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Article: Neural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats
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TitleNeural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats
 
AuthorsSu, H
Zhang, W
Guo, J
Guo, A
Yuan, Q
Wu, W1
 
Issue Date2009
 
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neu
 
CitationJournal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80 [How to Cite?]
DOI: http://dx.doi.org/10.1089/neu.2008.0656
 
AbstractIn the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc.
 
ISSN0897-7151
2013 Impact Factor: 3.968
2013 SCImago Journal Rankings: 2.002
 
DOIhttp://dx.doi.org/10.1089/neu.2008.0656
 
ISI Accession Number IDWOS:000263516700006
Funding AgencyGrant Number
University of Hong Kong Spinal Cord Injury Foundation
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by the University of Hong Kong Spinal Cord Injury Foundation, and grants from the University of Hong Kong and the Hong Kong Research Grants Council (RGC).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSu, H
 
dc.contributor.authorZhang, W
 
dc.contributor.authorGuo, J
 
dc.contributor.authorGuo, A
 
dc.contributor.authorYuan, Q
 
dc.contributor.authorWu, W
 
dc.date.accessioned2010-05-31T03:26:33Z
 
dc.date.available2010-05-31T03:26:33Z
 
dc.date.issued2009
 
dc.description.abstractIn the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo. © Copyright 2009, Mary Ann Liebert, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Neurotrauma, 2009, v. 26 n. 1, p. 67-80 [How to Cite?]
DOI: http://dx.doi.org/10.1089/neu.2008.0656
 
dc.identifier.doihttp://dx.doi.org/10.1089/neu.2008.0656
 
dc.identifier.epage80
 
dc.identifier.hkuros163966
 
dc.identifier.isiWOS:000263516700006
Funding AgencyGrant Number
University of Hong Kong Spinal Cord Injury Foundation
Hong Kong Research Grants Council (RGC)
Funding Information:

This study was supported by the University of Hong Kong Spinal Cord Injury Foundation, and grants from the University of Hong Kong and the Hong Kong Research Grants Council (RGC).

 
dc.identifier.issn0897-7151
2013 Impact Factor: 3.968
2013 SCImago Journal Rankings: 2.002
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid19196181
 
dc.identifier.scopuseid_2-s2.0-59849127305
 
dc.identifier.spage67
 
dc.identifier.urihttp://hdl.handle.net/10722/58246
 
dc.identifier.volume26
 
dc.languageeng
 
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/neu
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Neurotrauma
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCell Survival - physiology
 
dc.subject.meshDisease Models, Animal
 
dc.subject.meshFemale
 
dc.subject.meshGraft Survival - physiology
 
dc.subject.meshMotor Neuron Disease - physiopathology - therapy
 
dc.subject.meshMotor Neurons - physiology
 
dc.subject.meshNerve Growth Factors - genetics - secretion
 
dc.subject.meshNerve Regeneration - physiology
 
dc.subject.meshPeripheral Nerves - cytology - physiology - transplantation
 
dc.subject.meshRadiculopathy - physiopathology - therapy
 
dc.subject.meshRats
 
dc.subject.meshRats, Sprague-Dawley
 
dc.subject.meshRats, Transgenic
 
dc.subject.meshRecovery of Function - physiology
 
dc.subject.meshSpinal Cord - pathology - physiopathology - surgery
 
dc.subject.meshStem Cell Transplantation - methods
 
dc.subject.meshStem Cells - cytology - physiology
 
dc.subject.meshTissue Transplantation - methods
 
dc.subject.meshTreatment Outcome
 
dc.titleNeural progenitor cells enhance the survival and axonal regeneration of injured motoneurons after transplantation into the avulsed ventral horn of adult rats
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine