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Article: Tetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plants

TitleTetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plants
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2009, v. 43 n. 10, p. 3455-3460 How to Cite?
AbstractActivated sludges were sampled from five sewage treatment plants (STPs) distributed in three geographically isolated areas, i.e., Hong Kong (Shatin, Stanley), Shanghai (Minhang) in China, and the bay area in California (Palo Alto and San Jose) of the United States. Among the tested 14 tetracycline resistance (tet) genes, nine genes encompassing efflux pumps (tetA, tetC, tetE, and tetG), ribosomal protection proteins (tetM, tetO, tetQ, and tetS), and enzymatic modification (tetX) were commonly detected in the STP sludge samples, whereas five genes encompassing efflux pumps [tetB, tetD, tetL, tetK, and tetA(P)] were not detected in any sludge sample. Additionally, 109 lactose-fermenting Enterobacteriaceae (LFE) strains were isolated from the activated sludge of the Shatin STP. Tetracycline-resistant (TR) LFE accounted for 32% of the total 109 LFE strains. The occurrence frequencies of tet genes among all TR-LEF strains varied from 0 to 91%, i.e., tetC (91%), tetA (46%), tetE (9%), tetG (6%), and tetD (6%). Finally, quantitative real-time polymerase chain reaction was used to quantify the change of tetC and tetA genes as the indicator of TR-LEF in the Shatin and Stanley STPs. The results showed that the concentrations of tetC and tetA genes in STP effluent ranged from 104 to 105 copies/mL, significantly lower than those in the influent by 3 orders of magnitude. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/58549
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
Funding AgencyGrant Number
General Research Fund (GRF) of Hong Kong7195/06E
University of Hong Kong (HKU)
Funding Information:

This study was partially supported by the General Research Fund (GRF) of Hong Kong (7195/06E). M. Zhang and X. Zhang thank The University of Hong Kong (HKU) for the fellowship. We all thank Prof. Craig Criddle and Mr. George Wells of Stanford University for the DNA samples from the two STPs in the United States.

References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorZhang, Men_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorFang, HHen_HK
dc.date.accessioned2010-05-31T03:32:21Z-
dc.date.available2010-05-31T03:32:21Z-
dc.date.issued2009en_HK
dc.identifier.citationEnvironmental Science And Technology, 2009, v. 43 n. 10, p. 3455-3460en_HK
dc.identifier.issn0013-936Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/58549-
dc.description.abstractActivated sludges were sampled from five sewage treatment plants (STPs) distributed in three geographically isolated areas, i.e., Hong Kong (Shatin, Stanley), Shanghai (Minhang) in China, and the bay area in California (Palo Alto and San Jose) of the United States. Among the tested 14 tetracycline resistance (tet) genes, nine genes encompassing efflux pumps (tetA, tetC, tetE, and tetG), ribosomal protection proteins (tetM, tetO, tetQ, and tetS), and enzymatic modification (tetX) were commonly detected in the STP sludge samples, whereas five genes encompassing efflux pumps [tetB, tetD, tetL, tetK, and tetA(P)] were not detected in any sludge sample. Additionally, 109 lactose-fermenting Enterobacteriaceae (LFE) strains were isolated from the activated sludge of the Shatin STP. Tetracycline-resistant (TR) LFE accounted for 32% of the total 109 LFE strains. The occurrence frequencies of tet genes among all TR-LEF strains varied from 0 to 91%, i.e., tetC (91%), tetA (46%), tetE (9%), tetG (6%), and tetD (6%). Finally, quantitative real-time polymerase chain reaction was used to quantify the change of tetC and tetA genes as the indicator of TR-LEF in the Shatin and Stanley STPs. The results showed that the concentrations of tetC and tetA genes in STP effluent ranged from 104 to 105 copies/mL, significantly lower than those in the influent by 3 orders of magnitude. © 2009 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_HK
dc.relation.ispartofEnvironmental Science and Technologyen_HK
dc.subject.meshEnterobacteriaceae - genetics-
dc.subject.meshFermentation - genetics-
dc.subject.meshSewage - microbiology-
dc.subject.meshTetracycline Resistance - genetics-
dc.subject.meshWater Purification-
dc.titleTetracycline resistance genes and tetracycline resistant lactose-fermenting Enterobacteriaceae in activated sludge of sewage treatment plantsen_HK
dc.typeArticleen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HH:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HH=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/es803309men_HK
dc.identifier.pmid19544839-
dc.identifier.scopuseid_2-s2.0-66249095675en_HK
dc.identifier.hkuros161877en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66249095675&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3455en_HK
dc.identifier.epage3460en_HK
dc.identifier.eissn1520-5851-
dc.identifier.isiWOS:000266046700012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridZhang, M=7601555746en_HK
dc.identifier.scopusauthoridZhang, X=8555121100en_HK
dc.identifier.scopusauthoridFang, HH=7402542625en_HK

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