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Article: DNA fingerprinting tags novel altered chromosomal regions and identifies the involvement of SOX5 in the progression of prostate cancer

TitleDNA fingerprinting tags novel altered chromosomal regions and identifies the involvement of SOX5 in the progression of prostate cancer
Authors
KeywordsChromosome 12p12.1
LCM
Prostate cancer
SMAL-PCR DNA fingerprinting
SOX5
Issue Date2009
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2009, v. 124 n. 10, p. 2323-2332 How to Cite?
AbstractIdentification of genomic alterations associated with the progression of prostate cancer may facilitate the better understanding of the development of this highly variable disease. Matched normal, premalignant high-grade prostatic intraepithelial neoplasia and invasive prostate carcinoma cells were procured by laser capture microdissection (LCM) from human radical prostatectomy specimens. From these cells, comparative DNA fingerprints were generated by a modified PCR-based technique called scanning of microdissected archival lesion (SMAL)-PCR. Recurrent polymorphic fingerprint fragments were used in tagging altered chromosomal regions. Altered regions were found at cytobands 1p31.3, 1q44, 2p23.1, 3p26.3, 3q22.3, 4q22.3, 4q35.2, 5q23.2, 8q22.3, 8q24.13, 9q21.3, 9q22.32, 10q11.21, 11p13, 12p12.1, 13q12.1, 16q12.2 and 18q21.31. Candidate genes in the surrounding area that may possibly harbor mutations that change normal prostatic cells to progress into their tumor stages were proposed. Of these fragments, a 420 bp alteration, absent in all 26 normal samples screened, was observed in 2 tumors. This fragment was cloned, sequenced and localized to chromosome 12p12.1. Within this region, candidate gene sex determining region Y-box 5 (SOX5) was proposed. Further studies of SOX5 in cell lines, xenografts and human prostate specimens, at both the RNA and protein levels, found overexpression of the gene in tumors. This overexpression was then subsequently found by fluorescent in situ hybridization to be caused by amplification of the region. In conclusion, our results suggest LCM coupled with SMAL-PCR DNA fingerprinting is a useful method for the screening and identification of chromosomal regions and genes associated with cancer development. Further, overexpression of SOX5 is associated with prostate tumor progression and early development of distant metastasis. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/58247
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
Funding AgencyGrant Number
The University of Hong Kong Small Project20070717607
British Columbia Cancer Foundation, Department of Defense (USA)
Genorne British Columbia/ Canada, Health Canada
Funding Information:

Grant sponsor: The University of Hong Kong Small Project Funding; Grant number: 20070717607; Grant sponsors: British Columbia Cancer Foundation, Department of Defense (USA), Genorne British Columbia/ Canada, Health Canada.

References

 

DC FieldValueLanguage
dc.contributor.authorMa, Sen_HK
dc.contributor.authorYuen, PCen_HK
dc.contributor.authorWoolcock, Ben_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorKai, YWen_HK
dc.contributor.authorMing, TLen_HK
dc.contributor.authorBainbridge, Ten_HK
dc.contributor.authorWebber, Den_HK
dc.contributor.authorChan, THMen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorLam, Wen_HK
dc.contributor.authorVielkind, Jen_HK
dc.contributor.authorKwok, WCen_HK
dc.date.accessioned2010-05-31T03:26:34Z-
dc.date.available2010-05-31T03:26:34Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Cancer, 2009, v. 124 n. 10, p. 2323-2332en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58247-
dc.description.abstractIdentification of genomic alterations associated with the progression of prostate cancer may facilitate the better understanding of the development of this highly variable disease. Matched normal, premalignant high-grade prostatic intraepithelial neoplasia and invasive prostate carcinoma cells were procured by laser capture microdissection (LCM) from human radical prostatectomy specimens. From these cells, comparative DNA fingerprints were generated by a modified PCR-based technique called scanning of microdissected archival lesion (SMAL)-PCR. Recurrent polymorphic fingerprint fragments were used in tagging altered chromosomal regions. Altered regions were found at cytobands 1p31.3, 1q44, 2p23.1, 3p26.3, 3q22.3, 4q22.3, 4q35.2, 5q23.2, 8q22.3, 8q24.13, 9q21.3, 9q22.32, 10q11.21, 11p13, 12p12.1, 13q12.1, 16q12.2 and 18q21.31. Candidate genes in the surrounding area that may possibly harbor mutations that change normal prostatic cells to progress into their tumor stages were proposed. Of these fragments, a 420 bp alteration, absent in all 26 normal samples screened, was observed in 2 tumors. This fragment was cloned, sequenced and localized to chromosome 12p12.1. Within this region, candidate gene sex determining region Y-box 5 (SOX5) was proposed. Further studies of SOX5 in cell lines, xenografts and human prostate specimens, at both the RNA and protein levels, found overexpression of the gene in tumors. This overexpression was then subsequently found by fluorescent in situ hybridization to be caused by amplification of the region. In conclusion, our results suggest LCM coupled with SMAL-PCR DNA fingerprinting is a useful method for the screening and identification of chromosomal regions and genes associated with cancer development. Further, overexpression of SOX5 is associated with prostate tumor progression and early development of distant metastasis. © 2008 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectChromosome 12p12.1en_HK
dc.subjectLCMen_HK
dc.subjectProstate canceren_HK
dc.subjectSMAL-PCR DNA fingerprintingen_HK
dc.subjectSOX5en_HK
dc.titleDNA fingerprinting tags novel altered chromosomal regions and identifies the involvement of SOX5 in the progression of prostate canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=124&spage=2323&epage=2332&date=2009&atitle=DNA+fingerprinting+tags+novel+altered+chromosomal+regions+and+identifies+the+involvement+of+SOX5+in+the+progression+of+prostate+canceren_HK
dc.identifier.emailMa, S:sma@pathology.hku.hken_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityMa, S=rp00506en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/ijc.24243en_HK
dc.identifier.scopuseid_2-s2.0-64249173250en_HK
dc.identifier.hkuros158603en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64249173250&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume124en_HK
dc.identifier.issue10en_HK
dc.identifier.spage2323en_HK
dc.identifier.epage2332en_HK
dc.identifier.isiWOS:000265353200008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMa, S=16444895800en_HK
dc.identifier.scopusauthoridYuen, PC=26868290000en_HK
dc.identifier.scopusauthoridWoolcock, B=7801355393en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridKai, YW=26867740400en_HK
dc.identifier.scopusauthoridMing, TL=26868015600en_HK
dc.identifier.scopusauthoridBainbridge, T=6701527009en_HK
dc.identifier.scopusauthoridWebber, D=7005126214en_HK
dc.identifier.scopusauthoridChan, THM=26431726400en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridLam, W=7203021871en_HK
dc.identifier.scopusauthoridVielkind, J=7004097540en_HK
dc.identifier.scopusauthoridKwok, WC=24171150800en_HK

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