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Article: Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes

TitlePhosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes
Authors
KeywordsDNA cleavage
Guanidinium
Inhibition
Phosphate diester
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbio
Citation
Journal Of Inorganic Biochemistry, 2009, v. 103 n. 5, p. 851-858 How to Cite?
AbstractTwo new Zn(II) complexes containing guanidinium groups, [Zn(L1)Cl2](ClO4)2 · H2O · CH3OH (1) and [Zn(L2)Cl2](ClO4)2 · 0.5H2O (2), were synthesized and characterized (L1 = 5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L2 = 6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both 1 and 2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl2] (bpy = 2,2′-bipyridy) (3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (DraI and SmaI) by complexes show that 1 and 2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while 3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58351
ISSN
2015 Impact Factor: 3.205
2015 SCImago Journal Rankings: 0.983
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, Jen_HK
dc.contributor.authorSun, Jen_HK
dc.contributor.authorMao, ZWen_HK
dc.contributor.authorJi, LNen_HK
dc.contributor.authorSun, Hen_HK
dc.date.accessioned2010-05-31T03:28:47Z-
dc.date.available2010-05-31T03:28:47Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Inorganic Biochemistry, 2009, v. 103 n. 5, p. 851-858en_HK
dc.identifier.issn0162-0134en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58351-
dc.description.abstractTwo new Zn(II) complexes containing guanidinium groups, [Zn(L1)Cl2](ClO4)2 · H2O · CH3OH (1) and [Zn(L2)Cl2](ClO4)2 · 0.5H2O (2), were synthesized and characterized (L1 = 5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L2 = 6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both 1 and 2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl2] (bpy = 2,2′-bipyridy) (3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (DraI and SmaI) by complexes show that 1 and 2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while 3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbioen_HK
dc.relation.ispartofJournal of Inorganic Biochemistryen_HK
dc.rightsJournal of Inorganic Biochemistry. Copyright © Elsevier Inc.en_HK
dc.subjectDNA cleavageen_HK
dc.subjectGuanidiniumen_HK
dc.subjectInhibitionen_HK
dc.subjectPhosphate diesteren_HK
dc.titlePhosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0162-0134&volume=103&spage=851&epage=858&date=2009&atitle=Phosphodiester+hydrolysis+and+specific+DNA+binding+and+cleavage+promoted+by+guanidinium-functionalized+zinc+en_HK
dc.identifier.emailSun, H:hsun@hkucc.hku.hken_HK
dc.identifier.authoritySun, H=rp00777en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jinorgbio.2009.02.010en_HK
dc.identifier.pmid19344953-
dc.identifier.scopuseid_2-s2.0-64449086446en_HK
dc.identifier.hkuros158586en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64449086446&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue5en_HK
dc.identifier.spage851en_HK
dc.identifier.epage858en_HK
dc.identifier.isiWOS:000265758200025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHe, J=35310626100en_HK
dc.identifier.scopusauthoridSun, J=7410367271en_HK
dc.identifier.scopusauthoridMao, ZW=23989068600en_HK
dc.identifier.scopusauthoridJi, LN=20734334600en_HK
dc.identifier.scopusauthoridSun, H=7404827446en_HK
dc.identifier.citeulike4572265-

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