File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Neurogenesis and angiogenesis within the ipsilateral thalamus with secondary damage after focal cortical infarction in hypertensive rats

TitleNeurogenesis and angiogenesis within the ipsilateral thalamus with secondary damage after focal cortical infarction in hypertensive rats
Authors
KeywordsAngiogenesis
Focal ischemia
Neurogenesis
Rat
Issue Date2009
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/jcbfm
Citation
Journal Of Cerebral Blood Flow And Metabolism, 2009, v. 29 n. 9, p. 1538-1546 How to Cite?
AbstractNeurogenesis and angiogenesis in the subventricular zone and peri-infarct region have been confirmed. However, newly formed neuronal cells and blood vessels that appear in the nonischemic ipsilateral ventroposterior nucleus (VPN) of the thalamus with secondary damage after stroke has not been previously studied. Twenty-four stroke-prone renovascular hypertensive rats were subjected to distal right middle cerebral artery occlusion (MCAO) or sham operation. 5′-Bromo-2′-deoxyuridine (BrdU) was used to label cell proliferation. Rats were killed at 7 or 14 days after the operation. Neuronal nuclei (NeuN), OX-42, BrdU, nestin, laminin+, BrdU +/nestin+, BrdU+/NeuN+, nestin +/GFAP+(glial fibrillary acidic protein), and BrdU +/laminin+ immunoreactive cells were detected within the ipsilateral VPN. The primary infarction was confined to the right somatosensory cortex. Within the ipsilateral VPN of the ischemic rats, the number of NeuN + neurons decreased, the OX-42+ microglia cells were activated, and BrdU+ and nestin+ cells were detected at day 7 after MCAO and increased in number at day 14. Moreover, BrdU +/nestin+ cells and BrdU+/NeuN+ cells were detected at day 14 after MCAO. In addition, the ischemic rats showed a significant increase in vascular density in the ipsilateral VPN compared with the sham-operated rats. These results suggest that secondary damage with neurogenesis and angiogenesis of the ipsilateral VPN of the thalamus occurs after focal cortical infarction. © 2009 ISCBFM All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59234
ISSN
2015 Impact Factor: 4.929
2015 SCImago Journal Rankings: 3.004
ISI Accession Number ID
Funding AgencyGrant Number
Higher Education Institutions of the Ministry of Education, China
National Natural Science Foundation of China39940012
30271485
30770764
Natural Science Foundation of Guangdong Province, China990065
21906
2003C30610
2003B30303
2003D30301
China Medical Board of New York Inc.CMB00-730
Fund for Priority Subjects in Clinical Medicine, Chinese Ministry of Health
First Affiliated Hospital and Life Science Institute in Sun Yet-Sen University
Scientific Project of Guangzhou City2006 Z1-E0111
Z1-E0113
Funding Information:

This study was supported by the grants from the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of the Ministry of Education, China (2002), the National Natural Science Foundation of China (Nos. 39940012, 30271485, and 30770764), the Natural Science Foundation of Guangdong Province, China (Nos. 990065, 21906, and 2003C30610), China Medical Board of New York Inc. (No. CMB00-730), the Fund for Priority Subjects in Clinical Medicine, Chinese Ministry of Health (2004), the Key and Scientific Project of the Natural Science Foundation of Guangdong Province, China (Nos. 2003B30303, 2003C30610, and 2003D30301), and the fund on collaboration study for First Affiliated Hospital and Life Science Institute in Sun Yet-Sen University (2006), the Scientific Project of Guangzhou City (Nos. 2006 Z1-E0111 and Z1-E0113).

References

 

DC FieldValueLanguage
dc.contributor.authorLing, Len_HK
dc.contributor.authorZeng, Jen_HK
dc.contributor.authorPei, Zen_HK
dc.contributor.authorCheung, RTFen_HK
dc.contributor.authorHou, Qen_HK
dc.contributor.authorXing, Sen_HK
dc.contributor.authorZhang, Sen_HK
dc.date.accessioned2010-05-31T03:45:47Z-
dc.date.available2010-05-31T03:45:47Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Cerebral Blood Flow And Metabolism, 2009, v. 29 n. 9, p. 1538-1546en_HK
dc.identifier.issn0271-678Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/59234-
dc.description.abstractNeurogenesis and angiogenesis in the subventricular zone and peri-infarct region have been confirmed. However, newly formed neuronal cells and blood vessels that appear in the nonischemic ipsilateral ventroposterior nucleus (VPN) of the thalamus with secondary damage after stroke has not been previously studied. Twenty-four stroke-prone renovascular hypertensive rats were subjected to distal right middle cerebral artery occlusion (MCAO) or sham operation. 5′-Bromo-2′-deoxyuridine (BrdU) was used to label cell proliferation. Rats were killed at 7 or 14 days after the operation. Neuronal nuclei (NeuN), OX-42, BrdU, nestin, laminin+, BrdU +/nestin+, BrdU+/NeuN+, nestin +/GFAP+(glial fibrillary acidic protein), and BrdU +/laminin+ immunoreactive cells were detected within the ipsilateral VPN. The primary infarction was confined to the right somatosensory cortex. Within the ipsilateral VPN of the ischemic rats, the number of NeuN + neurons decreased, the OX-42+ microglia cells were activated, and BrdU+ and nestin+ cells were detected at day 7 after MCAO and increased in number at day 14. Moreover, BrdU +/nestin+ cells and BrdU+/NeuN+ cells were detected at day 14 after MCAO. In addition, the ischemic rats showed a significant increase in vascular density in the ipsilateral VPN compared with the sham-operated rats. These results suggest that secondary damage with neurogenesis and angiogenesis of the ipsilateral VPN of the thalamus occurs after focal cortical infarction. © 2009 ISCBFM All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/jcbfmen_HK
dc.relation.ispartofJournal of Cerebral Blood Flow and Metabolismen_HK
dc.subjectAngiogenesisen_HK
dc.subjectFocal ischemiaen_HK
dc.subjectNeurogenesisen_HK
dc.subjectRaten_HK
dc.subject.meshCerebral Cortex - anatomy and histology - pathology-
dc.subject.meshCerebral Infarction - pathology - physiopathology-
dc.subject.meshHypertension - pathology - physiopathology-
dc.subject.meshNeovascularization, Physiologic - physiology-
dc.subject.meshThalamus - anatomy and histology - pathology - physiology-
dc.titleNeurogenesis and angiogenesis within the ipsilateral thalamus with secondary damage after focal cortical infarction in hypertensive ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0271-678X&volume=40&spage=&epage=&date=2009&atitle=Neurogenesis+and+angiogenesis+within+the+ipsilateral+thalamus+with+secondary+damage+following+focal+cortical+infarction+in+hypertensive+ratsen_HK
dc.identifier.emailCheung, RTF:rtcheung@hku.hken_HK
dc.identifier.authorityCheung, RTF=rp00434en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/jcbfm.2009.76en_HK
dc.identifier.pmid19536072-
dc.identifier.scopuseid_2-s2.0-69549135254en_HK
dc.identifier.hkuros160471en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69549135254&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1538en_HK
dc.identifier.epage1546en_HK
dc.identifier.isiWOS:000269447600005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLing, L=23395141400en_HK
dc.identifier.scopusauthoridZeng, J=7402652310en_HK
dc.identifier.scopusauthoridPei, Z=23051646800en_HK
dc.identifier.scopusauthoridCheung, RTF=7202397498en_HK
dc.identifier.scopusauthoridHou, Q=23050651700en_HK
dc.identifier.scopusauthoridXing, S=23979049900en_HK
dc.identifier.scopusauthoridZhang, S=12766002100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats