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Article: Glycoxidized LDL increases lectin-like oxidized low density lipoprotein receptor-1 in diabetes mellitus

TitleGlycoxidized LDL increases lectin-like oxidized low density lipoprotein receptor-1 in diabetes mellitus
Authors
Issue Date2009
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosis
Citation
Atherosclerosis, 2009, v. 203 n. 2, p. 522-527 How to Cite?
AbstractAims: LDL is subjected to glycoxidation in diabetes mellitus. We have evaluated the effect of glycoxidized LDL on lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression in endothelial cells in vitro, as well as the relationship between glycoxidzied LDL and LOX-1 in type 2 diabetic patients with and without microalbuminuria in vivo. Methods: Endothelial cells were incubated with modified LDL including glycoxidized LDL, oxidized LDL (oxLDL), glycated LDL, and acetylated LDL, and cellular LOX-1 and the soluble forms of LOX-1 (sLOX-1) in cell medium was measured. Glycoxidized LDL in diabetic patients was determined by measuring the glycoxidation product Nε-(carboxymethyl)lysine (CML) in apolipoprotein (apo) B. Serum oxLDL and sLOX-1 was determined by ELISA. Results: Only glycoxidized LDL and oxLDL significantly increased LOX-1 expression (p < 0.05) and the production of sLOX-1 (p < 0.05), and the effect of glycoxidized LDL was greater than that of oxLDL. Both normoalbuminuric (n = 110) and microalbuminuric (n = 91) patients had higher serum apoB-CML than controls (n = 105) (p < 0.01), but oxLDL was only elevated in the microalbuminuric patients (p < 0.05). Serum sLOX-1 was significantly increased in both groups of patients compared to controls (p < 0.01). Serum sLOX-1 correlated with apoB-CML (r = 0.36, p < 0.001) but not with oxLDL. The relationship between sLOX-1 and apoB-CML was independent of HbA1c, age, gender, BMI and smoking status. Conclusion: Glycoxidized LDL was more potent than oxLDL in inducing LOX-1 in vitro. Serum concentration of apoB-CML, a marker of glycoxidized LDL, was increased in type 2 diabetic patients with and without microalbuminuria, and this was associated with an increase in serum sLOX-1. © 2008 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59295
ISSN
2015 Impact Factor: 3.942
2015 SCImago Journal Rankings: 1.819
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7585/05M
Funding Information:

This study is supported by funding from the Hong Kong Research Grants Council (HKU 7585/05M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorShiu, SWMen_HK
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorWong, Yen_HK
dc.contributor.authorLeng, Len_HK
dc.contributor.authorBucala, Ren_HK
dc.date.accessioned2010-05-31T03:47:11Z-
dc.date.available2010-05-31T03:47:11Z-
dc.date.issued2009en_HK
dc.identifier.citationAtherosclerosis, 2009, v. 203 n. 2, p. 522-527en_HK
dc.identifier.issn0021-9150en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59295-
dc.description.abstractAims: LDL is subjected to glycoxidation in diabetes mellitus. We have evaluated the effect of glycoxidized LDL on lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) expression in endothelial cells in vitro, as well as the relationship between glycoxidzied LDL and LOX-1 in type 2 diabetic patients with and without microalbuminuria in vivo. Methods: Endothelial cells were incubated with modified LDL including glycoxidized LDL, oxidized LDL (oxLDL), glycated LDL, and acetylated LDL, and cellular LOX-1 and the soluble forms of LOX-1 (sLOX-1) in cell medium was measured. Glycoxidized LDL in diabetic patients was determined by measuring the glycoxidation product Nε-(carboxymethyl)lysine (CML) in apolipoprotein (apo) B. Serum oxLDL and sLOX-1 was determined by ELISA. Results: Only glycoxidized LDL and oxLDL significantly increased LOX-1 expression (p < 0.05) and the production of sLOX-1 (p < 0.05), and the effect of glycoxidized LDL was greater than that of oxLDL. Both normoalbuminuric (n = 110) and microalbuminuric (n = 91) patients had higher serum apoB-CML than controls (n = 105) (p < 0.01), but oxLDL was only elevated in the microalbuminuric patients (p < 0.05). Serum sLOX-1 was significantly increased in both groups of patients compared to controls (p < 0.01). Serum sLOX-1 correlated with apoB-CML (r = 0.36, p < 0.001) but not with oxLDL. The relationship between sLOX-1 and apoB-CML was independent of HbA1c, age, gender, BMI and smoking status. Conclusion: Glycoxidized LDL was more potent than oxLDL in inducing LOX-1 in vitro. Serum concentration of apoB-CML, a marker of glycoxidized LDL, was increased in type 2 diabetic patients with and without microalbuminuria, and this was associated with an increase in serum sLOX-1. © 2008 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/atherosclerosisen_HK
dc.relation.ispartofAtherosclerosisen_HK
dc.rightsAtherosclerosis. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAlbuminuria - blooden_HK
dc.subject.meshApolipoproteins B - metabolismen_HK
dc.subject.meshDiabetes Mellitus, Type 2 - blooden_HK
dc.subject.meshEndothelial Cells - metabolismen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGlycosylationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLipoproteins, LDL - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshModels, Biologicalen_HK
dc.subject.meshScavenger Receptors, Class E - metabolismen_HK
dc.titleGlycoxidized LDL increases lectin-like oxidized low density lipoprotein receptor-1 in diabetes mellitusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9150&volume=203&issue=2&spage=522&epage=7&date=2009&atitle=Glycoxidized+LDL+increases+lectin-like+oxidized+low+density+lipoprotein+receptor-1+in+diabetes+mellitusen_HK
dc.identifier.emailTan, KCB:kcbtan@hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.atherosclerosis.2008.07.012en_HK
dc.identifier.pmid18755461-
dc.identifier.scopuseid_2-s2.0-62649164710en_HK
dc.identifier.hkuros158341en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62649164710&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume203en_HK
dc.identifier.issue2en_HK
dc.identifier.spage522en_HK
dc.identifier.epage527en_HK
dc.identifier.isiWOS:000265464800030-
dc.publisher.placeIrelanden_HK
dc.relation.projectGlycosidized LDL and atherosclerosis-
dc.identifier.scopusauthoridShiu, SWM=7005550652en_HK
dc.identifier.scopusauthoridTan, KCB=8082703100en_HK
dc.identifier.scopusauthoridWong, Y=24073787400en_HK
dc.identifier.scopusauthoridLeng, L=7006089148en_HK
dc.identifier.scopusauthoridBucala, R=7102379822en_HK

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