Article: CTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults

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TitleCTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults
AuthorsHo, PL2
Wong, RCW1
Chow, KH1
Yip, K2
Wong, SSY2
Que, TL1
Issue Date2008
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/722895/description#description
CitationJournal Of Microbiology, Immunology And Infection, 2008, v. 41 n. 5, p. 428-432 [How to Cite?]
AbstractWe investigated the occurrence and diversity of extended-spectrum beta-lactamase (ESBL) enzymes among antibiotic-resistant Escherichia coli and Klebsiella pneumoniae isolates obtained from human feces. All ESBL-positive isolates were characterized at the molecular level by polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (PFGE). Eight of 46 antibiotic-resistant E. coli (6 from children and 2 from adults) and 4 of 8 K. pneumoniae (all from adults) isolates were found to be ESBL-positive by the double-disk synergy test. Seven isolates were found to have CTX-M-14, 2 each had CTX-M-24 and CTX-M-38, and 1 had CTX-M-9. In addition, 8 isolates were found to carry TEM-1b or TEM-1c. No SHV-type enzyme was found among the E. coli strains. In 9 strains, the plasmidic bla CTX-M determinants were transferable to E. coli by conjugation. Analysis by PFGE showed evidence of clonal and non-clonal spread. The present study shows fecal carriage of organisms producing bla CTX-M determinants and underscores the role that commensals could play as a reservoir for their dissemination. © 2008 Journal of Microbiology, Immunology and Infection.
ISSN1684-1182
2011 Impact Factor: 0.987
2011 SCImago Journal Rankings: 0.101
ISI Accession Number IDWOS:000265237900011
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHo, PL
dc.contributor.authorWong, RCW
dc.contributor.authorChow, KH
dc.contributor.authorYip, K
dc.contributor.authorWong, SSY
dc.contributor.authorQue, TL
dc.date.accessioned2010-05-31T03:49:16Z
dc.date.available2010-05-31T03:49:16Z
dc.date.issued2008
dc.description.abstractWe investigated the occurrence and diversity of extended-spectrum beta-lactamase (ESBL) enzymes among antibiotic-resistant Escherichia coli and Klebsiella pneumoniae isolates obtained from human feces. All ESBL-positive isolates were characterized at the molecular level by polymerase chain reaction, sequencing and pulsed-field gel electrophoresis (PFGE). Eight of 46 antibiotic-resistant E. coli (6 from children and 2 from adults) and 4 of 8 K. pneumoniae (all from adults) isolates were found to be ESBL-positive by the double-disk synergy test. Seven isolates were found to have CTX-M-14, 2 each had CTX-M-24 and CTX-M-38, and 1 had CTX-M-9. In addition, 8 isolates were found to carry TEM-1b or TEM-1c. No SHV-type enzyme was found among the E. coli strains. In 9 strains, the plasmidic bla CTX-M determinants were transferable to E. coli by conjugation. Analysis by PFGE showed evidence of clonal and non-clonal spread. The present study shows fecal carriage of organisms producing bla CTX-M determinants and underscores the role that commensals could play as a reservoir for their dissemination. © 2008 Journal of Microbiology, Immunology and Infection.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Microbiology, Immunology And Infection, 2008, v. 41 n. 5, p. 428-432 [How to Cite?]
dc.identifier.epage432
dc.identifier.hkuros165738
dc.identifier.isiWOS:000265237900011
dc.identifier.issn1684-1182
2011 Impact Factor: 0.987
2011 SCImago Journal Rankings: 0.101
dc.identifier.issue5
dc.identifier.openurl
dc.identifier.pmid19122926
dc.identifier.scopuseid_2-s2.0-60249100864
dc.identifier.spage428
dc.identifier.urihttp://hdl.handle.net/10722/59397
dc.identifier.volume41
dc.languageeng
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/722895/description#description
dc.publisher.placeHong Kong
dc.relation.ispartofJournal of Microbiology, Immunology and Infection
dc.relation.referencesReferences in Scopus
dc.subject.meshCephalosporin Resistance - genetics
dc.subject.meshCephalosporinase - genetics
dc.subject.meshElectrophoresis, Gel, Pulsed-Field
dc.subject.meshEscherichia coli - enzymology - genetics - isolation & purification
dc.subject.meshFeces - microbiology
dc.subject.meshHumans
dc.subject.meshKlebsiella pneumoniae - enzymology - genetics - isolation & purification
dc.subject.meshMicrobial Sensitivity Tests
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshbeta-Lactam Resistance - genetics
dc.subject.meshbeta-Lactamases - genetics
dc.titleCTX-M type beta-lactamases among fecal Escherichia coli and Klebsiella pneumoniae isolates in non-hospitalized children and adults
dc.typeArticle
Author Affiliations
  1. Tuen Mun Hospital
  2. The University of Hong Kong