File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of ion channels in human preadipocytes

TitleCharacterization of ion channels in human preadipocytes
Authors
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 2009, v. 218 n. 2, p. 427-435 How to Cite?
Abstract
Ion channels participate in regulation of cell proliferation. However, though preadipocyte (the progenitor of fat cell) is a type of highly proliferating cells, ion channel expression and their role in proliferation is not understood in human preadipocytes. The present study was designed to characterize ion channels using whole-cell patch clamp technique, RT-PCR, and Western blotting. It was found that a 4-aminopyridine- (4-AP) sensitive transient outward K + current (I to) was present in a small population of (32.0%) cells, and an outward "noisy" big conductance Ca 2+-activated K + current (I KCa) was present in most (92.7%) preadipocytes. The noisy current was inhibited by the big conductance I KCa channel blocker paxilline (1 μM), and enhanced by the Ca 2+ ionophore A23187 (5 μM) and the big conductance I KCa channel activator NS1619 (10 μM). RT-PCR and Western blot revealed the molecular identities (i.e., KCa1.1 and Kv4.2) of the functional ionic currents I KCa and I to. Blockade of I KCa or I to with paxilline or 4-AP reduced preadipocyte proliferation, and similar results were obtained with specific siRNAs targeting to KCa1.1 and Kv4.2. Flow cytometric analysis showed ion channel blockade or knockdown of KCa1.1 or Kv4.2 with specific siRNA increased the cell number of G0/G1 phase. The present study demonstrates for the first time that two types of functional ion channel currents, I to and big conductance I KCa, are present in human preadipocytes and that these two types of ion channels participate in regulating proliferation of human preadipocytes. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/59219
ISSN
2013 Impact Factor: 3.874
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

The study was supported by a seeding grant from Committee on Research and Conference Grants of University of Hong Kong. The authors thank Mr. Kevin Lai for his excellent technical assistance.

References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Xi'an Jiaotong University, School of Medicine
  3. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorHu, Hen_HK
dc.contributor.authorHe, MLen_HK
dc.contributor.authorTao, Ren_HK
dc.contributor.authorSun, HYen_HK
dc.contributor.authorHu, Ren_HK
dc.contributor.authorZang, WJen_HK
dc.contributor.authorYuan, BXen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-05-31T03:45:23Z-
dc.date.available2010-05-31T03:45:23Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Cellular Physiology, 2009, v. 218 n. 2, p. 427-435en_HK
dc.identifier.issn0021-9541en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59219-
dc.description.abstractIon channels participate in regulation of cell proliferation. However, though preadipocyte (the progenitor of fat cell) is a type of highly proliferating cells, ion channel expression and their role in proliferation is not understood in human preadipocytes. The present study was designed to characterize ion channels using whole-cell patch clamp technique, RT-PCR, and Western blotting. It was found that a 4-aminopyridine- (4-AP) sensitive transient outward K + current (I to) was present in a small population of (32.0%) cells, and an outward "noisy" big conductance Ca 2+-activated K + current (I KCa) was present in most (92.7%) preadipocytes. The noisy current was inhibited by the big conductance I KCa channel blocker paxilline (1 μM), and enhanced by the Ca 2+ ionophore A23187 (5 μM) and the big conductance I KCa channel activator NS1619 (10 μM). RT-PCR and Western blot revealed the molecular identities (i.e., KCa1.1 and Kv4.2) of the functional ionic currents I KCa and I to. Blockade of I KCa or I to with paxilline or 4-AP reduced preadipocyte proliferation, and similar results were obtained with specific siRNAs targeting to KCa1.1 and Kv4.2. Flow cytometric analysis showed ion channel blockade or knockdown of KCa1.1 or Kv4.2 with specific siRNA increased the cell number of G0/G1 phase. The present study demonstrates for the first time that two types of functional ion channel currents, I to and big conductance I KCa, are present in human preadipocytes and that these two types of ion channels participate in regulating proliferation of human preadipocytes. © 2008 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_HK
dc.relation.ispartofJournal of Cellular Physiologyen_HK
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleCharacterization of ion channels in human preadipocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0730-2312&volume=218&issue=2&spage=427&epage=435&date=2009&atitle=Characterization+of+ion+channels+in+human+preadipocytes.en_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcp.21617en_HK
dc.identifier.pmid18942098-
dc.identifier.scopuseid_2-s2.0-58149252742en_HK
dc.identifier.hkuros156311en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58149252742&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume218en_HK
dc.identifier.issue2en_HK
dc.identifier.spage427en_HK
dc.identifier.epage435en_HK
dc.identifier.isiWOS:000262270600023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, H=36812244900en_HK
dc.identifier.scopusauthoridHe, ML=7402609178en_HK
dc.identifier.scopusauthoridTao, R=7102857104en_HK
dc.identifier.scopusauthoridSun, HY=35723049200en_HK
dc.identifier.scopusauthoridHu, R=55163131900en_HK
dc.identifier.scopusauthoridZang, WJ=7005740494en_HK
dc.identifier.scopusauthoridYuan, BX=7203056413en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats