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Article: Functional characterization of key structural genes in rice flavonoid biosynthesis

TitleFunctional characterization of key structural genes in rice flavonoid biosynthesis
Authors
KeywordsAnthocyanin
Flavones
Flavonoid structural genes
Rice
Issue Date2008
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2008, v. 228 n. 6, p. 1043-1054 How to Cite?
AbstractRice is a model system for monocot but the molecular features of rice flavonoid biosynthesis have not been extensively characterized. Rice structural gene homologs encoding chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) were identified by homology searches. Unique differential expression of OsF3H, OsDFR, and OsANS1 controlled by the Pl w locus, which contains the R/B-type regulatory genes OSB1 and OSB2, was demonstrated during light-induced anthocyanin accumulation in T65-Plw seedlings. Previously, F3H genes were often considered as early genes co-regulated with CHS and CHI genes in other plants. In selected non-pigmented rice lines, OSB2 is not expressed following illumination while their expressed OSB1sequences all contain the same nucleotide change leading to the T 64 M substitution within the conserved N-terminal interacting domain. Furthermore, the biochemical roles of the expressed rice structural genes (OsCHS1, OsCHI, OsF3H, and OsF3′H) were established in planta for the first time by complementation in the appropriate Arabidopsis transparent testa mutants. Using yeast two-hybrid analysis, OsCHS1 was demonstrated to interact physically with OsF3H, OsF3′H, OsDFR, and OsANS1, suggesting the existence of a macromolecular complex for anthocyanin biosynthesis in rice. Finally, flavones were identified as the major flavonoid class in the non-pigmented T65 seedlings in which the single-copy OsF3H gene was not expressed. Competition between flavone and anthocyanin pathways was evidenced by the significant reduction of tricin accumulation in the T65-Plw seedlings. © 2008 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/58463
ISSN
2015 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.470
ISI Accession Number ID
Funding AgencyGrant Number
Council of the Hong Kong Special Administrative Region, ChinaHKU 7337/04M
Funding Information:

We thank Prof S Iida of National Institute for Basic Biology (Okazaki, Japan) for the OsDFR cDNA clone and the National Institute of Agrobiological Sciences (Tsukuba, Japan) for the OsCHS1, OsCHI, and OsF3' H clones. This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (grant no. HKU 7337/04M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorShih, CHen_HK
dc.contributor.authorChu, Hen_HK
dc.contributor.authorTang, LKen_HK
dc.contributor.authorSakamoto, Wen_HK
dc.contributor.authorMaekawa, Men_HK
dc.contributor.authorChu, IKen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorLo, Cen_HK
dc.date.accessioned2010-05-31T03:30:45Z-
dc.date.available2010-05-31T03:30:45Z-
dc.date.issued2008en_HK
dc.identifier.citationPlanta, 2008, v. 228 n. 6, p. 1043-1054en_HK
dc.identifier.issn0032-0935en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58463-
dc.description.abstractRice is a model system for monocot but the molecular features of rice flavonoid biosynthesis have not been extensively characterized. Rice structural gene homologs encoding chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) were identified by homology searches. Unique differential expression of OsF3H, OsDFR, and OsANS1 controlled by the Pl w locus, which contains the R/B-type regulatory genes OSB1 and OSB2, was demonstrated during light-induced anthocyanin accumulation in T65-Plw seedlings. Previously, F3H genes were often considered as early genes co-regulated with CHS and CHI genes in other plants. In selected non-pigmented rice lines, OSB2 is not expressed following illumination while their expressed OSB1sequences all contain the same nucleotide change leading to the T 64 M substitution within the conserved N-terminal interacting domain. Furthermore, the biochemical roles of the expressed rice structural genes (OsCHS1, OsCHI, OsF3H, and OsF3′H) were established in planta for the first time by complementation in the appropriate Arabidopsis transparent testa mutants. Using yeast two-hybrid analysis, OsCHS1 was demonstrated to interact physically with OsF3H, OsF3′H, OsDFR, and OsANS1, suggesting the existence of a macromolecular complex for anthocyanin biosynthesis in rice. Finally, flavones were identified as the major flavonoid class in the non-pigmented T65 seedlings in which the single-copy OsF3H gene was not expressed. Competition between flavone and anthocyanin pathways was evidenced by the significant reduction of tricin accumulation in the T65-Plw seedlings. © 2008 Springer-Verlag.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425en_HK
dc.relation.ispartofPlantaen_HK
dc.subjectAnthocyaninen_HK
dc.subjectFlavonesen_HK
dc.subjectFlavonoid structural genesen_HK
dc.subjectRiceen_HK
dc.titleFunctional characterization of key structural genes in rice flavonoid biosynthesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0935&volume=228&spage=1043&epage=1054&date=2008&atitle=Functional+characterization+of+key+structural+genes+in+rice+flavonoid+biosynthesisen_HK
dc.identifier.emailChu, IK: ivankchu@hku.hken_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailLo, C: clivelo@hkucc.hku.hken_HK
dc.identifier.authorityChu, IK=rp00683en_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityLo, C=rp00751en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00425-008-0806-1en_HK
dc.identifier.pmid18726614-
dc.identifier.scopuseid_2-s2.0-55849106983en_HK
dc.identifier.hkuros153376en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55849106983&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume228en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1043en_HK
dc.identifier.epage1054en_HK
dc.identifier.isiWOS:000260782800014-
dc.publisher.placeGermanyen_HK
dc.relation.projectFunctional characterization and metabolic engineering of flavonoid pathways in rice-
dc.identifier.scopusauthoridShih, CH=55233136000en_HK
dc.identifier.scopusauthoridChu, H=36870371300en_HK
dc.identifier.scopusauthoridTang, LK=24605748900en_HK
dc.identifier.scopusauthoridSakamoto, W=7101749686en_HK
dc.identifier.scopusauthoridMaekawa, M=35313453100en_HK
dc.identifier.scopusauthoridChu, IK=7103327484en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridLo, C=15737175700en_HK

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