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Article: The complete genome and proteome of laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats

TitleThe complete genome and proteome of laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitats
Authors
Issue Date2009
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosgenetics.org/
Citation
Plos Genetics, 2009, v. 5 n. 3 How to Cite?
AbstractLaribacter hongkongensis is a newly discovered Gram-negative bacillus of the Neisseriaceae family associated with freshwater fish-borne gastroenteritis and traveler's diarrhea. The complete genome sequence of L. hongkongensis HLHK9, recovered from an immunocompetent patient with severe gastroenteritis, consists of a 3,169-kb chromosome with G+C content of 62.35%. Genome analysis reveals different mechanisms potentially important for its adaptation to diverse habitats of human and freshwater fish intestines and freshwater environments. The gene contents support its phenotypic properties and suggest that amino acids and fatty acids can be used as carbon sources. The extensive variety of transporters, including multidrug efflux and heavy metal transporters as well as genes involved in chemotaxis, may enable L. hongkongensis to survive in different environmental niches. Genes encoding urease, bile salts efflux pump, adhesin, catalase, superoxide dismutase, and other putative virulence factors-such as hemolysins, RTX toxins, patatin-like proteins, phospholipase A1, and collagenases-are present. Proteomes of L. hongkongensis HLHK9 cultured at 37°C (human body temperature) and 20°C (freshwater habitat temperature) showed differential gene expression, including two homologous copies of argB, argB-20, and argB-37, which encode two isoenzymes of N-acetyl-L-glutamate kinase (NAGK)-NAGK-20 and NAGK-37-in the arginine biosynthesis pathway. NAGK-20 showed higher expression at 20°C, whereas NAGK-37 showed higher expression at 37°C. NAGK-20 also had a lower optimal temperature for enzymatic activities and was inhibited by arginine probably as negative-feedback control. Similar duplicated copies of argB are also observed in bacteria from hot springs such as Thermus thermophilus, Deinococcus geothermalis, Deinococcus radiodurans, and Roseiflexus castenholzii, suggesting that similar mechanisms for temperature adaptation may be employed by other bacteria. Genome and proteome analysis of L. hongkongensis revealed novel mechanisms for adaptations to survival at different temperatures and habitats. Copyright: © 2009 Woo et al.
Persistent Identifierhttp://hdl.handle.net/10722/58301
ISSN
2014 Impact Factor: 7.528
2015 SCImago Journal Rankings: 6.308
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
HKSAR RFCID of the Health, Welfare and Food Bureau
RGC
University Development Fund
Outstanding Young Researcher Award
University of Hong Kong
British Biotechnology and Biological Research Council
Funding Information:

