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Article: Nuclear penetration of surface functionalized gold nanoparticles

TitleNuclear penetration of surface functionalized gold nanoparticles
Authors
KeywordsBiomaterials
Gold nanoparticles
Nuclear penetration
Surface modification
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taap
Citation
Toxicology And Applied Pharmacology, 2009, v. 237 n. 2, p. 196-204 How to Cite?
AbstractFree gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58451
ISSN
2014 Impact Factor: 3.705
2014 SCImago Journal Rankings: 1.220
ISI Accession Number ID
Funding AgencyGrant Number
City University of Hong KongCityU 160108
City University of Hong Kong Research Enhancement Scheme
Hong Kong Research Grants Council
University of Hong Kong
Funding Information:

The work described in this paper was totally supported by a grant from the City University of Hong Kong (Project No. CityU 160108). Dr. Gu acknowledges the receipt of post-doctoral fellowship from the City University of Hong Kong Research Enhancement Scheme for their financial support. We also acknowledge the Hong Kong Research Grants Council and The University of Hong Kong for financial support.

References

 

DC FieldValueLanguage
dc.contributor.authorGu, YJen_HK
dc.contributor.authorCheng, Jen_HK
dc.contributor.authorLin, CCen_HK
dc.contributor.authorLam, YWen_HK
dc.contributor.authorCheng, SHen_HK
dc.contributor.authorWong, WTen_HK
dc.date.accessioned2010-05-31T03:30:32Z-
dc.date.available2010-05-31T03:30:32Z-
dc.date.issued2009en_HK
dc.identifier.citationToxicology And Applied Pharmacology, 2009, v. 237 n. 2, p. 196-204en_HK
dc.identifier.issn0041-008Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/58451-
dc.description.abstractFree gold nanoparticles easily aggregate when the environment conditions change. Here, gold nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modified with poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were first surface-modified with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end groups on PEG and the carboxylic acid groups on the particles. The biocompatibility and intracellular fate of PEG-modified gold nanoparticles (AuNP@MPA-PEG) were then studied in human cervical cancer (HeLa) cells. Cell viability test showed that AuNP@MPA-PEG did not induce obvious cytotoxicity. Both confocal laser scanning microscopy and transmission electron microscopy demonstrated that AuNP@MPA-PEG entered into mammalian cells and the cellular uptake of AuNP@MPA-PEG was time-dependent. Inductively coupled plasma mass spectrometry and confocal microscopy imaging further demonstrated that AuNP@MPA-PEG penetrated into the nucleus of mammalian cells upon exposure for 24 h. These results suggest that surface modification can enhance the stability and improve the biocompatibility. This study also indicates that AuNP@MPA-PEG can be used as potential nuclear targeted drug delivery carrier. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/taapen_HK
dc.relation.ispartofToxicology and Applied Pharmacologyen_HK
dc.subjectBiomaterialsen_HK
dc.subjectGold nanoparticlesen_HK
dc.subjectNuclear penetrationen_HK
dc.subjectSurface modificationen_HK
dc.titleNuclear penetration of surface functionalized gold nanoparticlesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0041-008X&volume=237&spage=196&epage=204&date=2009&atitle=Nuclear+Penetration+of+Surface+Functionalized+Gold+Nanoparticles+en_HK
dc.identifier.emailWong, WT: wtwong@hku.hken_HK
dc.identifier.authorityWong, WT=rp00811en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.taap.2009.03.009en_HK
dc.identifier.pmid19328820en_HK
dc.identifier.scopuseid_2-s2.0-67349146754en_HK
dc.identifier.hkuros156628en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349146754&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume237en_HK
dc.identifier.issue2en_HK
dc.identifier.spage196en_HK
dc.identifier.epage204en_HK
dc.identifier.isiWOS:000266289200008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGu, YJ=14319095800en_HK
dc.identifier.scopusauthoridCheng, J=18234601200en_HK
dc.identifier.scopusauthoridLin, CC=12789042300en_HK
dc.identifier.scopusauthoridLam, YW=7202563947en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.scopusauthoridWong, WT=7403973084en_HK

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