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Article: Characterization and quantification of class 1 integrons and associated gene cassettes in sewage treatment plants

TitleCharacterization and quantification of class 1 integrons and associated gene cassettes in sewage treatment plants
Authors
Issue Date2009
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology And Biotechnology, 2009, v. 82 n. 6, p. 1169-1177 How to Cite?
AbstractClass 1 integrons and gene cassettes containing antibiotic resistance genes (ARGs) in five different sewage treatment plants (STPs) were characterized and quantified using polymerase chain reaction (PCR), sequencing, and quantitative real-time PCR (qRT-PCR) in this study. Class 1 integronase gene (intI1) was found commonly occurring in all of activated sludge samples from the five STPs, as well as in influent and effluent of two STPs at Hong Kong. One hundred and nine lactose-fermenting Enterobacteriaceae (LFE) strains were isolated from activated sludge of Shatin STP. Among them, 36 strains (33.0%) were found to carry class 1 integrons. PCR assays showed that 11 of the 36 intI1-carrying isolates harbored a common type of gene cassette array of about 1,600 bps, as well as the static genes (sulI and qacEΔ1) on class 1 integrons. This gene cassette array was found phylogenetically close to antibiotic resistance genes dfr17 and aadA5, encoding dihydrofolate reductase conferring resistance to trimethoprim and adenylyltransferase conferring resistance to spectinomycin/streptomycin, respectively. Antimicrobial susceptibility analysis demonstrated that all the 11 LFEs carrying gene cassette were multi-resistant, especially having common resistance to trimethoprim and streptomycin. qRT-PCR assay showed that genes copies of both class 1 integron and the gene cassette varied significantly among the activated sludge sampled from different STPs, at different time points or different treatment steps. More than 90% of class 1 integrons and the gene cassette were removed by activated sludge processes in two STPs, while the disinfection process removed 94% integron and 77% gene cassette in one STP. © 2009 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/58554
ISSN
2015 Impact Factor: 3.376
2015 SCImago Journal Rankings: 1.262
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7195/06E
Funding Information:

The authors wish to thank the Hong Kong Research Grants Council for the financial support of this study (HKU 7195/06E).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorZhang, XXen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorZhang, Men_HK
dc.contributor.authorFang, HHPen_HK
dc.contributor.authorCheng, SPen_HK
dc.date.accessioned2010-05-31T03:32:26Z-
dc.date.available2010-05-31T03:32:26Z-
dc.date.issued2009en_HK
dc.identifier.citationApplied Microbiology And Biotechnology, 2009, v. 82 n. 6, p. 1169-1177en_HK
dc.identifier.issn0175-7598en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58554-
dc.description.abstractClass 1 integrons and gene cassettes containing antibiotic resistance genes (ARGs) in five different sewage treatment plants (STPs) were characterized and quantified using polymerase chain reaction (PCR), sequencing, and quantitative real-time PCR (qRT-PCR) in this study. Class 1 integronase gene (intI1) was found commonly occurring in all of activated sludge samples from the five STPs, as well as in influent and effluent of two STPs at Hong Kong. One hundred and nine lactose-fermenting Enterobacteriaceae (LFE) strains were isolated from activated sludge of Shatin STP. Among them, 36 strains (33.0%) were found to carry class 1 integrons. PCR assays showed that 11 of the 36 intI1-carrying isolates harbored a common type of gene cassette array of about 1,600 bps, as well as the static genes (sulI and qacEΔ1) on class 1 integrons. This gene cassette array was found phylogenetically close to antibiotic resistance genes dfr17 and aadA5, encoding dihydrofolate reductase conferring resistance to trimethoprim and adenylyltransferase conferring resistance to spectinomycin/streptomycin, respectively. Antimicrobial susceptibility analysis demonstrated that all the 11 LFEs carrying gene cassette were multi-resistant, especially having common resistance to trimethoprim and streptomycin. qRT-PCR assay showed that genes copies of both class 1 integron and the gene cassette varied significantly among the activated sludge sampled from different STPs, at different time points or different treatment steps. More than 90% of class 1 integrons and the gene cassette were removed by activated sludge processes in two STPs, while the disinfection process removed 94% integron and 77% gene cassette in one STP. © 2009 Springer-Verlag.en_HK
dc.languageengen_HK
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htmen_HK
dc.relation.ispartofApplied Microbiology and Biotechnologyen_HK
dc.subject.meshAnti-Bacterial Agents - pharmacologyen_HK
dc.subject.meshDNA, Bacterial - chemistry - genetics - isolation & purificationen_HK
dc.subject.meshDrug Resistance, Bacterialen_HK
dc.subject.meshEnterobacteriaceae - genetics - isolation & purificationen_HK
dc.subject.meshGene Orderen_HK
dc.subject.meshGenes, Bacterialen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshIntegronsen_HK
dc.subject.meshMicrobial Sensitivity Testsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSequence Homologyen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.subject.meshWater Purificationen_HK
dc.titleCharacterization and quantification of class 1 integrons and associated gene cassettes in sewage treatment plantsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0175-7598&volume= 82 &spage=1169&epage=77 &date=2009&atitle=Characterization+and+quantification+of+class+1+integrons+and+associated+gene+cassettes+in+sewage+treatment+plantsen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00253-009-1886-yen_HK
dc.identifier.pmid19224208en_HK
dc.identifier.scopuseid_2-s2.0-63849344975en_HK
dc.identifier.hkuros155506en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63849344975&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume82en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1169en_HK
dc.identifier.epage1177en_HK
dc.identifier.isiWOS:000265382200018-
dc.publisher.placeGermanyen_HK
dc.relation.projectMicrobial diversity and characteristics of activated sludge treating Hong Kong's saline sewage-
dc.identifier.scopusauthoridZhang, XX=35239161000en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridZhang, M=36112530200en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.scopusauthoridCheng, SP=18039405400en_HK
dc.identifier.citeulike4082273-

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