella pneumoniae - enzymology - genetics - isolation & purificationen_HK
dc.subject.meshMicrobial Sensitivity Testsen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshbeta-Lactam Resistance - geneticsen_HK
dc.subject.meshbeta-Lactamases - geneticsen_HK
dc.titleCTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adultsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1684-1182&volume=41&spage=428&epage=32&date=2008&atitle=CTX-M+type+beta-lactamases+among+fecal+Escherichia+coli+and+Klebsiella+pneumoniae+isolates+in+non-hospitalized+children+and+adults.en_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH:khchowb@hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid19122926-
dc.identifier.scopuseid_2-s2.0-60249100864en_HK
dc.identifier.hkuros165738en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60249100864&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume41en_HK
dc.identifier.issue5en_HK
dc.identifier.spage428en_HK
dc.identifier.epage432en_HK
dc.identifier.isiWOS:000265237900011-
dc.publisher.placeHong Kongen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridWong, RCW=8612000100en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridYip, K=36725059400en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridQue, TL=7003786628en_HK

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