File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Proton magnetic resonance spectroscopy revealed choline reduction in the visual cortex in an experimental model of chronic glaucoma

TitleProton magnetic resonance spectroscopy revealed choline reduction in the visual cortex in an experimental model of chronic glaucoma
Authors
Keywordscholine
chronic glaucoma
intraocular pressure
MRI
proton magnetic resonance spectroscopy
rat
visual cortex
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexer
Citation
Experimental Eye Research, 2009, v. 88 n. 1, p. 65-70 How to Cite?
AbstractGlaucoma is a neurodegenerative disease of the visual system. While elevated intraocular pressure is considered to be a major risk factor, the primary cause and pathogenesis of this disease are still unclear. This study aims to employ in vivo proton magnetic resonance spectroscopy (1H MRS) to evaluate the metabolic changes in the visual cortex in a rat model of chronic glaucoma. Five Sprague-Dawley female rats were prepared to induce ocular hypertension unilaterally in the right eye by photocoagulating the episcleral and limbal veins using an argon laser. Single voxel 1H MRS was performed on each side of the visual cortex 6 weeks after laser treatment. Relative to the creatine level, the choline level was found to be significantly lower in the left glaucomatous visual cortex than the right control visual cortex in all animals. In addition, a marginally significant increase in glutamate level was observed in the glaucomatous visual cortex. No apparent difference was observed between contralateral sides of the visual cortex in T1-weighted or T2-weighted imaging. The results of this study showed that glaucoma is accompanied with alterations in the metabolism of choline-containing compounds in the visual cortex contralateral to the glaucomatous rat eye. These potentially associated the pathophysiological mechanisms of glaucoma with the dysfunction of the cholinergic system in the visual pathway. 1H MRS is a potential tool for studying the metabolic changes in glaucoma in vivo in normally appearing brain structures, and may possess direct clinical applications for humans. Measurement of the Cho:Cr reduction in the visual cortex may be a noninvasive biomarker for this disease. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58826
ISSN
2015 Impact Factor: 2.998
2015 SCImago Journal Rankings: 1.218
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant Council
University of Hong Kong CRCG
Funding Information:

The authors would like to thank Dr. Qing-ling Fu of the Department of Anatomy at The University of Hong Kong for her technical assistance. This work was supported in part by Hong Kong Research Grant Council and The University of Hong Kong CRCG grant.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, KCen_HK
dc.contributor.authorSo, Kfen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2010-05-31T03:37:35Z-
dc.date.available2010-05-31T03:37:35Z-
dc.date.issued2009en_HK
dc.identifier.citationExperimental Eye Research, 2009, v. 88 n. 1, p. 65-70en_HK
dc.identifier.issn0014-4835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58826-
dc.description.abstractGlaucoma is a neurodegenerative disease of the visual system. While elevated intraocular pressure is considered to be a major risk factor, the primary cause and pathogenesis of this disease are still unclear. This study aims to employ in vivo proton magnetic resonance spectroscopy (1H MRS) to evaluate the metabolic changes in the visual cortex in a rat model of chronic glaucoma. Five Sprague-Dawley female rats were prepared to induce ocular hypertension unilaterally in the right eye by photocoagulating the episcleral and limbal veins using an argon laser. Single voxel 1H MRS was performed on each side of the visual cortex 6 weeks after laser treatment. Relative to the creatine level, the choline level was found to be significantly lower in the left glaucomatous visual cortex than the right control visual cortex in all animals. In addition, a marginally significant increase in glutamate level was observed in the glaucomatous visual cortex. No apparent difference was observed between contralateral sides of the visual cortex in T1-weighted or T2-weighted imaging. The results of this study showed that glaucoma is accompanied with alterations in the metabolism of choline-containing compounds in the visual cortex contralateral to the glaucomatous rat eye. These potentially associated the pathophysiological mechanisms of glaucoma with the dysfunction of the cholinergic system in the visual pathway. 1H MRS is a potential tool for studying the metabolic changes in glaucoma in vivo in normally appearing brain structures, and may possess direct clinical applications for humans. Measurement of the Cho:Cr reduction in the visual cortex may be a noninvasive biomarker for this disease. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yexeren_HK
dc.relation.ispartofExperimental Eye Researchen_HK
dc.subjectcholineen_HK
dc.subjectchronic glaucomaen_HK
dc.subjectintraocular pressureen_HK
dc.subjectMRIen_HK
dc.subjectproton magnetic resonance spectroscopyen_HK
dc.subjectraten_HK
dc.subjectvisual cortexen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCholine - metabolismen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGlaucoma, Open-Angle - etiology - metabolismen_HK
dc.subject.meshGlutamic Acid - metabolismen_HK
dc.subject.meshMagnetic Resonance Spectroscopy - methodsen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshVisual Cortex - metabolismen_HK
dc.titleProton magnetic resonance spectroscopy revealed choline reduction in the visual cortex in an experimental model of chronic glaucomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-4835&volume=88&issue=1&spage=65&epage=70&date=2009&atitle=Proton+magnetic+resonance+spectroscopy+revealed+choline+reduction+in+the+visual+cortex+in+an+experimental+model+of+chronic+glaucomaen_HK
dc.identifier.emailSo, Kf:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authoritySo, Kf=rp00329en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.exer.2008.10.002en_HK
dc.identifier.pmid18992243-
dc.identifier.scopuseid_2-s2.0-57549089844en_HK
dc.identifier.hkuros161922en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57549089844&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume88en_HK
dc.identifier.issue1en_HK
dc.identifier.spage65en_HK
dc.identifier.epage70en_HK
dc.identifier.isiWOS:000262395800009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, KC=34968940300en_HK
dc.identifier.scopusauthoridSo, Kf=34668391300en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats