File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Value of anaerobic culture in bacterial surveillance program for platelet concentrates

TitleValue of anaerobic culture in bacterial surveillance program for platelet concentrates
Authors
Issue Date2008
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRF
Citation
Transfusion, 2008, v. 48 n. 12, p. 2606-2611 How to Cite?
AbstractBACKGROUND: Short-term aerobic bacterial culture (STABC) has been used routinely in Hong Kong since 1998 to reduce bacterial contamination in platelet concentrates (PCs) with good results. With more countries implementing routine aerobic and anaerobic cultures of PCs, a prospective study was conducted to determine the value of anaerobic culture to STABC. STUDY DESIGN AND METHODS: PC tested by STABC was used as control. Twenty milliliters of the PC selected for this study was aliquoted and pooled for 7 days aerobic and anaerobic culture. If the initial culture was positive, samples retrieved from the original PC and their associated components were cultured for confirmation and microbiologic identification. RESULTS: A total of 10,035 PC units (2007 pools) were tested. The confirmed positive rates by aerobic and anaerobic cultures per pool were 3 (0.15%) and 13 (0.65%), respectively, which was equivalent to an increased yield from 0.03 to 0.13 percent of PC if anaerobic culture was added. Of the 10 bacteria detected by anaerobic culture only, 9 were found to be Propionibacterium acnes and the remaining one Peptostreptococcus sp. Their mean detection time from inoculation was 92.16 hours (range, 50.4-124.8 hr). CONCLUSION: Addition of anaerobic culture to our routine STABC would significantly increase the detection rate of bacterial contaminated PC. However, since only slow-growing bacteria were detected, and because their clinical significance was uncertain, it is concluded that there was no clear justification to introduce anaerobic culture locally if 5-day shelf life for PCs was to be maintained. © 2008 American Association of Blood Banks.
Persistent Identifierhttp://hdl.handle.net/10722/59394
ISSN
2014 Impact Factor: 3.225
2014 SCImago Journal Rankings: 1.144
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, CKen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorLee, KYen_HK
dc.contributor.authorTsui, GTFen_HK
dc.contributor.authorChua, Een_HK
dc.contributor.authorTsoi, WCen_HK
dc.contributor.authorLin, CKen_HK
dc.date.accessioned2010-05-31T03:49:12Z-
dc.date.available2010-05-31T03:49:12Z-
dc.date.issued2008en_HK
dc.identifier.citationTransfusion, 2008, v. 48 n. 12, p. 2606-2611en_HK
dc.identifier.issn0041-1132en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59394-
dc.description.abstractBACKGROUND: Short-term aerobic bacterial culture (STABC) has been used routinely in Hong Kong since 1998 to reduce bacterial contamination in platelet concentrates (PCs) with good results. With more countries implementing routine aerobic and anaerobic cultures of PCs, a prospective study was conducted to determine the value of anaerobic culture to STABC. STUDY DESIGN AND METHODS: PC tested by STABC was used as control. Twenty milliliters of the PC selected for this study was aliquoted and pooled for 7 days aerobic and anaerobic culture. If the initial culture was positive, samples retrieved from the original PC and their associated components were cultured for confirmation and microbiologic identification. RESULTS: A total of 10,035 PC units (2007 pools) were tested. The confirmed positive rates by aerobic and anaerobic cultures per pool were 3 (0.15%) and 13 (0.65%), respectively, which was equivalent to an increased yield from 0.03 to 0.13 percent of PC if anaerobic culture was added. Of the 10 bacteria detected by anaerobic culture only, 9 were found to be Propionibacterium acnes and the remaining one Peptostreptococcus sp. Their mean detection time from inoculation was 92.16 hours (range, 50.4-124.8 hr). CONCLUSION: Addition of anaerobic culture to our routine STABC would significantly increase the detection rate of bacterial contaminated PC. However, since only slow-growing bacteria were detected, and because their clinical significance was uncertain, it is concluded that there was no clear justification to introduce anaerobic culture locally if 5-day shelf life for PCs was to be maintained. © 2008 American Association of Blood Banks.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRFen_HK
dc.relation.ispartofTransfusionen_HK
dc.subject.meshAnaerobiosisen_HK
dc.subject.meshBacteria - growth & development - isolation & purificationen_HK
dc.subject.meshBlood Platelets - microbiologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicrobial Viabilityen_HK
dc.subject.meshPlateletpheresisen_HK
dc.titleValue of anaerobic culture in bacterial surveillance program for platelet concentratesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-1132&volume=48&spage=2606&epage=11&date=2008&atitle=Value+of+anaerobic+culture+in+bacterial+surveillance+program+for+platelet+concentrates.en_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1537-2995.2008.01887.xen_HK
dc.identifier.pmid18694462-
dc.identifier.scopuseid_2-s2.0-57249111793en_HK
dc.identifier.hkuros165739en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57249111793&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume48en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2606en_HK
dc.identifier.epage2611en_HK
dc.identifier.isiWOS:000261439900018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, CK=36087620900en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridLee, KY=7501500790en_HK
dc.identifier.scopusauthoridTsui, GTF=25824458600en_HK
dc.identifier.scopusauthoridChua, E=16199851000en_HK
dc.identifier.scopusauthoridTsoi, WC=36852604400en_HK
dc.identifier.scopusauthoridLin, CK=12752556900en_HK
dc.identifier.citeulike3765365-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats