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Article: Nuclear penetration of surface functionalized gold nanoparticles
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TitleNuclear penetration of surface functionalized gold nanoparticles
 
AuthorsGu, YJ2
Cheng, J2
Lin, CC2
Lam, YW2
Cheng, SH2
Wong, WT1
 
KeywordsBiomaterials
Gold nanoparticles
Nuclear penetration
Surface modification
 
Issue Date2009
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
 
CitationToxicology And Applied Pharmacology, 2009, v. 237 n. 2, p. 196-204 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.taap.2009.03.009
 
AbstractFree gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier. © 2009 Elsevier Inc. All rights reserved.
 
ISSN0041-008X
2012 Impact Factor: 3.975
2012 SCImago Journal Rankings: 1.328
 
DOIhttp://dx.doi.org/10.1016/j.taap.2009.03.009
 
ISI Accession Number IDWOS:000266289200008
Funding AgencyGrant Number
City University of Hong KongCityU 160108
City University of Hong Kong Research Enhancement Scheme
Hong Kong Research Grants Council
University of Hong Kong
Funding Information:

The work described in this paper was totally supported by a grant from the City University of Hong Kong (Project No. CityU 160108). Dr. Gu acknowledges the receipt of post-doctoral fellowship from the City University of Hong Kong Research Enhancement Scheme for their financial support. We also acknowledge the Hong Kong Research Grants Council and The University of Hong Kong for financial support.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorGu, YJ
 
dc.contributor.authorCheng, J
 
dc.contributor.authorLin, CC
 
dc.contributor.authorLam, YW
 
dc.contributor.authorCheng, SH
 
dc.contributor.authorWong, WT
 
dc.date.accessioned2010-05-31T03:30:32Z
 
dc.date.available2010-05-31T03:30:32Z
 
dc.date.issued2009
 
dc.description.abstractFree gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier. © 2009 Elsevier Inc. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationToxicology And Applied Pharmacology, 2009, v. 237 n. 2, p. 196-204 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.taap.2009.03.009
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.taap.2009.03.009
 
dc.identifier.epage204
 
dc.identifier.hkuros156628
 
dc.identifier.isiWOS:000266289200008
Funding AgencyGrant Number
City University of Hong KongCityU 160108
City University of Hong Kong Research Enhancement Scheme
Hong Kong Research Grants Council
University of Hong Kong
Funding Information:

The work described in this paper was totally supported by a grant from the City University of Hong Kong (Project No. CityU 160108). Dr. Gu acknowledges the receipt of post-doctoral fellowship from the City University of Hong Kong Research Enhancement Scheme for their financial support. We also acknowledge the Hong Kong Research Grants Council and The University of Hong Kong for financial support.

 
dc.identifier.issn0041-008X
2012 Impact Factor: 3.975
2012 SCImago Journal Rankings: 1.328
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid19328820
 
dc.identifier.scopuseid_2-s2.0-67349146754
 
dc.identifier.spage196
 
dc.identifier.urihttp://hdl.handle.net/10722/58451
 
dc.identifier.volume237
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
 
dc.publisher.placeUnited States
 
dc.relation.ispartofToxicology and Applied Pharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectBiomaterials
 
dc.subjectGold nanoparticles
 
dc.subjectNuclear penetration
 
dc.subjectSurface modification
 
dc.titleNuclear penetration of surface functionalized gold nanoparticles
 
dc.typeArticle
 
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<contributor.author>Cheng, SH</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. City University of Hong Kong