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Article: The role of survivin2 in primitive hematopoiesis during zebrafish development

TitleThe role of survivin2 in primitive hematopoiesis during zebrafish development
Authors
Issue Date2009
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 2009, v. 23 n. 4, p. 712-720 How to Cite?
Abstract
Survivin is an inhibitor of apoptosis and its role in embryonic development is not completely understood. In zebrafish, survivin undergoes gene duplication. Survivin1 (sur1) has been shown to mediate angiogenesis but not hematopoiesis. In this study, we examined survivin2 (sur2) with particular reference to its role in primitive hematopoiesis during zebrafish development. sur2 was expressed predominantly in the intermediate cell mass (ICM, site of primitive hematopoiesis). Morpholino (MO) targeting at intron1-exon2 junction of sur2 significantly reduced green fluorescent protein+ (erythroid) cell population in transgenic Tg (gata1:gfp) embryos at 18h post-fertilization (h.p.f.; wild type: 4.49±0.15%; Sur2MO embryos: 2.22±0.12%, P=0.02). Molecular targeting was confirmed by reverse transcription-PCR and MO specificity by successful sur2 mRNA rescue. sur2 MO also downregulated genes associated with hematopoietic stem cells (scl, lmo2), erythroid (gata1, α- and β-embryonic hemoglobins) as well as early (pu.1) and late (mpo, l-plastin) myelomonocytic lineages at 12 and 18h.p.f. This was associated with an increase in apoptosis in the ICM and alteration of cell-cycle status of erythroid cells. Both effects were caspase dependent. In conclusion, sur2 is important in maintaining hematopoietic stem and lineage committed cells during zebrafish development, by virtue of its antiapoptotic activity in a caspase dependent and cell autonomous fashion.
Persistent Identifierhttp://hdl.handle.net/10722/59279
ISSN
2013 Impact Factor: 9.379
2013 SCImago Journal Rankings: 4.500
ISI Accession Number ID
Funding AgencyGrant Number
Competitive Earmarked ResearchHKU 7520/06M
HKU 7488/04M
HKU 7538/05M
HKU
Funding Information:

We thank Jessie Fu and Babs Kwok for performing some of the microinjection experiments and to Mr Howard Chow for part of the molecular studies. We also thank Dr Anming Meng (Tsinghua University, China) for the generous gift of the Tg(gata1:gfp) fish lines. We also thank the live cell imaging core facility of the Department of Anatomy for the confocal microscopy. This work was supported by the Competitive Earmarked Research Grants (CERG; HKU 7520/06M, HKU 7488/04M, HKU 7538/05M) and an internal research grant from HKU.

References
Grants

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorMa, ACHen_HK
dc.contributor.authorChung, MISen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-05-31T03:46:52Z-
dc.date.available2010-05-31T03:46:52Z-
dc.date.issued2009en_HK
dc.identifier.citationLeukemia, 2009, v. 23 n. 4, p. 712-720en_HK
dc.identifier.issn0887-6924en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59279-
dc.description.abstractSurvivin is an inhibitor of apoptosis and its role in embryonic development is not completely understood. In zebrafish, survivin undergoes gene duplication. Survivin1 (sur1) has been shown to mediate angiogenesis but not hematopoiesis. In this study, we examined survivin2 (sur2) with particular reference to its role in primitive hematopoiesis during zebrafish development. sur2 was expressed predominantly in the intermediate cell mass (ICM, site of primitive hematopoiesis). Morpholino (MO) targeting at intron1-exon2 junction of sur2 significantly reduced green fluorescent protein+ (erythroid) cell population in transgenic Tg (gata1:gfp) embryos at 18h post-fertilization (h.p.f.; wild type: 4.49±0.15%; Sur2MO embryos: 2.22±0.12%, P=0.02). Molecular targeting was confirmed by reverse transcription-PCR and MO specificity by successful sur2 mRNA rescue. sur2 MO also downregulated genes associated with hematopoietic stem cells (scl, lmo2), erythroid (gata1, α- and β-embryonic hemoglobins) as well as early (pu.1) and late (mpo, l-plastin) myelomonocytic lineages at 12 and 18h.p.f. This was associated with an increase in apoptosis in the ICM and alteration of cell-cycle status of erythroid cells. Both effects were caspase dependent. In conclusion, sur2 is important in maintaining hematopoietic stem and lineage committed cells during zebrafish development, by virtue of its antiapoptotic activity in a caspase dependent and cell autonomous fashion.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leuen_HK
dc.relation.ispartofLeukemiaen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshApoptosisen_HK
dc.subject.meshApoptosis Regulatory Proteinsen_HK
dc.subject.meshCaspasesen_HK
dc.subject.meshCell Lineageen_HK
dc.subject.meshEmbryo, Nonmammalianen_HK
dc.subject.meshEmbryonic Developmenten_HK
dc.subject.meshHematopoiesisen_HK
dc.subject.meshHematopoietic Stem Cells - cytologyen_HK
dc.subject.meshMicrotubule-Associated Proteins - physiologyen_HK
dc.subject.meshZebrafishen_HK
dc.subject.meshZebrafish Proteins - physiologyen_HK
dc.titleThe role of survivin2 in primitive hematopoiesis during zebrafish developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0887-6924&volume=23&issue=4&spage=712&epage=720&date=2009&atitle=The+role+of+survivin2+in+primitive+hematopoiesis+during+zebrafish+development.+en_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/leu.2008.363en_HK
dc.identifier.pmid19151781en_HK
dc.identifier.scopuseid_2-s2.0-64849086155en_HK
dc.identifier.hkuros157674en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64849086155&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue4en_HK
dc.identifier.spage712en_HK
dc.identifier.epage720en_HK
dc.identifier.isiWOS:000265220800012-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectA study of normal and deregulated haematopoiesis in zebrafish, with special reference to the role of BMP signaling in haematopoietic stem cell proliferation-
dc.relation.projectThe roles of survivin in hematopoiesis, angiogenesis and tumorigenesis in a zebrafish knock-down and transgenic model-
dc.identifier.scopusauthoridMa, ACH=15849157500en_HK
dc.identifier.scopusauthoridChung, MIS=25958659100en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike3864492-

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