File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3181/0806-RM-197
- Scopus: eid_2-s2.0-58749109053
- PMID: 18849544
- WOS: WOS:000261561500010
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Spike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detection
Title | Spike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detection | ||||||||
---|---|---|---|---|---|---|---|---|---|
Authors | |||||||||
Keywords | Coronavirus HKU1 Furin inhibitor Glycoprotein Life cycle Maturation Native conformation Nglycosylation Seroepidemiology Spike Surface expression Virus maturation | ||||||||
Issue Date | 2008 | ||||||||
Publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ | ||||||||
Citation | Experimental Biology And Medicine, 2008, v. 233 n. 12, p. 1527-1536 How to Cite? | ||||||||
Abstract | We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Copyright © 2008 by the Society for Experimental Biology and Medicine. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/58270 | ||||||||
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.850 | ||||||||
ISI Accession Number ID |
Funding Information: This project was supported by General Research Fund from the UGC Research Grants Council (ref: 781008M), HKU Special Research Achievement Award and St. Paul's Hospital professional Development Fund. | ||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, CM | en_HK |
dc.contributor.author | Woo, PCY | en_HK |
dc.contributor.author | Lau, SKP | en_HK |
dc.contributor.author | Tse, H | en_HK |
dc.contributor.author | Chen, HL | en_HK |
dc.contributor.author | Li, F | en_HK |
dc.contributor.author | Zheng, BJ | en_HK |
dc.contributor.author | Chen, L | en_HK |
dc.contributor.author | Huang, JD | en_HK |
dc.contributor.author | Yuen, KY | en_HK |
dc.date.accessioned | 2010-05-31T03:27:10Z | - |
dc.date.available | 2010-05-31T03:27:10Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Experimental Biology And Medicine, 2008, v. 233 n. 12, p. 1527-1536 | en_HK |
dc.identifier.issn | 1535-3702 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/58270 | - |
dc.description.abstract | We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle. Copyright © 2008 by the Society for Experimental Biology and Medicine. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Society for Experimental Biology and Medicine. The Journal's web site is located at http://www.ebmonline.org/ | en_HK |
dc.relation.ispartof | Experimental Biology and Medicine | en_HK |
dc.subject | Coronavirus HKU1 | en_HK |
dc.subject | Furin inhibitor | en_HK |
dc.subject | Glycoprotein | en_HK |
dc.subject | Life cycle | en_HK |
dc.subject | Maturation | en_HK |
dc.subject | Native conformation | en_HK |
dc.subject | Nglycosylation | en_HK |
dc.subject | Seroepidemiology | en_HK |
dc.subject | Spike | en_HK |
dc.subject | Surface expression | en_HK |
dc.subject | Virus maturation | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Antibodies, Viral - genetics - immunology - metabolism | en_HK |
dc.subject.mesh | Cell Line | en_HK |
dc.subject.mesh | Coronavirus - classification - genetics - immunology - metabolism | en_HK |
dc.subject.mesh | Cricetinae | en_HK |
dc.subject.mesh | Fluorescein-5-isothiocyanate - metabolism | en_HK |
dc.subject.mesh | Fluorescent Antibody Technique, Indirect | en_HK |
dc.subject.mesh | Fluorescent Dyes - metabolism | en_HK |
dc.subject.mesh | Glycosylation | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Immunization | en_HK |
dc.subject.mesh | Kidney - cytology | en_HK |
dc.subject.mesh | Membrane Glycoproteins - genetics - immunology - metabolism | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Mice, Inbred BALB C | en_HK |
dc.subject.mesh | Physiological Processes - genetics | en_HK |
dc.subject.mesh | Recombinant Proteins - immunology - metabolism | en_HK |
dc.subject.mesh | Transfection | en_HK |
dc.subject.mesh | Viral Envelope Proteins - genetics - immunology - metabolism | en_HK |
dc.title | Spike protein, S, of human coronavirus HKU1: Role in viral life cycle and application in antibody detection | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Woo, PCY:pcywoo@hkucc.hku.hk | en_HK |
dc.identifier.email | Lau, SKP:skplau@hkucc.hku.hk | en_HK |
dc.identifier.email | Tse, H:herman@graduate.hku.hk | en_HK |
dc.identifier.email | Chen, HL:hlchen@hkucc.hku.hk | en_HK |
dc.identifier.email | Zheng, BJ:bzheng@hkucc.hku.hk | en_HK |
dc.identifier.email | Huang, JD:jdhuang@hkucc.hku.hk | en_HK |
dc.identifier.email | Yuen, KY:kyyuen@hkucc.hku.hk | en_HK |
dc.identifier.authority | Woo, PCY=rp00430 | en_HK |
dc.identifier.authority | Lau, SKP=rp00486 | en_HK |
dc.identifier.authority | Tse, H=rp00519 | en_HK |
dc.identifier.authority | Chen, HL=rp00383 | en_HK |
dc.identifier.authority | Zheng, BJ=rp00353 | en_HK |
dc.identifier.authority | Huang, JD=rp00451 | en_HK |
dc.identifier.authority | Yuen, KY=rp00366 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3181/0806-RM-197 | en_HK |
dc.identifier.pmid | 18849544 | - |
dc.identifier.scopus | eid_2-s2.0-58749109053 | en_HK |
dc.identifier.hkuros | 156478 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-58749109053&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 233 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 1527 | en_HK |
dc.identifier.epage | 1536 | en_HK |
dc.identifier.isi | WOS:000261561500010 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Chan, CM=7404814453 | en_HK |
dc.identifier.scopusauthorid | Woo, PCY=7201801340 | en_HK |
dc.identifier.scopusauthorid | Lau, SKP=7401596211 | en_HK |
dc.identifier.scopusauthorid | Tse, H=7006070596 | en_HK |
dc.identifier.scopusauthorid | Chen, HL=26643315400 | en_HK |
dc.identifier.scopusauthorid | Li, F=26643460100 | en_HK |
dc.identifier.scopusauthorid | Zheng, BJ=7201780588 | en_HK |
dc.identifier.scopusauthorid | Chen, L=7409435006 | en_HK |
dc.identifier.scopusauthorid | Huang, JD=8108660600 | en_HK |
dc.identifier.scopusauthorid | Yuen, KY=36078079100 | en_HK |
dc.identifier.issnl | 1535-3699 | - |