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Article: Rapid analysis of 21 antibiotics of multiple classes in municipal wastewater using ultra performance liquid chromatography-tandem mass spectrometry

TitleRapid analysis of 21 antibiotics of multiple classes in municipal wastewater using ultra performance liquid chromatography-tandem mass spectrometry
Authors
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aca
Citation
Analytica Chimica Acta, 2009, v. 645 n. 1-2, p. 64-72 How to Cite?
AbstractIn this study, solid phase extraction (SPE) and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to develop a rapid, sensitive and reliable method for trace analysis of 21 antibiotics belonging to 7 classes in influent and effluent of municipal wastewater treatment plant. Detection parameters for SPE preconcentration and UPLC-MS/MS analysis were optimized, including sample pH, eluant, mobile phase (solvent and formic acid concentration), column temperature, and flow rate. Under the optimal conditions, the recoveries for different antibiotics ranged from 60.5% to 109.7% and all analytes were detected within 10.0 min by UPLC-MS/MS. The validation study indicated that the method detection limits (MDLs) for the 21 antibiotics were from 0.3 to 60.0 ng L-1 for effluent, while higher MDLs (0.5-84.0 ng L-1) for influent. Accuracy and precision for both within-run (-11.6 to 14.3% and 0.2 to 15.6%) and between-run tests (-7.2 to 8.7% and 1.9 to 16.4%) were acceptable. The analysis of influent and effluent samples of two municipal wastewater treatment plants in Hong Kong revealed the presence of 11 antibiotics, including ampicillin, cefalexin, sulfamethoxazole, sulfadiazine, norfloxacin, ciprofloxacin, ofloxacin, tetracycline, roxithromycin, erythromycin-H2O, and trimethoprim. Their concentration ranged from 3.5 to 720.0 ng L-1 in influent and from 2.1 to 556.4 ng L-1 in effluent. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58526
ISSN
2015 Impact Factor: 4.712
2015 SCImago Journal Rankings: 1.548
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7195/06E
Funding Information:

The authors wish to thank Dr. Xiangdong Li (The Hong Kong Polytechnic University), Dr. Gan Zhang (Guangzhou Institute of Geochemistry, Chinese Academy of Science), Dr. Weihai Xu (Guangzhou Institute of Geochemistry, Chinese Academy of Science), Dr. Stephanie Lee (Waters Ltd.) and Xiuli Wang (Waters Ltd.) for their technical support to start this study. The authors wish to thank the Hong Kong Research Grants Council for the financial support of this study (HKU 7195/06E).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLi, Ben_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorXu, Zen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-05-31T03:31:58Z-
dc.date.available2010-05-31T03:31:58Z-
dc.date.issued2009en_HK
dc.identifier.citationAnalytica Chimica Acta, 2009, v. 645 n. 1-2, p. 64-72en_HK
dc.identifier.issn0003-2670en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58526-
dc.description.abstractIn this study, solid phase extraction (SPE) and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) were utilized to develop a rapid, sensitive and reliable method for trace analysis of 21 antibiotics belonging to 7 classes in influent and effluent of municipal wastewater treatment plant. Detection parameters for SPE preconcentration and UPLC-MS/MS analysis were optimized, including sample pH, eluant, mobile phase (solvent and formic acid concentration), column temperature, and flow rate. Under the optimal conditions, the recoveries for different antibiotics ranged from 60.5% to 109.7% and all analytes were detected within 10.0 min by UPLC-MS/MS. The validation study indicated that the method detection limits (MDLs) for the 21 antibiotics were from 0.3 to 60.0 ng L-1 for effluent, while higher MDLs (0.5-84.0 ng L-1) for influent. Accuracy and precision for both within-run (-11.6 to 14.3% and 0.2 to 15.6%) and between-run tests (-7.2 to 8.7% and 1.9 to 16.4%) were acceptable. The analysis of influent and effluent samples of two municipal wastewater treatment plants in Hong Kong revealed the presence of 11 antibiotics, including ampicillin, cefalexin, sulfamethoxazole, sulfadiazine, norfloxacin, ciprofloxacin, ofloxacin, tetracycline, roxithromycin, erythromycin-H2O, and trimethoprim. Their concentration ranged from 3.5 to 720.0 ng L-1 in influent and from 2.1 to 556.4 ng L-1 in effluent. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/acaen_HK
dc.relation.ispartofAnalytica Chimica Actaen_HK
dc.rightsAnalytica Chimica Acta. Copyright © Elsevier BV.-
dc.subject.meshAnti-Bacterial Agents - analysisen_HK
dc.subject.meshChromatography, Liquid - methodsen_HK
dc.subject.meshFormic Acids - chemistryen_HK
dc.subject.meshHong Kongen_HK
dc.subject.meshHydrogen-Ion Concentrationen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSolid Phase Extraction - methodsen_HK
dc.subject.meshTandem Mass Spectrometry - methodsen_HK
dc.subject.meshTemperatureen_HK
dc.subject.meshWaste Disposal, Fluiden_HK
dc.subject.meshWater - analysisen_HK
dc.subject.meshWater Pollutants, Chemical - analysisen_HK
dc.titleRapid analysis of 21 antibiotics of multiple classes in municipal wastewater using ultra performance liquid chromatography-tandem mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2670&volume=645&issue=1-2&spage=64&epage=72&date=2009&atitle=Rapid+analysis+of+21+antibiotics+of+multiple+classes+in+municipal+wastewater+using+ultra+performance+liquid+chromatography-tandem+mass+spectrometryen_HK
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.aca.2009.04.042en_HK
dc.identifier.pmid19481632-
dc.identifier.scopuseid_2-s2.0-66049130018en_HK
dc.identifier.hkuros161878en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66049130018&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume645en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage64en_HK
dc.identifier.epage72en_HK
dc.identifier.eissn1873-4324-
dc.identifier.isiWOS:000266952100010-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectMicrobial diversity and characteristics of activated sludge treating Hong Kong's saline sewage-
dc.identifier.scopusauthoridLi, B=36072052100en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridXu, Z=7405427929en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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