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Article: Spike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detection

TitleSpike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detection
Authors
KeywordsCoronavirus HKU1
Furin inhibitor
Glycoprotein
Life cycle
Maturation
Native conformation
Nglycosylation
Seroepidemiology
Spike
Surface expression
Virus maturation
Issue Date2008
PublisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/
Citation
Experimental Biology And Medicine, 2008, v. 233 n. 12, p. 1527-1536 How to Cite?
AbstractWe recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Copyright © 2008 by the Society for Experimental Biology and Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/58270
ISSN
2015 Impact Factor: 2.542
2015 SCImago Journal Rankings: 0.991
ISI Accession Number ID
Funding AgencyGrant Number
UGC Research Grants Council781008M
HKU Special Research Achievement Award
St. Paul's Hospital professional Development Fund
Funding Information:

This project was supported by General Research Fund from the UGC Research Grants Council (ref: 781008M), HKU Special Research Achievement Award and St. Paul's Hospital professional Development Fund.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, CMen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorChen, HLen_HK
dc.contributor.authorLi, Fen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-05-31T03:27:10Z-
dc.date.available2010-05-31T03:27:10Z-
dc.date.issued2008en_HK
dc.identifier.citationExperimental Biology And Medicine, 2008, v. 233 n. 12, p. 1527-1536en_HK
dc.identifier.issn1535-3702en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58270-
dc.description.abstractWe recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Copyright © 2008 by the Society for Experimental Biology and Medicine.en_HK
dc.languageengen_HK
dc.publisherSociety for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/en_HK
dc.relation.ispartofExperimental Biology and Medicineen_HK
dc.subjectCoronavirus HKU1en_HK
dc.subjectFurin inhibitoren_HK
dc.subjectGlycoproteinen_HK
dc.subjectLife cycleen_HK
dc.subjectMaturationen_HK
dc.subjectNative conformationen_HK
dc.subjectNglycosylationen_HK
dc.subjectSeroepidemiologyen_HK
dc.subjectSpikeen_HK
dc.subjectSurface expressionen_HK
dc.subjectVirus maturationen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies, Viral - genetics - immunology - metabolismen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCoronavirus - classification - genetics - immunology - metabolismen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshFluorescein-5-isothiocyanate - metabolismen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshFluorescent Dyes - metabolismen_HK
dc.subject.meshGlycosylationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunizationen_HK
dc.subject.meshKidney - cytologyen_HK
dc.subject.meshMembrane Glycoproteins - genetics - immunology - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshPhysiological Processes - geneticsen_HK
dc.subject.meshRecombinant Proteins - immunology - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.subject.meshViral Envelope Proteins - genetics - immunology - metabolismen_HK
dc.titleSpike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detectionen_HK
dc.typeArticleen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailTse, H:herman@graduate.hku.hken_HK
dc.identifier.emailChen, HL:hlchen@hkucc.hku.hken_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityChen, HL=rp00383en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3181/0806-RM-197en_HK
dc.identifier.pmid18849544-
dc.identifier.scopuseid_2-s2.0-58749109053en_HK
dc.identifier.hkuros156478en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58749109053&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume233en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1527en_HK
dc.identifier.epage1536en_HK
dc.identifier.isiWOS:000261561500010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridChen, HL=26643315400en_HK
dc.identifier.scopusauthoridLi, F=26643460100en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridChen, L=7409435006en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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