File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Examination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus

TitleExamination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virus
Authors
KeywordsHuman coronavirus HKU1
Neutralization antibody
Seroepidemiology
Spike
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2009, v. 45 n. 1, p. 54-60 How to Cite?
AbstractBackground: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections. Objectives: To investigate the seroepidemiology of HKU1 infections in our local population. Study design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA. Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, from 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old. Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58290
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council General Research Fund781008M
St. Paul's Hospital Professional Development Fund
HKU Special Research Achievement Award
HKU Internal Award
Funding Information:

This work was partly supported by a Research Grants Council General Research Fund grant (781008M), St. Paul's Hospital Professional Development Fund, HKU Special Research Achievement Award, and HKU Internal Award for CAE Membership.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, CMen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-05-31T03:27:32Z-
dc.date.available2010-05-31T03:27:32Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Clinical Virology, 2009, v. 45 n. 1, p. 54-60en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58290-
dc.description.abstractBackground: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections. Objectives: To investigate the seroepidemiology of HKU1 infections in our local population. Study design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA. Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, from 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old. Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.subjectHuman coronavirus HKU1en_HK
dc.subjectNeutralization antibodyen_HK
dc.subjectSeroepidemiologyen_HK
dc.subjectSpikeen_HK
dc.subject.meshAntibodies, Viral - blood-
dc.subject.meshCoronavirus - immunology-
dc.subject.meshCoronavirus Infections - epidemiology - immunology-
dc.subject.meshEnzyme-Linked Immunosorbent Assay-
dc.subject.meshMembrane Glycoproteins - immunology-
dc.titleExamination of seroprevalence of coronavirus HKU1 infection with S protein-based ELISA and neutralization assay against viral spike pseudotyped virusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=45&spage=54&epage=60&date=2009&atitle=Examination+of+seroprevalence+of+coronavirus+HKU1+infection+with+S+protein-based+ELISA+and+neutralization+assay+against+viral+spike+pseudotyped+virusen_HK
dc.identifier.emailTse, H:herman@graduate.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcv.2009.02.011en_HK
dc.identifier.pmid19342289-
dc.identifier.scopuseid_2-s2.0-67349188881en_HK
dc.identifier.hkuros160597en_HK
dc.identifier.hkuros156488-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349188881&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue1en_HK
dc.identifier.spage54en_HK
dc.identifier.epage60en_HK
dc.identifier.isiWOS:000266682900011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridChen, L=7409435006en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.citeulike4671437-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats