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Article: Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats

TitleNeural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats
Authors
Issue Date2009
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neu
Citation
Journal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80 How to Cite?
Abstract
In the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/58246
ISSN
2013 Impact Factor: 3.968
2013 SCImago Journal Rankings: 2.002
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong Spinal Cord Injury Foundation
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by the University of Hong Kong Spinal Cord Injury Foundation, and grants from the University of Hong Kong and the Hong Kong Research Grants Council (RGC).

References

 

DC FieldValueLanguage
dc.contributor.authorSu, Hen_HK
dc.contributor.authorZhang, Wen_HK
dc.contributor.authorGuo, Jen_HK
dc.contributor.authorGuo, Aen_HK
dc.contributor.authorYuan, Qen_HK
dc.contributor.authorWu, Wen_HK
dc.date.accessioned2010-05-31T03:26:33Z-
dc.date.available2010-05-31T03:26:33Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80en_HK
dc.identifier.issn0897-7151en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58246-
dc.description.abstractIn the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neuen_HK
dc.relation.ispartofJournal of Neurotraumaen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Survival - physiologyen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGraft Survival - physiologyen_HK
dc.subject.meshMotor Neuron Disease - physiopathology - therapyen_HK
dc.subject.meshMotor Neurons - physiologyen_HK
dc.subject.meshNerve Growth Factors - genetics - secretionen_HK
dc.subject.meshNerve Regeneration - physiologyen_HK
dc.subject.meshPeripheral Nerves - cytology - physiology - transplantationen_HK
dc.subject.meshRadiculopathy - physiopathology - therapyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshRats, Transgenicen_HK
dc.subject.meshRecovery of Function - physiologyen_HK
dc.subject.meshSpinal Cord - pathology - physiopathology - surgeryen_HK
dc.subject.meshStem Cell Transplantation - methodsen_HK
dc.subject.meshStem Cells - cytology - physiologyen_HK
dc.subject.meshTissue Transplantation - methodsen_HK
dc.subject.meshTreatment Outcomeen_HK
dc.titleNeural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0897-7151&volume=26&issue=1&spage=67&epage=80&date=2009&atitle=Neural+progenitor+cells+enhance+the+survival+and+axonal+regeneration+of+injured+motoneurons+after+transplantation+into+the+avulsed+ventral+horn+of+adult+ratsen_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1089/neu.2008.0656en_HK
dc.identifier.pmid19196181en_HK
dc.identifier.scopuseid_2-s2.0-59849127305en_HK
dc.identifier.hkuros163966en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-59849127305&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage67en_HK
dc.identifier.epage80en_HK
dc.identifier.isiWOS:000263516700006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSu, H=16317750200en_HK
dc.identifier.scopusauthoridZhang, W=9044147100en_HK
dc.identifier.scopusauthoridGuo, J=7404488603en_HK
dc.identifier.scopusauthoridGuo, A=55137882000en_HK
dc.identifier.scopusauthoridYuan, Q=7202814773en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK

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