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Article: In vitro-matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution

TitleIn vitro-matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistribution
Authors
Keywordsdevelopment
in vitro maturation
Mitochondria
oocyte
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2009, v. 91 n. 3, p. 900-907 How to Cite?
AbstractObjective: To study the development and function of mitochondria in in vitro-matured rat oocytes derived from follicles of different sizes. Design: Experimental animal study. Setting: Department of Anatomy at the University of Hong Kong. Animal(s): Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s): Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s): The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s): The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro-matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo-matured oocytes. Compared with in vitro-matured oocytes from small antral follicles, those from preovulatory follicles and in vivo-matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s): The inferior developmental potential of in vitro-matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events. © 2009 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/58227
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
Funding AgencyGrant Number
Committee on Research and Conference Grants at the University of Hong Kong10207287.03678.20200. 323.01
Funding Information:

Supported by a grant from the Committee on Research and Conference Grants at the University of Hong Kong (10207287.03678.20200. 323.01; Hong Kong, Special Administrative Region, China).

References

 

DC FieldValueLanguage
dc.contributor.authorZeng, Hten_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorCheung, MPLen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorLee, CKFen_HK
dc.contributor.authorZhuang, Glen_HK
dc.contributor.authorLiang, Xyen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-05-31T03:26:12Z-
dc.date.available2010-05-31T03:26:12Z-
dc.date.issued2009en_HK
dc.identifier.citationFertility And Sterility, 2009, v. 91 n. 3, p. 900-907en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58227-
dc.description.abstractObjective: To study the development and function of mitochondria in in vitro-matured rat oocytes derived from follicles of different sizes. Design: Experimental animal study. Setting: Department of Anatomy at the University of Hong Kong. Animal(s): Immature female Sprague-Dawley rats that were 25 days of age. Intervention(s): Immature oocytes were collected from rat ovarian follicles of different sizes and were induced to mature in vitro. Main Outcome Measure(s): The number of copies of mitochondrial DNA, mitochondrial activity, adenosine triphosphate content of matured oocytes, and rates of fertilization and blastulation were determined. Result(s): The mitochondrial DNA copy number of oocytes increased linearly with the diameter of antral follicles. The mitochondrial DNA copy number, adenosine triphosphate content, and proportion of oocytes with peripheral distribution of mitochondria in in vitro-matured oocytes from small antral follicles were significantly lower than those from preovulatory follicles and in vivo-matured oocytes. Compared with in vitro-matured oocytes from small antral follicles, those from preovulatory follicles and in vivo-matured oocytes also had significantly better fertilization potential and higher blastulation rate. Conclusion(s): The inferior developmental potential of in vitro-matured oocytes may be attributed partly to a reduced number of mitochondria, resulting in insufficient production of adenosine triphosphate for required developmental events. © 2009 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectdevelopmenten_HK
dc.subjectin vitro maturationen_HK
dc.subjectMitochondriaen_HK
dc.subjectoocyteen_HK
dc.subject.meshAdenosine Triphosphate - metabolismen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBlastula - metabolismen_HK
dc.subject.meshCarbonyl Cyanide p-Trifluoromethoxyphenylhydrazone - pharmacologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDNA, Mitochondrial - metabolismen_HK
dc.subject.meshEmbryo Culture Techniquesen_HK
dc.subject.meshEmbryonic Developmenten_HK
dc.subject.meshEnergy Metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFertilization in Vitroen_HK
dc.subject.meshMitochondria - drug effects - metabolismen_HK
dc.subject.meshOocytes - drug effects - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshUncoupling Agents - pharmacologyen_HK
dc.titleIn vitro-matured rat oocytes have low mitochondrial deoxyribonucleic acid and adenosine triphosphate contents and have abnormal mitochondrial redistributionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=91 No. 3&spage=900&epage=907&date=2009&atitle=In+vitro-matured+rat+oocytes+have+low+mitochondrial+deoxyribonucleic+acid+and+adenosine+triphosphate+contents+and+have+abnormal+mitochondrial+redistributionen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailLee, CKF:ckflee@hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityLee, CKF=rp00458en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fertnstert.2007.12.008en_HK
dc.identifier.pmid18321496-
dc.identifier.scopuseid_2-s2.0-61449218779en_HK
dc.identifier.hkuros155111en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61449218779&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue3en_HK
dc.identifier.spage900en_HK
dc.identifier.epage907en_HK
dc.identifier.isiWOS:000264143500037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZeng, Ht=16837514100en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridCheung, MPL=16749051200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridLee, CKF=26643097500en_HK
dc.identifier.scopusauthoridZhuang, Gl=7004628287en_HK
dc.identifier.scopusauthoridLiang, Xy=7401735965en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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