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Article: Characterization of white matter injury in a hypoxic-ischemic neonatal rat model by diffusion tensor MRI

TitleCharacterization of white matter injury in a hypoxic-ischemic neonatal rat model by diffusion tensor MRI
Authors
KeywordsDiffusion tensor imaging
Hypoxic-ischemic
MRI
Neonatal rat
White matter
Issue Date2008
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://stroke.ahajournals.org
Citation
Stroke, 2008, v. 39 n. 8, p. 2348-2353 How to Cite?
AbstractBACKGROUND AND PURPOSE: We evaluate white matter (WM) injury after hypoxic-ischemic (HI) insult in a neonatal rat model using diffusion tensor imaging (DTI) to determine whether λ∥ and λ⊥ are able to characterize type and severity of brain damage. METHODS: Eighteen 7-day-old Sprague-Dawley rats underwent unilateral ligation of left common carotid artery followed by 50 minutes (n≤9) or 90 minutes (n≤9) of hypoxia at 37°C. Rats were divided into 2 groups, according to absence (group A, n≤11) or presence (group B, n≤7) of cystic lesions on D7 post-HI T2-weighted imaging. DTI was performed for all rats at D1 and for group A rats at D7 post-HI. Signal intensity of ipsilateral and contralateral external capsule (EC) on D1 was compared by paired t test, with histological correlation. RESULTS: Group A rats had significantly reduced FA, elevated trace, elevated λ⊥, and similar λ∥ on D1 in the ipsilateral compared to contralateral EC, whereas group B rats had significant reduction in all parameters in the ipsilateral EC. Elevated trace normalized on D7 in group A rats. Histopathologic results demonstrated reduced myelination in group A noncystic HI and severe necrosis in group B cystic HI. CONCLUSIONS: Increased λ⊥ with no significant change in λ appears to characterize noncystic WM injury with reduced myelination, whereas reduction in both λ and λ⊥ characterize severe damage with loss of structural integrity and necrosis. Combining with FA and trace, λ and λ⊥ provide additional information which reflects type and severity of HI injury. © 2008 American Heart Association, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/58811
ISSN
2015 Impact Factor: 5.787
2015 SCImago Journal Rankings: 3.671
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Sen_HK
dc.contributor.authorWu, EXen_HK
dc.contributor.authorTam, CNen_HK
dc.contributor.authorLau, HFen_HK
dc.contributor.authorCheung, PTen_HK
dc.contributor.authorKhong, PLen_HK
dc.date.accessioned2010-05-31T03:37:19Z-
dc.date.available2010-05-31T03:37:19Z-
dc.date.issued2008en_HK
dc.identifier.citationStroke, 2008, v. 39 n. 8, p. 2348-2353en_HK
dc.identifier.issn0039-2499en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58811-
dc.description.abstractBACKGROUND AND PURPOSE: We evaluate white matter (WM) injury after hypoxic-ischemic (HI) insult in a neonatal rat model using diffusion tensor imaging (DTI) to determine whether λ∥ and λ⊥ are able to characterize type and severity of brain damage. METHODS: Eighteen 7-day-old Sprague-Dawley rats underwent unilateral ligation of left common carotid artery followed by 50 minutes (n≤9) or 90 minutes (n≤9) of hypoxia at 37°C. Rats were divided into 2 groups, according to absence (group A, n≤11) or presence (group B, n≤7) of cystic lesions on D7 post-HI T2-weighted imaging. DTI was performed for all rats at D1 and for group A rats at D7 post-HI. Signal intensity of ipsilateral and contralateral external capsule (EC) on D1 was compared by paired t test, with histological correlation. RESULTS: Group A rats had significantly reduced FA, elevated trace, elevated λ⊥, and similar λ∥ on D1 in the ipsilateral compared to contralateral EC, whereas group B rats had significant reduction in all parameters in the ipsilateral EC. Elevated trace normalized on D7 in group A rats. Histopathologic results demonstrated reduced myelination in group A noncystic HI and severe necrosis in group B cystic HI. CONCLUSIONS: Increased λ⊥ with no significant change in λ appears to characterize noncystic WM injury with reduced myelination, whereas reduction in both λ and λ⊥ characterize severe damage with loss of structural integrity and necrosis. Combining with FA and trace, λ and λ⊥ provide additional information which reflects type and severity of HI injury. © 2008 American Heart Association, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://stroke.ahajournals.orgen_HK
dc.relation.ispartofStrokeen_HK
dc.subjectDiffusion tensor imagingen_HK
dc.subjectHypoxic-ischemicen_HK
dc.subjectMRIen_HK
dc.subjectNeonatal raten_HK
dc.subjectWhite matteren_HK
dc.subject.meshAnimals-
dc.subject.meshDiffusion Magnetic Resonance Imaging - methods - statistics and numerical data-
dc.subject.meshHypoxia-Ischemia, Brain - pathology-
dc.subject.meshNerve Fibers, Myelinated - pathology-
dc.subject.meshStroke - pathology-
dc.titleCharacterization of white matter injury in a hypoxic-ischemic neonatal rat model by diffusion tensor MRIen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0039-2499&volume=39&issue=8&spage=2348&epage=2353&date=2008&atitle=Characterization+of+white+matter+injury+in+a+hypoxic-ischemic+neonatal+rat+model+by+diffusion+tensor+MRIen_HK
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.emailCheung, PT:ptcheung@hkucc.hku.hken_HK
dc.identifier.emailKhong, PL:plkhong@hkucc.hku.hken_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.identifier.authorityCheung, PT=rp00351en_HK
dc.identifier.authorityKhong, PL=rp00467en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1161/STROKEAHA.107.509927en_HK
dc.identifier.pmid18535275en_HK
dc.identifier.scopuseid_2-s2.0-49849098275en_HK
dc.identifier.hkuros161961en_HK
dc.identifier.hkuros141498-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49849098275&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2348en_HK
dc.identifier.epage2353en_HK
dc.identifier.isiWOS:000257993400025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, S=24598284300en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.scopusauthoridTam, CN=24597760400en_HK
dc.identifier.scopusauthoridLau, HF=23004851000en_HK
dc.identifier.scopusauthoridCheung, PT=7202595465en_HK
dc.identifier.scopusauthoridKhong, PL=7006693233en_HK

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