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Article: Value of anaerobic culture in bacterial surveillance program for platelet concentrates
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TitleValue of anaerobic culture in bacterial surveillance program for platelet concentrates
 
AuthorsLee, CK2 1
Ho, PL1
Lee, KY1
Tsui, GTF1
Chua, E1
Tsoi, WC1
Lin, CK1
 
Issue Date2008
 
PublisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRF
 
CitationTransfusion, 2008, v. 48 n. 12, p. 2606-2611 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1537-2995.2008.01887.x
 
AbstractBACKGROUND: Short-term aerobic bacterial culture (STABC) has been used routinely in Hong Kong since 1998 to reduce bacterial contamination in platelet concentrates (PCs) with good results. With more countries implementing routine aerobic and anaerobic cultures of PCs, a prospective study was conducted to determine the value of anaerobic culture to STABC. STUDY DESIGN AND METHODS: PC tested by STABC was used as control. Twenty milliliters of the PC selected for this study was aliquoted and pooled for 7 days aerobic and anaerobic culture. If the initial culture was positive, samples retrieved from the original PC and their associated components were cultured for confirmation and microbiologic identification. RESULTS: A total of 10,035 PC units (2007 pools) were tested. The confirmed positive rates by aerobic and anaerobic cultures per pool were 3 (0.15%) and 13 (0.65%), respectively, which was equivalent to an increased yield from 0.03 to 0.13 percent of PC if anaerobic culture was added. Of the 10 bacteria detected by anaerobic culture only, 9 were found to be Propionibacterium acnes and the remaining one Peptostreptococcus sp. Their mean detection time from inoculation was 92.16 hours (range, 50.4-124.8 hr). CONCLUSION: Addition of anaerobic culture to our routine STABC would significantly increase the detection rate of bacterial contaminated PC. However, since only slow-growing bacteria were detected, and because their clinical significance was uncertain, it is concluded that there was no clear justification to introduce anaerobic culture locally if 5-day shelf life for PCs was to be maintained. © 2008 American Association of Blood Banks.
 
ISSN0041-1132
2013 Impact Factor: 3.568
 
DOIhttp://dx.doi.org/10.1111/j.1537-2995.2008.01887.x
 
ISI Accession Number IDWOS:000261439900018
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLee, CK
 
dc.contributor.authorHo, PL
 
dc.contributor.authorLee, KY
 
dc.contributor.authorTsui, GTF
 
dc.contributor.authorChua, E
 
dc.contributor.authorTsoi, WC
 
dc.contributor.authorLin, CK
 
dc.date.accessioned2010-05-31T03:49:12Z
 
dc.date.available2010-05-31T03:49:12Z
 
dc.date.issued2008
 
dc.description.abstractBACKGROUND: Short-term aerobic bacterial culture (STABC) has been used routinely in Hong Kong since 1998 to reduce bacterial contamination in platelet concentrates (PCs) with good results. With more countries implementing routine aerobic and anaerobic cultures of PCs, a prospective study was conducted to determine the value of anaerobic culture to STABC. STUDY DESIGN AND METHODS: PC tested by STABC was used as control. Twenty milliliters of the PC selected for this study was aliquoted and pooled for 7 days aerobic and anaerobic culture. If the initial culture was positive, samples retrieved from the original PC and their associated components were cultured for confirmation and microbiologic identification. RESULTS: A total of 10,035 PC units (2007 pools) were tested. The confirmed positive rates by aerobic and anaerobic cultures per pool were 3 (0.15%) and 13 (0.65%), respectively, which was equivalent to an increased yield from 0.03 to 0.13 percent of PC if anaerobic culture was added. Of the 10 bacteria detected by anaerobic culture only, 9 were found to be Propionibacterium acnes and the remaining one Peptostreptococcus sp. Their mean detection time from inoculation was 92.16 hours (range, 50.4-124.8 hr). CONCLUSION: Addition of anaerobic culture to our routine STABC would significantly increase the detection rate of bacterial contaminated PC. However, since only slow-growing bacteria were detected, and because their clinical significance was uncertain, it is concluded that there was no clear justification to introduce anaerobic culture locally if 5-day shelf life for PCs was to be maintained. © 2008 American Association of Blood Banks.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationTransfusion, 2008, v. 48 n. 12, p. 2606-2611 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1537-2995.2008.01887.x
 
dc.identifier.citeulike3765365
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1537-2995.2008.01887.x
 
dc.identifier.epage2611
 
dc.identifier.hkuros165739
 
dc.identifier.isiWOS:000261439900018
 
dc.identifier.issn0041-1132
2013 Impact Factor: 3.568
 
dc.identifier.issue12
 
dc.identifier.openurl
 
dc.identifier.pmid18694462
 
dc.identifier.scopuseid_2-s2.0-57249111793
 
dc.identifier.spage2606
 
dc.identifier.urihttp://hdl.handle.net/10722/59394
 
dc.identifier.volume48
 
dc.languageeng
 
dc.publisherBlackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/TRF
 
dc.publisher.placeUnited States
 
dc.relation.ispartofTransfusion
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnaerobiosis
 
dc.subject.meshBacteria - growth & development - isolation & purification
 
dc.subject.meshBlood Platelets - microbiology
 
dc.subject.meshCells, Cultured
 
dc.subject.meshHumans
 
dc.subject.meshMicrobial Viability
 
dc.subject.meshPlateletpheresis
 
dc.titleValue of anaerobic culture in bacterial surveillance program for platelet concentrates
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Hong Kong Red Cross Blood Transfusion Service