This work is partly supported by the HKSAR RFCID of the Health, Welfare and Food Bureau; the RGC Grant; University Development Fund and Outstanding Young Researcher Award, The University of Hong Kong. Work in the Pallen group was supported by the British Biotechnology and Biological Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorCurreem, SOTen_HK
dc.contributor.authorTsang, AKLen_HK
dc.contributor.authorFan, RYYen_HK
dc.contributor.authorWong, GKMen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorLoman, NJen_HK
dc.contributor.authorSnyder, LASen_HK
dc.contributor.authorCai, JJen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorMak, Wen_HK
dc.contributor.authorPallen, MJen_HK
dc.contributor.authorLok, Sen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-05-31T03:27:44Z-
dc.date.available2010-05-31T03:27:44Z-
dc.date.issued2009en_HK
dc.identifier.citationPlos Genetics, 2009, v. 5 n. 3en_HK
dc.identifier.issn1553-7390en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58301-
dc.description.abstractLaribacter hongkongensis is a newly discovered Gram-negative bacillus of the Neisseriaceae family associated with freshwater fish-borne gastroenteritis and traveler's diarrhea. The complete genome sequence of L. hongkongensis HLHK9, recovered from an immunocompetent patient with severe gastroenteritis, consists of a 3,169-kb chromosome with G+C content of 62.35%. Genome analysis reveals different mechanisms potentially important for its adaptation to diverse habitats of human and freshwater fish intestines and freshwater environments. The gene contents support its phenotypic properties and suggest that amino acids and fatty acids can be used as carbon sources. The extensive variety of transporters, including multidrug efflux and heavy metal transporters as well as genes involved in chemotaxis, may enable L. hongkongensis to survive in different environmental niches. Genes encoding urease, bile salts efflux pump, adhesin, catalase, superoxide dismutase, and other putative virulence factors-such as hemolysins, RTX toxins, patatin-like proteins, phospholipase A1, and collagenases-are present. Proteomes of L. hongkongensis HLHK9 cultured at 37°C (human body temperature) and 20°C (freshwater habitat temperature) showed differential gene expression, including two homologous copies of argB, argB-20, and argB-37, which encode two isoenzymes of N-acetyl-L-glutamate kinase (NAGK)-NAGK-20 and NAGK-37-in the arginine biosynthesis pathway. NAGK-20 showed higher expression at 20°C, whereas NAGK-37 showed higher expression at 37°C. NAGK-20 also had a lower optimal temperature for enzymatic activities and was inhibited by arginine probably as negative-feedback control. Similar duplicated copies of argB are also observed in bacteria from hot springs such as Thermus thermophilus, Deinococcus geothermalis, Deinococcus radiodurans, and Roseiflexus castenholzii, suggesting that similar mechanisms for temperature adaptation may be employed by other bacteria. Genome and proteome analysis of L. hongkongensis revealed novel mechanisms for adaptations to survival at different temperatures and habitats. Copyright: © 2009 Woo et al.en_HK
dc.languageengen_HK
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosgenetics.org/en_HK
dc.relation.ispartofPLoS Geneticsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAdaptation, Physiological - genetics-
dc.subject.meshGenome, Bacterial-
dc.subject.meshNeisseriaceae - genetics-
dc.subject.meshProteome-
dc.subject.meshTemperature-
dc.titleThe complete genome and proteome of laribacter hongkongensis reveal potential mechanisms for adaptations to different temperatures and habitatsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1553-7390&volume=5&issue=3, article no. e1000416&spage=&epage=&date=2009&atitle=The+complete+genome+and+proteome+of+Laribacter+hongkongensis+reveal+potential+mechanisms+for+adaptations+to+different+temperatures+and+habitats-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hken_HK
dc.identifier.emailTse, H: htse@hkucc.hku.hken_HK
dc.identifier.emailTeng, JLL: llteng@hku.hken_HK
dc.identifier.emailHuang, JD: jdhuang@hku.hken_HK
dc.identifier.emailLok, S: silok@genome.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityLok, S=rp00271en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pgen.1000416en_HK
dc.identifier.pmid19283063-
dc.identifier.pmcidPMC2652115-
dc.identifier.scopuseid_2-s2.0-63549135873en_HK
dc.identifier.hkuros192187en_HK
dc.identifier.hkuros160599en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63549135873&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.eissn1553-7404-
dc.identifier.isiWOS:000266320100022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridCurreem, SOT=16416762100en_HK
dc.identifier.scopusauthoridTsang, AKL=7006979247en_HK
dc.identifier.scopusauthoridFan, RYY=15519269300en_HK
dc.identifier.scopusauthoridWong, GKM=9333006300en_HK
dc.identifier.scopusauthoridHuang, Y=35597414700en_HK
dc.identifier.scopusauthoridLoman, NJ=6701414703en_HK
dc.identifier.scopusauthoridSnyder, LAS=7202948191en_HK
dc.identifier.scopusauthoridCai, JJ=7403153560en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridMak, W=22934897600en_HK
dc.identifier.scopusauthoridPallen, MJ=7005883114en_HK
dc.identifier.scopusauthoridLok, S=21035019900en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike4179719-

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