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- PMID: 19571574
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Article: MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis
Title | MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis | ||||
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Authors | |||||
Keywords | Angiogenesis Corneal neovascularization Decorin Extracellular matrix Membrane type 1-matrix metalloproteinase Metalloproteinase MMP-14 | ||||
Issue Date | 2009 | ||||
Publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/JVR | ||||
Citation | Journal Of Vascular Research, 2009, v. 46 n. 6, p. 541-550 How to Cite? | ||||
Abstract | Background/Aims: Decorin has been shown to have antiangiogenic properties. In this study, we evaluate the involvement of membrane type 1-matrix metalloproteinase (MT1-MMP), a proangiogenic enzyme, in decorin cleavage in the cornea. Methods: MT1-MMP expression was confirmed immunohistochemically in keratocytes and immortalized corneal fibroblast cell lines. Corneal micropockets of bFGF were used to assess the expression of decorin and MT1-MMP. Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and stimulatory effect of MT1-MMP on vascular endothelial cells in vitro. Results: We show that MT1-MMP expression is upregulated following bFGF pellet implantation in the cornea in vivo, and that MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. Furthermore, the addition of MT1-MMP reduces the inhibitory effects of decorin on aortic ring tube formation in vitro. Cleavage of decorin by MT1-MMP-deficient corneal cell lysates is diminished relative to that by wild-type corneal cell lysates, and an MT1-MMP knockin restores decorin processing in vitro. Conclusion: The proangiogenic role of MT1-MMP in the cornea may be mediated, in part, by facilitated cleavage of corneal decorin. © 2009 S. Karger AG, Basel. | ||||
Persistent Identifier | http://hdl.handle.net/10722/58308 | ||||
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.486 | ||||
ISI Accession Number ID |
Funding Information: This study was funded by grants from the National Institutes of Health EY10101 (D. T. A.), P30EY001792 (D. T. A.), and EY14048 (J.- H. C.). | ||||
References |
DC Field | Value | Language |
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dc.contributor.author | Mimura, T | en_HK |
dc.contributor.author | Han, KY | en_HK |
dc.contributor.author | Onguchi, T | en_HK |
dc.contributor.author | Chang, JH | en_HK |
dc.contributor.author | Kim, TI | en_HK |
dc.contributor.author | Kojima, T | en_HK |
dc.contributor.author | Zhou, Z | en_HK |
dc.contributor.author | Azar, DT | en_HK |
dc.date.accessioned | 2010-05-31T03:27:51Z | - |
dc.date.available | 2010-05-31T03:27:51Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Vascular Research, 2009, v. 46 n. 6, p. 541-550 | en_HK |
dc.identifier.issn | 1018-1172 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/58308 | - |
dc.description.abstract | Background/Aims: Decorin has been shown to have antiangiogenic properties. In this study, we evaluate the involvement of membrane type 1-matrix metalloproteinase (MT1-MMP), a proangiogenic enzyme, in decorin cleavage in the cornea. Methods: MT1-MMP expression was confirmed immunohistochemically in keratocytes and immortalized corneal fibroblast cell lines. Corneal micropockets of bFGF were used to assess the expression of decorin and MT1-MMP. Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and stimulatory effect of MT1-MMP on vascular endothelial cells in vitro. Results: We show that MT1-MMP expression is upregulated following bFGF pellet implantation in the cornea in vivo, and that MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. Furthermore, the addition of MT1-MMP reduces the inhibitory effects of decorin on aortic ring tube formation in vitro. Cleavage of decorin by MT1-MMP-deficient corneal cell lysates is diminished relative to that by wild-type corneal cell lysates, and an MT1-MMP knockin restores decorin processing in vitro. Conclusion: The proangiogenic role of MT1-MMP in the cornea may be mediated, in part, by facilitated cleavage of corneal decorin. © 2009 S. Karger AG, Basel. | en_HK |
dc.language | eng | en_HK |
dc.publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/JVR | en_HK |
dc.relation.ispartof | Journal of Vascular Research | en_HK |
dc.rights | Journal of Vascular Research. Copyright © S Karger AG. | en_HK |
dc.subject | Angiogenesis | en_HK |
dc.subject | Corneal neovascularization | en_HK |
dc.subject | Decorin | en_HK |
dc.subject | Extracellular matrix | en_HK |
dc.subject | Membrane type 1-matrix metalloproteinase | en_HK |
dc.subject | Metalloproteinase | en_HK |
dc.subject | MMP-14 | en_HK |
dc.subject.mesh | Cornea - enzymology | - |
dc.subject.mesh | Corneal Neovascularization - chemically induced - enzymology | - |
dc.subject.mesh | Extracellular Matrix Proteins - metabolism | - |
dc.subject.mesh | Matrix Metalloproteinase 14 - antagonists and inhibitors - deficiency - genetics - metabolism | - |
dc.subject.mesh | Proteoglycans - metabolism | - |
dc.title | MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=46&issue=6&spage=541&epage=550&date=2009&atitle=MT1-MMP-mediated+cleavage+of+decorin+in+corneal+angiogenesis | en_HK |
dc.identifier.email | Zhou, Z:zhongjun@hkucc.hku.hk | en_HK |
dc.identifier.authority | Zhou, Z=rp00503 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1159/000226222 | en_HK |
dc.identifier.pmid | 19571574 | - |
dc.identifier.scopus | eid_2-s2.0-67649424445 | en_HK |
dc.identifier.hkuros | 167558 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67649424445&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 46 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 541 | en_HK |
dc.identifier.epage | 550 | en_HK |
dc.identifier.isi | WOS:000270780200003 | - |
dc.publisher.place | Switzerland | en_HK |
dc.identifier.scopusauthorid | Mimura, T=35242662100 | en_HK |
dc.identifier.scopusauthorid | Han, KY=7402960197 | en_HK |
dc.identifier.scopusauthorid | Onguchi, T=8267619500 | en_HK |
dc.identifier.scopusauthorid | Chang, JH=22333289500 | en_HK |
dc.identifier.scopusauthorid | Kim, TI=35214969200 | en_HK |
dc.identifier.scopusauthorid | Kojima, T=7403447572 | en_HK |
dc.identifier.scopusauthorid | Zhou, Z=8631856300 | en_HK |
dc.identifier.scopusauthorid | Azar, DT=26643368400 | en_HK |
dc.identifier.issnl | 1018-1172 | - |