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Article: Cyclometalated platinum(II) complexes as highly sensitive luminescent switch-on probes for practical application in protein staining and cell imaging

TitleCyclometalated platinum(II) complexes as highly sensitive luminescent switch-on probes for practical application in protein staining and cell imaging
Authors
Wu, PWong, ELMMa, DLTong, GSMNg, KMChe, CM
KeywordsCell imaging
Gels
Luminescence
Noncovalent interactions
Platinum
Protein staining
Issue Date2009
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/chemistry
Citation
Chemistry - A European Journal, 2009, v. 15 n. 15, p. 3652-3656 How to Cite?
DOI: http://dx.doi.org/10.1002/chem.200802707
AbstractA method to prepare highly luminescent cyclometalated platinum(II) complexes as switch-on probes for application in protein staining and cell imaging, was reported. The finished products showed several properties like MLCT emission in the visible spectral region, solubility and stability in aqueous staining solutions at room temperature, and binding to proteins through non-covalent interaction. The UV-visible absorption and NMR spectroscopy used for the study revealed that platinum(II) complexes remained stable in aqueous solution for 72 hours at room temperature. The cyclometalated platinum(II) complexes acted as protein-binding probes that can be used for the staining of protein mixtures in 1D and 2D SDS-PAGE without interfering with peptide-mass fingerprinting analysis and for live cell imaging. The MLCT emission of platinum(II) complexes were sensitive to auxiliary ligands.
Persistent Identifierhttp://hdl.handle.net/10722/58340
ISSN
0947-6539
2023 Impact Factor: 3.9
2023 SCImago Journal Rankings: 1.058
ISI Accession Number ID
WOS:000265162900002
Funding AgencyGrant Number
University Grants CommitteeAoE/P-10/01
The University of Hong Kong (University Development Fund)
Funding Information:

This work is supported by the Area of Excellence Scheme established under the University Grants Committee (HKSAR, China (AoE/P-10/01) and The University of Hong Kong (University Development Fund).

References
References in Scopus
Grants
Institute of molecular technology for drug discovery and synthesis

 

DC FieldValueLanguage
dc.contributor.authorWu, Pen_HK
dc.contributor.authorWong, ELMen_HK
dc.contributor.authorMa, DLen_HK
dc.contributor.authorTong, GSMen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorChe, CMen_HK
dc.date.accessioned2010-05-31T03:28:36Z-
dc.date.available2010-05-31T03:28:36Z-
dc.date.issued2009en_HK
dc.identifier.citationChemistry - A European Journal, 2009, v. 15 n. 15, p. 3652-3656en_HK
dc.identifier.issn0947-6539en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58340-
dc.description.abstractA method to prepare highly luminescent cyclometalated platinum(II) complexes as switch-on probes for application in protein staining and cell imaging, was reported. The finished products showed several properties like MLCT emission in the visible spectral region, solubility and stability in aqueous staining solutions at room temperature, and binding to proteins through non-covalent interaction. The UV-visible absorption and NMR spectroscopy used for the study revealed that platinum(II) complexes remained stable in aqueous solution for 72 hours at room temperature. The cyclometalated platinum(II) complexes acted as protein-binding probes that can be used for the staining of protein mixtures in 1D and 2D SDS-PAGE without interfering with peptide-mass fingerprinting analysis and for live cell imaging. The MLCT emission of platinum(II) complexes were sensitive to auxiliary ligands.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/chemistryen_HK
dc.relation.ispartofChemistry - A European Journalen_HK
dc.subjectCell imagingen_HK
dc.subjectGelsen_HK
dc.subjectLuminescenceen_HK
dc.subjectNoncovalent interactionsen_HK
dc.subjectPlatinumen_HK
dc.subjectProtein stainingen_HK
dc.titleCyclometalated platinum(II) complexes as highly sensitive luminescent switch-on probes for practical application in protein staining and cell imagingen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, ELM:wongella@hku.hken_HK
dc.identifier.emailMa, DL:edmondma@hku.hken_HK
dc.identifier.emailTong, GSM:tongsm@hkucc.hku.hken_HK
dc.identifier.emailNg, KM:kwanmng@hku.hken_HK
dc.identifier.emailChe, CM:cmche@hku.hken_HK
dc.identifier.authorityWong, ELM=rp00807en_HK
dc.identifier.authorityMa, DL=rp00760en_HK
dc.identifier.authorityTong, GSM=rp00790en_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/chem.200802707en_HK
dc.identifier.pmid19204965-
dc.identifier.scopuseid_2-s2.0-63849115228en_HK
dc.identifier.hkuros155857en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63849115228&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue15en_HK
dc.identifier.spage3652en_HK
dc.identifier.epage3656en_HK
dc.identifier.isiWOS:000265162900002-
dc.publisher.placeGermanyen_HK
dc.relation.projectInstitute of molecular technology for drug discovery and synthesis-
dc.identifier.scopusauthoridWu, P=7403119736en_HK
dc.identifier.scopusauthoridWong, ELM=8944839700en_HK
dc.identifier.scopusauthoridMa, DL=7402075538en_HK
dc.identifier.scopusauthoridTong, GSM=7102328656en_HK
dc.identifier.scopusauthoridNg, KM=26026091100en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.issnl0947-6539-

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