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Article: The role of survivin2 in primitive hematopoiesis during zebrafish development
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TitleThe role of survivin2 in primitive hematopoiesis during zebrafish development
 
AuthorsMa, ACH1
Chung, MIS1
Liang, R1
Leung, AYH1
 
Issue Date2009
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
 
CitationLeukemia, 2009, v. 23 n. 4, p. 712-720 [How to Cite?]
DOI: http://dx.doi.org/10.1038/leu.2008.363
 
AbstractSurvivin is an inhibitor of apoptosis and its role in embryonic development is not completely understood. In zebrafish, survivin undergoes gene duplication. Survivin1 (sur1) has been shown to mediate angiogenesis but not hematopoiesis. In this study, we examined survivin2 (sur2) with particular reference to its role in primitive hematopoiesis during zebrafish development. sur2 was expressed predominantly in the intermediate cell mass (ICM, site of primitive hematopoiesis). Morpholino (MO) targeting at intron1-exon2 junction of sur2 significantly reduced green fluorescent protein+ (erythroid) cell population in transgenic Tg (gata1:gfp) embryos at 18h post-fertilization (h.p.f.; wild type: 4.49±0.15%; Sur2MO embryos: 2.22±0.12%, P=0.02). Molecular targeting was confirmed by reverse transcription-PCR and MO specificity by successful sur2 mRNA rescue. sur2 MO also downregulated genes associated with hematopoietic stem cells (scl, lmo2), erythroid (gata1, α- and β-embryonic hemoglobins) as well as early (pu.1) and late (mpo, l-plastin) myelomonocytic lineages at 12 and 18h.p.f. This was associated with an increase in apoptosis in the ICM and alteration of cell-cycle status of erythroid cells. Both effects were caspase dependent. In conclusion, sur2 is important in maintaining hematopoietic stem and lineage committed cells during zebrafish development, by virtue of its antiapoptotic activity in a caspase dependent and cell autonomous fashion.
 
ISSN0887-6924
2013 Impact Factor: 9.379
2013 SCImago Journal Rankings: 4.500
 
DOIhttp://dx.doi.org/10.1038/leu.2008.363
 
ISI Accession Number IDWOS:000265220800012
Funding AgencyGrant Number
Competitive Earmarked ResearchHKU 7520/06M
HKU 7488/04M
HKU 7538/05M
HKU
Funding Information:

We thank Jessie Fu and Babs Kwok for performing some of the microinjection experiments and to Mr Howard Chow for part of the molecular studies. We also thank Dr Anming Meng (Tsinghua University, China) for the generous gift of the Tg(gata1:gfp) fish lines. We also thank the live cell imaging core facility of the Department of Anatomy for the confocal microscopy. This work was supported by the Competitive Earmarked Research Grants (CERG; HKU 7520/06M, HKU 7488/04M, HKU 7538/05M) and an internal research grant from HKU.

 
ReferencesReferences in Scopus
 
GrantsA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation
The roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model
 
DC FieldValue
dc.contributor.authorMa, ACH
 
dc.contributor.authorChung, MIS
 
dc.contributor.authorLiang, R
 
dc.contributor.authorLeung, AYH
 
dc.date.accessioned2010-05-31T03:46:52Z
 
dc.date.available2010-05-31T03:46:52Z
 
dc.date.issued2009
 
dc.description.abstractSurvivin is an inhibitor of apoptosis and its role in embryonic development is not completely understood. In zebrafish, survivin undergoes gene duplication. Survivin1 (sur1) has been shown to mediate angiogenesis but not hematopoiesis. In this study, we examined survivin2 (sur2) with particular reference to its role in primitive hematopoiesis during zebrafish development. sur2 was expressed predominantly in the intermediate cell mass (ICM, site of primitive hematopoiesis). Morpholino (MO) targeting at intron1-exon2 junction of sur2 significantly reduced green fluorescent protein+ (erythroid) cell population in transgenic Tg (gata1:gfp) embryos at 18h post-fertilization (h.p.f.; wild type: 4.49±0.15%; Sur2MO embryos: 2.22±0.12%, P=0.02). Molecular targeting was confirmed by reverse transcription-PCR and MO specificity by successful sur2 mRNA rescue. sur2 MO also downregulated genes associated with hematopoietic stem cells (scl, lmo2), erythroid (gata1, α- and β-embryonic hemoglobins) as well as early (pu.1) and late (mpo, l-plastin) myelomonocytic lineages at 12 and 18h.p.f. This was associated with an increase in apoptosis in the ICM and alteration of cell-cycle status of erythroid cells. Both effects were caspase dependent. In conclusion, sur2 is important in maintaining hematopoietic stem and lineage committed cells during zebrafish development, by virtue of its antiapoptotic activity in a caspase dependent and cell autonomous fashion.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationLeukemia, 2009, v. 23 n. 4, p. 712-720 [How to Cite?]
DOI: http://dx.doi.org/10.1038/leu.2008.363
 
dc.identifier.citeulike3864492
 
dc.identifier.doihttp://dx.doi.org/10.1038/leu.2008.363
 
dc.identifier.epage720
 
dc.identifier.hkuros157674
 
dc.identifier.isiWOS:000265220800012
Funding AgencyGrant Number
Competitive Earmarked ResearchHKU 7520/06M
HKU 7488/04M
HKU 7538/05M
HKU
Funding Information:

We thank Jessie Fu and Babs Kwok for performing some of the microinjection experiments and to Mr Howard Chow for part of the molecular studies. We also thank Dr Anming Meng (Tsinghua University, China) for the generous gift of the Tg(gata1:gfp) fish lines. We also thank the live cell imaging core facility of the Department of Anatomy for the confocal microscopy. This work was supported by the Competitive Earmarked Research Grants (CERG; HKU 7520/06M, HKU 7488/04M, HKU 7538/05M) and an internal research grant from HKU.

 
dc.identifier.issn0887-6924
2013 Impact Factor: 9.379
2013 SCImago Journal Rankings: 4.500
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid19151781
 
dc.identifier.scopuseid_2-s2.0-64849086155
 
dc.identifier.spage712
 
dc.identifier.urihttp://hdl.handle.net/10722/59279
 
dc.identifier.volume23
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLeukemia
 
dc.relation.projectA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation
 
dc.relation.projectThe roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshApoptosis
 
dc.subject.meshApoptosis Regulatory Proteins
 
dc.subject.meshCaspases
 
dc.subject.meshCell Lineage
 
dc.subject.meshEmbryo, Nonmammalian
 
dc.subject.meshEmbryonic Development
 
dc.subject.meshHematopoiesis
 
dc.subject.meshHematopoietic Stem Cells - cytology
 
dc.subject.meshMicrotubule-Associated Proteins - physiology
 
dc.subject.meshZebrafish
 
dc.subject.meshZebrafish Proteins - physiology
 
dc.titleThe role of survivin2 in primitive hematopoiesis during zebrafish development
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong