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Article: More and more coronaviruses: Human coronavirus HKU1

TitleMore and more coronaviruses: Human coronavirus HKU1
Authors
KeywordsCoronavirus
Human
HKU1
Novel
Issue Date2009
PublisherMolecular Diversity Preservation International (MDPI) AG.. The Journal's web site is located at http://www.mdpi.com/journal/viruses
Citation
Viruses, 2009, v. 1 n. 1, p. 57-71 How to Cite?
AbstractAfter human coronaviruses OC43, 229E and NL63, human coronavirus HKU1 (HCoV-HKU1) is the fourth human coronavirus discovered. HCoV-HKU1 is a group 2a coronavirus that is still not cultivable. The G + C contents of HCoV-HKU1 genomes are 32%, the lowest among all known coronaviruses with complete genome sequences available. Among all coronaviruses, HCoV-HKU1 shows the most extreme codon usage bias, attributed most importantly to severe cytosine deamination. All HCoV-HKU1 genomes contain unique tandem copies of a 30-base acidic tandem repeat of unknown function at the N-terminus of nsp3 inside the acidic domain upstream of papain-like protease 1. Three genotypes, A, B and C, of HCoV-HKU1 and homologous recombination among their genomes, are observed. The incidence of HCoV-HKU1 infections is the highest in winter. Similar to other human coronaviruses, HCoV-HKU1 infections have been reported globally, with a median (range) incidence of 0.9 (0 - 4.4) %. HCoV-HKU1 is associated with both upper and lower respiratory tract infections that are mostly self-limiting. The most common method for diagnosing HCoV-HKU1 infection is RT-PCR or real-time RT-PCR using RNA extracted from respiratory tract samples such as nasopharyngeal aspirates (NPA). Both the pol and nucleocapsid genes have been used as the targets for amplification. Monoclonal antibodies have been generated for direct antigen detection in NPA. For antibody detection, Escherichia coli BL21 and baculovirus-expressed recombinant nucleocapsid of HCoV-HKU1 have been used for IgG and IgM detection in sera of patients and normal individuals, using Western blot and enzyme-linked immunoassay.
Persistent Identifierhttp://hdl.handle.net/10722/59391
ISSN
2015 Impact Factor: 3.042
2015 SCImago Journal Rankings: 1.873
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYip, CYen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-05-31T03:49:08Z-
dc.date.available2010-05-31T03:49:08Z-
dc.date.issued2009en_HK
dc.identifier.citationViruses, 2009, v. 1 n. 1, p. 57-71en_HK
dc.identifier.issn1999-4915-
dc.identifier.urihttp://hdl.handle.net/10722/59391-
dc.description.abstractAfter human coronaviruses OC43, 229E and NL63, human coronavirus HKU1 (HCoV-HKU1) is the fourth human coronavirus discovered. HCoV-HKU1 is a group 2a coronavirus that is still not cultivable. The G + C contents of HCoV-HKU1 genomes are 32%, the lowest among all known coronaviruses with complete genome sequences available. Among all coronaviruses, HCoV-HKU1 shows the most extreme codon usage bias, attributed most importantly to severe cytosine deamination. All HCoV-HKU1 genomes contain unique tandem copies of a 30-base acidic tandem repeat of unknown function at the N-terminus of nsp3 inside the acidic domain upstream of papain-like protease 1. Three genotypes, A, B and C, of HCoV-HKU1 and homologous recombination among their genomes, are observed. The incidence of HCoV-HKU1 infections is the highest in winter. Similar to other human coronaviruses, HCoV-HKU1 infections have been reported globally, with a median (range) incidence of 0.9 (0 - 4.4) %. HCoV-HKU1 is associated with both upper and lower respiratory tract infections that are mostly self-limiting. The most common method for diagnosing HCoV-HKU1 infection is RT-PCR or real-time RT-PCR using RNA extracted from respiratory tract samples such as nasopharyngeal aspirates (NPA). Both the pol and nucleocapsid genes have been used as the targets for amplification. Monoclonal antibodies have been generated for direct antigen detection in NPA. For antibody detection, Escherichia coli BL21 and baculovirus-expressed recombinant nucleocapsid of HCoV-HKU1 have been used for IgG and IgM detection in sera of patients and normal individuals, using Western blot and enzyme-linked immunoassay.-
dc.languageengen_HK
dc.publisherMolecular Diversity Preservation International (MDPI) AG.. The Journal's web site is located at http://www.mdpi.com/journal/viruses-
dc.relation.ispartofVirusesen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCoronavirus-
dc.subjectHuman-
dc.subjectHKU1-
dc.subjectNovel-
dc.titleMore and more coronaviruses: Human coronavirus HKU1en_HK
dc.typeArticleen_HK
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailYip, CY: h0147879@graduate.hku.hken_HK
dc.identifier.emailHuang, Y: huangyi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYip, CY=rp01721en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/v1010057-
dc.identifier.pmid21994538-
dc.identifier.pmcidPMC3185465-
dc.identifier.hkuros166903en_HK
dc.identifier.volume1-
dc.identifier.issue1-
dc.identifier.spage57-
dc.identifier.epage71-
dc.identifier.isiWOS:000280336800006-
dc.publisher.placeSwitzerland-

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