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Article: Melatonin as a negative mitogenic hormonal regulator of human prostate epithelial cell growth: Potential mechanisms and clinical significance

TitleMelatonin as a negative mitogenic hormonal regulator of human prostate epithelial cell growth: Potential mechanisms and clinical significance
Authors
KeywordsMelatonin
Mitogenic regulator
MT 1 receptor
p27 Kip1
Prostate
Issue Date2008
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPI
Citation
Journal Of Pineal Research, 2008, v. 45 n. 4, p. 403-412 How to Cite?
AbstractCircannual variation in the human serum levels of prostate-specific antigen, a growth marker of the prostate gland, has been reported recently. The present study was conducted to investigate the role of the photoperiodic hormone melatonin (MLT) and its membrane receptors in the modulation of human prostate growth. Expression of MT 1 and MT 2 receptors was detected in benign human prostatic epithelial tissues and RWPE-1 cells. MLT and 2-iodomelatonin inhibited RWPE-1 cell proliferation and up-regulated p27 Kip1 gene and protein expression in the cells. The effects of MLT were blocked by the nonselective MT 1/MT 2 receptor antagonist luzindole, but were not affected by the selective MT 2 receptor antagonist 4-phenyl-2-propionamidotetraline. Of note, the antiproliferative action of MLT on benign prostate epithelial RWPE-1 cells was effected via increased p27 Kip1 gene transcription through MT 1 receptor-mediated activation of protein kinase A (PKA) and protein kinase C (PKC) in parallel, a signaling process which has previously been demonstrated in 22Rv1 prostate cancer cells. Taken together, the demonstration of the MT 1/PKA+PKC/p27 Kip1 antiproliferative pathway in benign and malignant prostate epithelial cell lines indicated the potential importance of this MLT receptor-mediated signaling mechanism in growth regulation of the human prostate gland in health and disease. Collectively, our data support the hypothesis that MLT may function as a negative mitogenic hormonal regulator of human prostate epithelial cell growth. © 2008 The Authors.
Persistent Identifierhttp://hdl.handle.net/10722/58285
ISSN
2015 Impact Factor: 9.314
2015 SCImago Journal Rankings: 2.655
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200707176075
Funding Information:

This work was supported by the small project funding scheme (project code: 200707176075) of The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorTam, CWen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorPang, Ben_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-05-31T03:27:26Z-
dc.date.available2010-05-31T03:27:26Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Pineal Research, 2008, v. 45 n. 4, p. 403-412en_HK
dc.identifier.issn0742-3098en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58285-
dc.description.abstractCircannual variation in the human serum levels of prostate-specific antigen, a growth marker of the prostate gland, has been reported recently. The present study was conducted to investigate the role of the photoperiodic hormone melatonin (MLT) and its membrane receptors in the modulation of human prostate growth. Expression of MT 1 and MT 2 receptors was detected in benign human prostatic epithelial tissues and RWPE-1 cells. MLT and 2-iodomelatonin inhibited RWPE-1 cell proliferation and up-regulated p27 Kip1 gene and protein expression in the cells. The effects of MLT were blocked by the nonselective MT 1/MT 2 receptor antagonist luzindole, but were not affected by the selective MT 2 receptor antagonist 4-phenyl-2-propionamidotetraline. Of note, the antiproliferative action of MLT on benign prostate epithelial RWPE-1 cells was effected via increased p27 Kip1 gene transcription through MT 1 receptor-mediated activation of protein kinase A (PKA) and protein kinase C (PKC) in parallel, a signaling process which has previously been demonstrated in 22Rv1 prostate cancer cells. Taken together, the demonstration of the MT 1/PKA+PKC/p27 Kip1 antiproliferative pathway in benign and malignant prostate epithelial cell lines indicated the potential importance of this MLT receptor-mediated signaling mechanism in growth regulation of the human prostate gland in health and disease. Collectively, our data support the hypothesis that MLT may function as a negative mitogenic hormonal regulator of human prostate epithelial cell growth. © 2008 The Authors.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPIen_HK
dc.relation.ispartofJournal of Pineal Researchen_HK
dc.subjectMelatoninen_HK
dc.subjectMitogenic regulatoren_HK
dc.subjectMT 1 receptoren_HK
dc.subjectp27 Kip1en_HK
dc.subjectProstateen_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCyclin-Dependent Kinase Inhibitor p27 - genetics - metabolismen_HK
dc.subject.meshEpithelial Cells - cytology - physiologyen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshGrowth Substances - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoblottingen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMelatonin - analogs & derivatives - pharmacology - physiologyen_HK
dc.subject.meshProstate - cytology - metabolism - pathologyen_HK
dc.subject.meshProstatic Neoplasms - metabolismen_HK
dc.subject.meshProtein Kinases - analysisen_HK
dc.subject.meshRNA, Small Interferingen_HK
dc.subject.meshReceptors, Androgen - metabolismen_HK
dc.subject.meshReceptors, Melatonin - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSignal Transduction - drug effects - physiologyen_HK
dc.subject.meshTetrahydronaphthalenes - pharmacologyen_HK
dc.subject.meshTryptamines - pharmacologyen_HK
dc.titleMelatonin as a negative mitogenic hormonal regulator of human prostate epithelial cell growth: Potential mechanisms and clinical significanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0742-3098&volume=45&spage=403&epage=412&date=2008&atitle=Melatonin+as+a+negative+mitogenic+hormonal+regulator+of+human+prostate+epithelial+cell+growth:+potential+mechanisms+and+clinical+significanceen_HK
dc.identifier.emailChan, KW: hrmtckw@hku.hken_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailYao, KM: kmyao@hku.hken_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-079X.2008.00608.xen_HK
dc.identifier.pmid18637986-
dc.identifier.scopuseid_2-s2.0-53749100168en_HK
dc.identifier.hkuros148670en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53749100168&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue4en_HK
dc.identifier.spage403en_HK
dc.identifier.epage412en_HK
dc.identifier.eissn1600-079X-
dc.identifier.isiWOS:000259951700007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridTam, CW=7201442977en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK
dc.identifier.scopusauthoridLiu, VWS=7006405113en_HK
dc.identifier.scopusauthoridPang, B=37038253900en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridShiu, SYW=7005550655en_HK
dc.identifier.citeulike3401835-

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