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Article: MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis

TitleMT1-MMP-Mediated cleavage of decorin in corneal angiogenesis
Authors
KeywordsAngiogenesis
Corneal neovascularization
Decorin
Extracellular matrix
Membrane type 1-matrix metalloproteinase
Metalloproteinase
MMP-14
Issue Date2009
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
Journal Of Vascular Research, 2009, v. 46 n. 6, p. 541-550 How to Cite?
AbstractBackground/Aims: Decorin has been shown to have antiangiogenic properties. In this study, we evaluate the involvement of membrane type 1-matrix metalloproteinase (MT1-MMP), a proangiogenic enzyme, in decorin cleavage in the cornea. Methods: MT1-MMP expression was confirmed immunohistochemically in keratocytes and immortalized corneal fibroblast cell lines. Corneal micropockets of bFGF were used to assess the expression of decorin and MT1-MMP. Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and stimulatory effect of MT1-MMP on vascular endothelial cells in vitro. Results: We show that MT1-MMP expression is upregulated following bFGF pellet implantation in the cornea in vivo, and that MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. Furthermore, the addition of MT1-MMP reduces the inhibitory effects of decorin on aortic ring tube formation in vitro. Cleavage of decorin by MT1-MMP-deficient corneal cell lysates is diminished relative to that by wild-type corneal cell lysates, and an MT1-MMP knockin restores decorin processing in vitro. Conclusion: The proangiogenic role of MT1-MMP in the cornea may be mediated, in part, by facilitated cleavage of corneal decorin. © 2009 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/58308
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119
ISI Accession Number ID
Funding AgencyGrant Number
National Institutes of HealthEY10101
P30EY001792
EY14048
Funding Information:

This study was funded by grants from the National Institutes of Health EY10101 (D. T. A.), P30EY001792 (D. T. A.), and EY14048 (J.- H. C.).

References

 

DC FieldValueLanguage
dc.contributor.authorMimura, Ten_HK
dc.contributor.authorHan, KYen_HK
dc.contributor.authorOnguchi, Ten_HK
dc.contributor.authorChang, JHen_HK
dc.contributor.authorKim, TIen_HK
dc.contributor.authorKojima, Ten_HK
dc.contributor.authorZhou, Zen_HK
dc.contributor.authorAzar, DTen_HK
dc.date.accessioned2010-05-31T03:27:51Z-
dc.date.available2010-05-31T03:27:51Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Vascular Research, 2009, v. 46 n. 6, p. 541-550en_HK
dc.identifier.issn1018-1172en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58308-
dc.description.abstractBackground/Aims: Decorin has been shown to have antiangiogenic properties. In this study, we evaluate the involvement of membrane type 1-matrix metalloproteinase (MT1-MMP), a proangiogenic enzyme, in decorin cleavage in the cornea. Methods: MT1-MMP expression was confirmed immunohistochemically in keratocytes and immortalized corneal fibroblast cell lines. Corneal micropockets of bFGF were used to assess the expression of decorin and MT1-MMP. Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and stimulatory effect of MT1-MMP on vascular endothelial cells in vitro. Results: We show that MT1-MMP expression is upregulated following bFGF pellet implantation in the cornea in vivo, and that MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. Furthermore, the addition of MT1-MMP reduces the inhibitory effects of decorin on aortic ring tube formation in vitro. Cleavage of decorin by MT1-MMP-deficient corneal cell lysates is diminished relative to that by wild-type corneal cell lysates, and an MT1-MMP knockin restores decorin processing in vitro. Conclusion: The proangiogenic role of MT1-MMP in the cornea may be mediated, in part, by facilitated cleavage of corneal decorin. © 2009 S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVRen_HK
dc.relation.ispartofJournal of Vascular Researchen_HK
dc.rightsJournal of Vascular Research. Copyright © S Karger AG.en_HK
dc.subjectAngiogenesisen_HK
dc.subjectCorneal neovascularizationen_HK
dc.subjectDecorinen_HK
dc.subjectExtracellular matrixen_HK
dc.subjectMembrane type 1-matrix metalloproteinaseen_HK
dc.subjectMetalloproteinaseen_HK
dc.subjectMMP-14en_HK
dc.subject.meshCornea - enzymology-
dc.subject.meshCorneal Neovascularization - chemically induced - enzymology-
dc.subject.meshExtracellular Matrix Proteins - metabolism-
dc.subject.meshMatrix Metalloproteinase 14 - antagonists and inhibitors - deficiency - genetics - metabolism-
dc.subject.meshProteoglycans - metabolism-
dc.titleMT1-MMP-Mediated cleavage of decorin in corneal angiogenesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=46&issue=6&spage=541&epage=550&date=2009&atitle=MT1-MMP-mediated+cleavage+of+decorin+in+corneal+angiogenesisen_HK
dc.identifier.emailZhou, Z:zhongjun@hkucc.hku.hken_HK
dc.identifier.authorityZhou, Z=rp00503en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000226222en_HK
dc.identifier.pmid19571574-
dc.identifier.scopuseid_2-s2.0-67649424445en_HK
dc.identifier.hkuros167558en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67649424445&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue6en_HK
dc.identifier.spage541en_HK
dc.identifier.epage550en_HK
dc.identifier.isiWOS:000270780200003-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridMimura, T=35242662100en_HK
dc.identifier.scopusauthoridHan, KY=7402960197en_HK
dc.identifier.scopusauthoridOnguchi, T=8267619500en_HK
dc.identifier.scopusauthoridChang, JH=22333289500en_HK
dc.identifier.scopusauthoridKim, TI=35214969200en_HK
dc.identifier.scopusauthoridKojima, T=7403447572en_HK
dc.identifier.scopusauthoridZhou, Z=8631856300en_HK
dc.identifier.scopusauthoridAzar, DT=26643368400en_HK

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