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Article: A microplate compression method for elastic modulus measurement of soft and viscoelastic collagen microspheres

TitleA microplate compression method for elastic modulus measurement of soft and viscoelastic collagen microspheres
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0090-6964
Citation
Annals Of Biomedical Engineering, 2008, v. 36 n. 7, p. 1254-1267 How to Cite?
AbstractHydrogel-based microspheres are commonly used for drug and cell delivery in regenerative medicine. Characterization of their physical and mechanical properties is important in monitoring their quality during fabrication and in predicting their performance upon injection. However, existing methods have limitations in measuring these micron-sized, soft and viscoelastic spherical structures. In this study, a protocol is developed to measure the elastic modulus of non-linear viscoelastic spheres by microplate compression, and is applied to collagen microspheres fabricated with or without cells. During the measurement, a microsphere is placed on a rigid surface and is compressed by a calibrated flexible microplate gripped to a rigid end. A step increase in the displacement rate of the rigid end of the flexible microplate is introduced and the reduced elastic modulus of the microsphere is calculated from the deformation response of the microsphere, using an equation derived in this study. The reduced elastic modulus of collagen microspheres with and without mesenchymal stem cells measured by this method was 9.1 kPa and 132 Pa, respectively. © 2008 Biomedical Engineering Society.
Persistent Identifierhttp://hdl.handle.net/10722/59052
ISSN
2015 Impact Factor: 2.887
2015 SCImago Journal Rankings: 1.182
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, BPen_HK
dc.contributor.authorLi, CHen_HK
dc.contributor.authorAuYeung, KLen_HK
dc.contributor.authorSze, KYen_HK
dc.contributor.authorNgan, AHWen_HK
dc.date.accessioned2010-05-31T03:42:04Z-
dc.date.available2010-05-31T03:42:04Z-
dc.date.issued2008en_HK
dc.identifier.citationAnnals Of Biomedical Engineering, 2008, v. 36 n. 7, p. 1254-1267en_HK
dc.identifier.issn0090-6964en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59052-
dc.description.abstractHydrogel-based microspheres are commonly used for drug and cell delivery in regenerative medicine. Characterization of their physical and mechanical properties is important in monitoring their quality during fabrication and in predicting their performance upon injection. However, existing methods have limitations in measuring these micron-sized, soft and viscoelastic spherical structures. In this study, a protocol is developed to measure the elastic modulus of non-linear viscoelastic spheres by microplate compression, and is applied to collagen microspheres fabricated with or without cells. During the measurement, a microsphere is placed on a rigid surface and is compressed by a calibrated flexible microplate gripped to a rigid end. A step increase in the displacement rate of the rigid end of the flexible microplate is introduced and the reduced elastic modulus of the microsphere is calculated from the deformation response of the microsphere, using an equation derived in this study. The reduced elastic modulus of collagen microspheres with and without mesenchymal stem cells measured by this method was 9.1 kPa and 132 Pa, respectively. © 2008 Biomedical Engineering Society.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0090-6964en_HK
dc.relation.ispartofAnnals of Biomedical Engineeringen_HK
dc.subject.meshCollagen - chemistryen_HK
dc.subject.meshDrug Carriers - chemistryen_HK
dc.subject.meshElasticityen_HK
dc.subject.meshHardness Tests - instrumentation - methodsen_HK
dc.subject.meshHydrogels - chemistryen_HK
dc.subject.meshMaterials Testing - instrumentation - methodsen_HK
dc.subject.meshMicromanipulation - methodsen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshStress, Mechanicalen_HK
dc.subject.meshViscosityen_HK
dc.titleA microplate compression method for elastic modulus measurement of soft and viscoelastic collagen microspheresen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-6964&volume=36&issue=7&spage=1254&epage=1267&date=2008&atitle=A+microplate+compression+method+for+elastic+modulus+measurement+of+soft+and+viscoelastic+collagen+microspheresen_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.emailSze, KY:szeky@graduate.hku.hken_HK
dc.identifier.emailNgan, AHW:hwngan@hkucc.hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.identifier.authoritySze, KY=rp00171en_HK
dc.identifier.authorityNgan, AHW=rp00225en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10439-008-9507-yen_HK
dc.identifier.pmid18454315-
dc.identifier.scopuseid_2-s2.0-44649132624en_HK
dc.identifier.hkuros160757en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-44649132624&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1254en_HK
dc.identifier.epage1267en_HK
dc.identifier.isiWOS:000256318200015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.scopusauthoridLi, CH=24166804000en_HK
dc.identifier.scopusauthoridAuYeung, KL=24166171100en_HK
dc.identifier.scopusauthoridSze, KY=7006735060en_HK
dc.identifier.scopusauthoridNgan, AHW=7006827202en_HK

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