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Article: Study on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)

TitleStudy on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)
Authors
KeywordsCell bioassay
Metallothionein (MT)
Mutation
Neuronal growth inhibitory factor (GIF)
Structure-function relationship
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbio
Citation
Journal Of Inorganic Biochemistry, 2008, v. 102 n. 11, p. 1965-1972 How to Cite?
AbstractHuman metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6CPCP 9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58315
ISSN
2015 Impact Factor: 3.205
2015 SCImago Journal Rankings: 0.983
ISI Accession Number ID
Funding AgencyGrant Number
ational Science Foundation of China (NSFC)
Funding Information:

This work was supported by the National Science Foundation of China (NSFC). We appreciate Professor J.H.R. Kagi from Department of Biochemistry of the University of Zurich for his encouragement and fruitful discussion.

References

 

DC FieldValueLanguage
dc.contributor.authorDing, ZCen_HK
dc.contributor.authorZheng, Qen_HK
dc.contributor.authorCai, Ben_HK
dc.contributor.authorNi, FYen_HK
dc.contributor.authorYu, WHen_HK
dc.contributor.authorTeng, XCen_HK
dc.contributor.authorGao, Yen_HK
dc.contributor.authorLiu, Fen_HK
dc.contributor.authorChen, Den_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorWu, HMen_HK
dc.contributor.authorSun, HZen_HK
dc.contributor.authorZhang, MJen_HK
dc.contributor.authorTan, XSen_HK
dc.contributor.authorHuang, ZXen_HK
dc.date.accessioned2010-05-31T03:28:09Z-
dc.date.available2010-05-31T03:28:09Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Inorganic Biochemistry, 2008, v. 102 n. 11, p. 1965-1972en_HK
dc.identifier.issn0162-0134en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58315-
dc.description.abstractHuman metallothionein-3 (hMT3), also named as human neuronal growth inhibitory factor (hGIF), can inhibit the outgrowth of embryonic cortical neurons in the presence of brain extracts. In order to systematically study the structure-property-reactivity-function relationship of hGIF, our laboratory designed a series of mutants and studied their structure, property, reactivity and functions by a series of chemical and biological tools including UV spectroscopy, CD spectroscopy, NMR, chemical reaction and primary neuronal culture assays. In summary, we concluded that the bioactivity of hGIF was regulated by multiple factors, including the 6CPCP 9 motif, an additional threonine insert at sequence position 5, domain-domain interactions, the structure and stability of the metal-thiolate cluster and the linker. Our studies provide more and more evidences which revealed that the bioactivity of hGIF is mainly related to the essential metal release and its characteristic conformation. © 2008 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jinorgbioen_HK
dc.relation.ispartofJournal of Inorganic Biochemistryen_HK
dc.rightsJournal of Inorganic Biochemistry . Copyright © Elsevier Inc.en_HK
dc.subjectCell bioassayen_HK
dc.subjectMetallothionein (MT)en_HK
dc.subjectMutationen_HK
dc.subjectNeuronal growth inhibitory factor (GIF)en_HK
dc.subjectStructure-function relationshipen_HK
dc.titleStudy on structure-property-reactivity-function relationship of human neuronal growth inhibitory factor (hGIF)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0162-0134&volume=102&spage=1965&epage=1972&date=2008&atitle=Study+on+Structure-Property-Reactivity-Function+Relationship+of+Human+Neuronal+Growth+Inhibitory+Factor+(hGIF)+en_HK
dc.identifier.emailSun, HZ:hsun@hkucc.hku.hken_HK
dc.identifier.authoritySun, HZ=rp00777en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jinorgbio.2008.07.007en_HK
dc.identifier.pmid18757100-
dc.identifier.scopuseid_2-s2.0-53249140316en_HK
dc.identifier.hkuros158582en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53249140316&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume102en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1965en_HK
dc.identifier.epage1972en_HK
dc.identifier.isiWOS:000260363400001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDing, ZC=14424225400en_HK
dc.identifier.scopusauthoridZheng, Q=36887872800en_HK
dc.identifier.scopusauthoridCai, B=36484162900en_HK
dc.identifier.scopusauthoridNi, FY=9841840800en_HK
dc.identifier.scopusauthoridYu, WH=55205334100en_HK
dc.identifier.scopusauthoridTeng, XC=24725403100en_HK
dc.identifier.scopusauthoridGao, Y=34975077300en_HK
dc.identifier.scopusauthoridLiu, F=36062739500en_HK
dc.identifier.scopusauthoridChen, D=7405455814en_HK
dc.identifier.scopusauthoridWang, Y=36078812500en_HK
dc.identifier.scopusauthoridWu, HM=13808047800en_HK
dc.identifier.scopusauthoridSun, HZ=7404827446en_HK
dc.identifier.scopusauthoridZhang, MJ=7601555100en_HK
dc.identifier.scopusauthoridTan, XS=7202120913en_HK
dc.identifier.scopusauthoridHuang, ZX=7406221847en_HK

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