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Article: Functional significance of skeletal muscle adiponectin production, changes in animal models of obesity and diabetes, and regulation by rosiglitazone treatment

TitleFunctional significance of skeletal muscle adiponectin production, changes in animal models of obesity and diabetes, and regulation by rosiglitazone treatment
Authors
KeywordsAdiponectin
Glucose uptake
Insulin sensitivity
Myokine
Obesity
Issue Date2009
PublisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/
Citation
American Journal Of Physiology - Endocrinology And Metabolism, 2009, v. 297 n. 3, p. E657-E664 How to Cite?
AbstractEndocrine effects of adipose-derived adiponectin on skeletal muscle have been shown to account, at least in part, for the anti-diabetic effects of this adipokine. Recently, the concept of myokines has gained credence, and the potential for skeletal muscle to produce adiponectin has been suggested. Here we demonstrated an increased level of adiponectin mRNA and protein expression as well as protein secretion in response to rosiglitazone treatment in L6 muscle cells. This correlated with the ability of rosiglitazone to enhance insulin sensitivity for stimulation of protein kinase B (Akt) phosphorylation and glucose transport; rosiglitazone also corrected high-glucose-induced insulin resistance in L6 cells. Overexpression of adiponectin confirmed the functional significance of local production of adiponectin in muscle cells via elevated glucose uptake and increased insulin sensitivity. In obese diabetic db/db mice, there was a change in the adiponectin expression profile in soleus and extensor digitorum longus (EDL) muscle with less high molecular weight (HMW) and more medium (MMW)/low (LMW) molecular weight species detected. Induction of obesity and insulin resistance in rats by feeding a high-fat high-sucrose diet also led to decreased muscle HMW adiponectin content that could be corrected by rosiglitazone treatment. In summary, we show the ability of skeletal muscle cells to produce adiponectin, which can mediate autocrine metabolic effects, thus establishing adiponectin as a bona fide myokine. We also demonstrate that skeletal muscle adiponectin production is altered in animal models of obesity and diabetes and that these changes can be corrected by rosiglitazone. Copyright © 2009 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/59342
ISSN
2015 Impact Factor: 3.825
2015 SCImago Journal Rankings: 2.465
ISI Accession Number ID
Funding AgencyGrant Number
Canadian Institutes of Health Research (CIHR)
Fonds de la Recherche en Sante du Quebec National Scientist Award
Funding Information:

This work was supported by an operating grant to G. Sweeney from the Canadian Institutes of Health Research (CIHR) as well as a CIHR New Investigator Award to GS. AM holds an Fonds de la Recherche en Sante du Quebec National Scientist Award. Part of this work was also supported by a CIHR-Tekes team grant (A. Marette and G. Sweeney).

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorChewchuk, Sen_HK
dc.contributor.authorLavigne, Cen_HK
dc.contributor.authorBrûlé, Sen_HK
dc.contributor.authorPilon, Gen_HK
dc.contributor.authorHoude, Ven_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorMarette, Aen_HK
dc.contributor.authorSweeney, Gen_HK
dc.date.accessioned2010-05-31T03:48:04Z-
dc.date.available2010-05-31T03:48:04Z-
dc.date.issued2009en_HK
dc.identifier.citationAmerican Journal Of Physiology - Endocrinology And Metabolism, 2009, v. 297 n. 3, p. E657-E664en_HK
dc.identifier.issn0193-1849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59342-
dc.description.abstractEndocrine effects of adipose-derived adiponectin on skeletal muscle have been shown to account, at least in part, for the anti-diabetic effects of this adipokine. Recently, the concept of myokines has gained credence, and the potential for skeletal muscle to produce adiponectin has been suggested. Here we demonstrated an increased level of adiponectin mRNA and protein expression as well as protein secretion in response to rosiglitazone treatment in L6 muscle cells. This correlated with the ability of rosiglitazone to enhance insulin sensitivity for stimulation of protein kinase B (Akt) phosphorylation and glucose transport; rosiglitazone also corrected high-glucose-induced insulin resistance in L6 cells. Overexpression of adiponectin confirmed the functional significance of local production of adiponectin in muscle cells via elevated glucose uptake and increased insulin sensitivity. In obese diabetic db/db mice, there was a change in the adiponectin expression profile in soleus and extensor digitorum longus (EDL) muscle with less high molecular weight (HMW) and more medium (MMW)/low (LMW) molecular weight species detected. Induction of obesity and insulin resistance in rats by feeding a high-fat high-sucrose diet also led to decreased muscle HMW adiponectin content that could be corrected by rosiglitazone treatment. In summary, we show the ability of skeletal muscle cells to produce adiponectin, which can mediate autocrine metabolic effects, thus establishing adiponectin as a bona fide myokine. We also demonstrate that skeletal muscle adiponectin production is altered in animal models of obesity and diabetes and that these changes can be corrected by rosiglitazone. Copyright © 2009 the American Physiological Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://ajpendo.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Endocrinology and Metabolismen_HK
dc.rightsAmerican Journal of Physiology: Endocrinology and Metabolism. Copyright © American Physiological Society.-
dc.rightsThis is an unofficial adaptation or translation of an article that appeared in a publication of the American Physiological Society. The American Physiological Society has not endorsed the content of this adaptation or translation, or the context of its use.-
dc.subjectAdiponectinen_HK
dc.subjectGlucose uptakeen_HK
dc.subjectInsulin sensitivityen_HK
dc.subjectMyokineen_HK
dc.subjectObesityen_HK
dc.subject.meshAdiponectin - genetics - metabolism - physiology-
dc.subject.meshDiabetes Mellitus, Experimental - blood - drug therapy - genetics - metabolism-
dc.subject.meshMuscle, Skeletal - drug effects - metabolism - physiology-
dc.subject.meshObesity - blood - drug therapy - genetics - metabolism-
dc.subject.meshThiazolidinediones - pharmacology - therapeutic use-
dc.titleFunctional significance of skeletal muscle adiponectin production, changes in animal models of obesity and diabetes, and regulation by rosiglitazone treatmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0193-1849&volume=297&issue=3&spage=E657&epage=E664&date=2009&atitle=Functional+significance+of+skeletal+muscle+adiponectin+production,+changes+in+animal+models+of+obesity+and+diabetes,+and+regulation+by+rosiglitazone+treatmenten_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1152/ajpendo.00186.2009en_HK
dc.identifier.pmid19531641-
dc.identifier.scopuseid_2-s2.0-69049113073en_HK
dc.identifier.hkuros158006en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69049113073&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume297en_HK
dc.identifier.issue3en_HK
dc.identifier.spageE657en_HK
dc.identifier.epageE664en_HK
dc.identifier.isiWOS:000269063000011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, Y=26643544200en_HK
dc.identifier.scopusauthoridChewchuk, S=33367555600en_HK
dc.identifier.scopusauthoridLavigne, C=7004416894en_HK
dc.identifier.scopusauthoridBrûlé, S=6506639591en_HK
dc.identifier.scopusauthoridPilon, G=6506554128en_HK
dc.identifier.scopusauthoridHoude, V=14522659300en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridMarette, A=7005877156en_HK
dc.identifier.scopusauthoridSweeney, G=7102852659en_HK

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