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Article: Fibroblast growth factor receptor 2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinoma

TitleFibroblast growth factor receptor 2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinoma
Authors
Issue Date2009
PublisherAmerican Association for Cancer Research
Citation
Clinical Cancer Research, 2009, v. 15 n. 12, p. 4017-4027 How to Cite?
AbstractPurpose: Tumor fibroblasts (TF) have been suggested to play an essential role in the complex process of tumor-stroma interactions and tumorigenesis. The aim of the present study was to investigate the specific role of TF in the esophageal cancer microenvironment. Experimental Design: An Affymetrix expression microarray was used to compare gene expression profiles between six pairs of TFs and normal fibroblasts from esophageal squamous cell carcinoma (ESCC). Differentially expressed genes were identified, and a subset was evaluated by quantitative real-time PCR and immunohistochemistry. Results: About 43% (126 of 292) of known deregulated genes in TFs were associated with cell proliferation, extracellular matrix remodeling, and immune response. Up-regulation of fibroblast growth factor receptor 2 (FGFR2), which showed the most significant change, was detected in all six tested TFs compared with their paired normal fibroblasts. A further study found that FGFR2-positive fibroblasts were only observed inside the tumor tissues and not in tumor-surrounding stromal tissues, suggesting that FGFR2 could be used as a TF-specific marker in ESCC. Moreover, the conditioned medium from TFs was found to be able to promote ESCC tumor cell growth, migration, and invasion in vitro. Conclusions: Ourstudy provides new candidate genes forthe esophageal cancermicroenvironment. Based on our results, we hypothesize that FGFR2(+)-TFs might provide cancer cells with a suitable microenvironment via secretion of proteins that could promote cancer development and progression through stimulation of cancer cell proliferation, induction of angiogenesis, inhibition of cell adhesion, enhancement of cell mobility, and promotion of the epithelial-mesenchymal transition. © 2009 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/58609
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30700462
30772475
Major State Basic Research Program of China2006CB910104
China Postdoctoral Science Foundation20070410861
Sun Yat-Sen University85000-3171311
RGCHKU 7656/07M
HKUST2/06C
Funding Information:

Grant support: National Natural Science Foundation of China grants 30700462 and 30772475, Major State Basic Research Program of China grant 2006CB910104, China Postdoctoral Science Foundation grant 20070410861, Sun Yat-Sen University "Hundred Talents Program" grant 85000-3171311, and RGC grants HKU 7656/07M and HKUST2/06C.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorFu, Len_HK
dc.contributor.authorFu, Jen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorYang, Hen_HK
dc.contributor.authorRong, THen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorFu, SBen_HK
dc.contributor.authorZeng, YXen_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-05-31T03:33:27Z-
dc.date.available2010-05-31T03:33:27Z-
dc.date.issued2009en_HK
dc.identifier.citationClinical Cancer Research, 2009, v. 15 n. 12, p. 4017-4027en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58609-
dc.description.abstractPurpose: Tumor fibroblasts (TF) have been suggested to play an essential role in the complex process of tumor-stroma interactions and tumorigenesis. The aim of the present study was to investigate the specific role of TF in the esophageal cancer microenvironment. Experimental Design: An Affymetrix expression microarray was used to compare gene expression profiles between six pairs of TFs and normal fibroblasts from esophageal squamous cell carcinoma (ESCC). Differentially expressed genes were identified, and a subset was evaluated by quantitative real-time PCR and immunohistochemistry. Results: About 43% (126 of 292) of known deregulated genes in TFs were associated with cell proliferation, extracellular matrix remodeling, and immune response. Up-regulation of fibroblast growth factor receptor 2 (FGFR2), which showed the most significant change, was detected in all six tested TFs compared with their paired normal fibroblasts. A further study found that FGFR2-positive fibroblasts were only observed inside the tumor tissues and not in tumor-surrounding stromal tissues, suggesting that FGFR2 could be used as a TF-specific marker in ESCC. Moreover, the conditioned medium from TFs was found to be able to promote ESCC tumor cell growth, migration, and invasion in vitro. Conclusions: Ourstudy provides new candidate genes forthe esophageal cancermicroenvironment. Based on our results, we hypothesize that FGFR2(+)-TFs might provide cancer cells with a suitable microenvironment via secretion of proteins that could promote cancer development and progression through stimulation of cancer cell proliferation, induction of angiogenesis, inhibition of cell adhesion, enhancement of cell mobility, and promotion of the epithelial-mesenchymal transition. © 2009 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Researchen_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshCarcinoma - enzymology - genetics - pathologyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Movement - drug effectsen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCulture Media, Conditioned - pharmacologyen_HK
dc.subject.meshEsophageal Neoplasms - enzymology - genetics - pathologyen_HK
dc.subject.meshFibroblasts - drug effects - enzymology - pathologyen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshReceptor, Fibroblast Growth Factor, Type 2 - metabolismen_HK
dc.subject.meshUp-Regulation - genetics - physiologyen_HK
dc.titleFibroblast growth factor receptor 2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=15&spage=4017&epage=4027&date=2009&atitle=Fibroblast+growth+factor+receptor+2-positive+fibroblasts+provide+a+suitable+microenvironment+for+tumor+development+and+progression+in+esophageal+carcinoma.en_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/1078-0432.CCR-08-2824en_HK
dc.identifier.pmid19509166-
dc.identifier.scopuseid_2-s2.0-67449164585en_HK
dc.identifier.hkuros157420en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67449164585&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue12en_HK
dc.identifier.spage4017en_HK
dc.identifier.epage4027en_HK
dc.identifier.isiWOS:000267080800016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, C=14033447100en_HK
dc.identifier.scopusauthoridFu, L=12238958200en_HK
dc.identifier.scopusauthoridFu, J=8228815900en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridYang, H=7406561423en_HK
dc.identifier.scopusauthoridRong, TH=7006120612en_HK
dc.identifier.scopusauthoridLi, Y=36078824800en_HK
dc.identifier.scopusauthoridLiu, H=27171509500en_HK
dc.identifier.scopusauthoridFu, SB=7402732420en_HK
dc.identifier.scopusauthoridZeng, YX=7402981579en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK

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