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Article: Characterization of ion channels in human preadipocytes
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TitleCharacterization of ion channels in human preadipocytes
 
AuthorsHu, H2 3
He, ML3
Tao, R3
Sun, HY3
Hu, R2 3
Zang, WJ2
Yuan, BX2
Lau, CP3
Tse, HF3
Li, GR3 1
 
Issue Date2009
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
 
CitationJournal Of Cellular Physiology, 2009, v. 218 n. 2, p. 427-435 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21617
 
AbstractIon channels participate in regulation of cell proliferation. However, though preadipocyte (the progenitor of fat cell) is a type of highly proliferating cells, ion channel expression and their role in proliferation is not understood in human preadipocytes. The present study was designed to characterize ion channels using whole-cell patch clamp technique, RT-PCR, and Western blotting. It was found that a 4-aminopyridine- (4-AP) sensitive transient outward K + current (I to) was present in a small population of (32.0%) cells, and an outward "noisy" big conductance Ca 2+-activated K + current (I KCa) was present in most (92.7%) preadipocytes. The noisy current was inhibited by the big conductance I KCa channel blocker paxilline (1 μM), and enhanced by the Ca 2+ ionophore A23187 (5 μM) and the big conductance I KCa channel activator NS1619 (10 μM). RT-PCR and Western blot revealed the molecular identities (i.e., KCa1.1 and Kv4.2) of the functional ionic currents I KCa and I to. Blockade of I KCa or I to with paxilline or 4-AP reduced preadipocyte proliferation, and similar results were obtained with specific siRNAs targeting to KCa1.1 and Kv4.2. Flow cytometric analysis showed ion channel blockade or knockdown of KCa1.1 or Kv4.2 with specific siRNA increased the cell number of G0/G1 phase. The present study demonstrates for the first time that two types of functional ion channel currents, I to and big conductance I KCa, are present in human preadipocytes and that these two types of ion channels participate in regulating proliferation of human preadipocytes. © 2008 Wiley-Liss, Inc.
 
ISSN0021-9541
2012 Impact Factor: 4.218
2012 SCImago Journal Rankings: 1.608
 
DOIhttp://dx.doi.org/10.1002/jcp.21617
 
ISI Accession Number IDWOS:000262270600023
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

The study was supported by a seeding grant from Committee on Research and Conference Grants of University of Hong Kong. The authors thank Mr. Kevin Lai for his excellent technical assistance.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHu, H
 
dc.contributor.authorHe, ML
 
dc.contributor.authorTao, R
 
dc.contributor.authorSun, HY
 
dc.contributor.authorHu, R
 
dc.contributor.authorZang, WJ
 
dc.contributor.authorYuan, BX
 
dc.contributor.authorLau, CP
 
dc.contributor.authorTse, HF
 
dc.contributor.authorLi, GR
 
dc.date.accessioned2010-05-31T03:45:23Z
 
dc.date.available2010-05-31T03:45:23Z
 
dc.date.issued2009
 
dc.description.abstractIon channels participate in regulation of cell proliferation. However, though preadipocyte (the progenitor of fat cell) is a type of highly proliferating cells, ion channel expression and their role in proliferation is not understood in human preadipocytes. The present study was designed to characterize ion channels using whole-cell patch clamp technique, RT-PCR, and Western blotting. It was found that a 4-aminopyridine- (4-AP) sensitive transient outward K + current (I to) was present in a small population of (32.0%) cells, and an outward "noisy" big conductance Ca 2+-activated K + current (I KCa) was present in most (92.7%) preadipocytes. The noisy current was inhibited by the big conductance I KCa channel blocker paxilline (1 μM), and enhanced by the Ca 2+ ionophore A23187 (5 μM) and the big conductance I KCa channel activator NS1619 (10 μM). RT-PCR and Western blot revealed the molecular identities (i.e., KCa1.1 and Kv4.2) of the functional ionic currents I KCa and I to. Blockade of I KCa or I to with paxilline or 4-AP reduced preadipocyte proliferation, and similar results were obtained with specific siRNAs targeting to KCa1.1 and Kv4.2. Flow cytometric analysis showed ion channel blockade or knockdown of KCa1.1 or Kv4.2 with specific siRNA increased the cell number of G0/G1 phase. The present study demonstrates for the first time that two types of functional ion channel currents, I to and big conductance I KCa, are present in human preadipocytes and that these two types of ion channels participate in regulating proliferation of human preadipocytes. © 2008 Wiley-Liss, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Cellular Physiology, 2009, v. 218 n. 2, p. 427-435 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jcp.21617
 
dc.identifier.doihttp://dx.doi.org/10.1002/jcp.21617
 
dc.identifier.epage435
 
dc.identifier.hkuros156311
 
dc.identifier.isiWOS:000262270600023
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

The study was supported by a seeding grant from Committee on Research and Conference Grants of University of Hong Kong. The authors thank Mr. Kevin Lai for his excellent technical assistance.

 
dc.identifier.issn0021-9541
2012 Impact Factor: 4.218
2012 SCImago Journal Rankings: 1.608
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid18942098
 
dc.identifier.scopuseid_2-s2.0-58149252742
 
dc.identifier.spage427
 
dc.identifier.urihttp://hdl.handle.net/10722/59219
 
dc.identifier.volume218
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Cellular Physiology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.
 
dc.titleCharacterization of ion channels in human preadipocytes
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Xi'an Jiaotong University, School of Medicine
  3. The University of Hong Kong