Article: Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats
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- Publisher Website: 10.1089/neu.2008.0656
- Scopus: eid_2-s2.0-59849127305
- PMID: 19196181
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| Title | Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Su, H Zhang, W Guo, J Guo, A Yuan, Q Wu, W1 | ||||||
| Issue Date | 2009 | ||||||
| Publisher | Mary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neu | ||||||
| Citation | Journal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80 [How to Cite?] DOI: http://dx.doi.org/10.1089/neu.2008.0656 | ||||||
| Abstract | In the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc. | ||||||
| ISSN | 0897-7151 2011 Impact Factor: 3.654 2011 SCImago Journal Rankings: 0.275 | ||||||
| DOI | http://dx.doi.org/10.1089/neu.2008.0656 | ||||||
| ISI Accession Number ID | WOS:000263516700006
Funding Information: This study was supported by the University of Hong Kong Spinal Cord Injury Foundation, and grants from the University of Hong Kong and the Hong Kong Research Grants Council (RGC). | ||||||
| References | References in Scopus |
| dc.contributor.author | Su, H | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Zhang, W | ||||||
| dc.contributor.author | Guo, J | ||||||
| dc.contributor.author | Guo, A | ||||||
| dc.contributor.author | Yuan, Q | ||||||
| dc.contributor.author | Wu, W | ||||||
| dc.date.accessioned | 2010-05-31T03:26:33Z | ||||||
| dc.date.available | 2010-05-31T03:26:33Z | ||||||
| dc.date.issued | 2009 | ||||||
| dc.description.abstract | In the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Journal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80 [How to Cite?] DOI: http://dx.doi.org/10.1089/neu.2008.0656 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1089/neu.2008.0656 | ||||||
| dc.identifier.epage | 80 | ||||||
| dc.identifier.hkuros | 163966 | ||||||
| dc.identifier.isi | WOS:000263516700006
Funding Information: This study was supported by the University of Hong Kong Spinal Cord Injury Foundation, and grants from the University of Hong Kong and the Hong Kong Research Grants Council (RGC). | ||||||
| dc.identifier.issn | 0897-7151 2011 Impact Factor: 3.654 2011 SCImago Journal Rankings: 0.275 | ||||||
| dc.identifier.issue | 1 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 19196181 | ||||||
| dc.identifier.scopus | eid_2-s2.0-59849127305 | ||||||
| dc.identifier.spage | 67 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/58246 | ||||||
| dc.identifier.volume | 26 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Mary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neu | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Journal of Neurotrauma | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Animals | ||||||
| dc.subject.mesh | Cell Survival - physiology | ||||||
| dc.subject.mesh | Disease Models, Animal | ||||||
| dc.subject.mesh | Female | ||||||
| dc.subject.mesh | Graft Survival - physiology | ||||||
| dc.subject.mesh | Motor Neuron Disease - physiopathology - therapy | ||||||
| dc.subject.mesh | Motor Neurons - physiology | ||||||
| dc.subject.mesh | Nerve Growth Factors - genetics - secretion | ||||||
| dc.subject.mesh | Nerve Regeneration - physiology | ||||||
| dc.subject.mesh | Peripheral Nerves - cytology - physiology - transplantation | ||||||
| dc.subject.mesh | Radiculopathy - physiopathology - therapy | ||||||
| dc.subject.mesh | Rats | ||||||
| dc.subject.mesh | Rats, Sprague-Dawley | ||||||
| dc.subject.mesh | Rats, Transgenic | ||||||
| dc.subject.mesh | Recovery of Function - physiology | ||||||
| dc.subject.mesh | Spinal Cord - pathology - physiopathology - surgery | ||||||
| dc.subject.mesh | Stem Cell Transplantation - methods | ||||||
| dc.subject.mesh | Stem Cells - cytology - physiology | ||||||
| dc.subject.mesh | Tissue Transplantation - methods | ||||||
| dc.subject.mesh | Treatment Outcome | ||||||
| dc.title | Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong Li Ka Shing Faculty of Medicine