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Article: Increased fetal abortion rate in autoimmune thyroid disease is related to circulating TPO autoantibodies in an autoimmune thyroiditis animal model

TitleIncreased fetal abortion rate in autoimmune thyroid disease is related to circulating TPO autoantibodies in an autoimmune thyroiditis animal model
Authors
Keywordsabortion
Autoimmune thyroiditis
implantation rate
preimplantation embryo development
thyroid peroxidase
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2009, v. 91 n. 5 SUPPL., p. 2104-2109 How to Cite?
Abstract
Objective: To determine the fertility and abortion rates in a mouse model of autoimmune thyroiditis and its relationship with circulating anti-thyroid peroxidase (TPO) antibody. Design: Experimental animal study. Setting: University research laboratory. Animal(s): C57bl/6 mice. Intervention(s): Female C57bl/6 mice immunized with recombinant mouse TPO (rmTPO) in complete Freund adjuvant (CFA) or glutathione-S-transferase (GST-CFA) were allowed to mate. The pregnant mice were killed on day 14 of pregnancy for assessment of fetal development. The effects of TPO antibody on preimplantation embryo development and implantation rate were also studied. Main Outcome Measure(s): Litter size, resorption rate, preimplantation embryo development, and implantation rate. Result(s): All of the mice immunized with rmTPO-CFA possessed anti-TPO antibody. They had reduced litter size and increased incidence of resorbed fetus compared with the control. Higher serum TSH levels, but not T 4 levels, were demonstrated after rmTPO-CFA immunization. Anti-TPO antibody bound to preimplantation embryos. Treatment of the embryos with the antibody marginally decreased the formation of 3/4-cell embryos but had no effect on the subsequent development and implantation compared with the nonimmune control sera. Conclusion(s): Autoimmune thyroiditis is associated with reduced fertility and higher incidence of fetal loss. The anti-TPO antibody may affect post-implantation embryo development, leading to fetal loss. © 2009 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/58225
ISSN
2013 Impact Factor: 4.295
2013 SCImago Journal Rankings: 1.712
ISI Accession Number ID
Funding AgencyGrant Number
Committee on Research and Conference Grants (CRCG)
Endocrine and Osteoporosis Research Fund, University of Hong Kong
Funding Information:

Supported by Committee on Research and Conference Grants (CRCG) grant and Endocrine and Osteoporosis Research Fund, University of Hong Kong.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorLee, YLen_HK
dc.contributor.authorNg, HPen_HK
dc.contributor.authorLau, KSen_HK
dc.contributor.authorLiu, WMen_HK
dc.contributor.authorO, WSen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorKung, AWCen_HK
dc.date.accessioned2010-05-31T03:26:10Z-
dc.date.available2010-05-31T03:26:10Z-
dc.date.issued2009en_HK
dc.identifier.citationFertility And Sterility, 2009, v. 91 n. 5 SUPPL., p. 2104-2109en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58225-
dc.description.abstractObjective: To determine the fertility and abortion rates in a mouse model of autoimmune thyroiditis and its relationship with circulating anti-thyroid peroxidase (TPO) antibody. Design: Experimental animal study. Setting: University research laboratory. Animal(s): C57bl/6 mice. Intervention(s): Female C57bl/6 mice immunized with recombinant mouse TPO (rmTPO) in complete Freund adjuvant (CFA) or glutathione-S-transferase (GST-CFA) were allowed to mate. The pregnant mice were killed on day 14 of pregnancy for assessment of fetal development. The effects of TPO antibody on preimplantation embryo development and implantation rate were also studied. Main Outcome Measure(s): Litter size, resorption rate, preimplantation embryo development, and implantation rate. Result(s): All of the mice immunized with rmTPO-CFA possessed anti-TPO antibody. They had reduced litter size and increased incidence of resorbed fetus compared with the control. Higher serum TSH levels, but not T 4 levels, were demonstrated after rmTPO-CFA immunization. Anti-TPO antibody bound to preimplantation embryos. Treatment of the embryos with the antibody marginally decreased the formation of 3/4-cell embryos but had no effect on the subsequent development and implantation compared with the nonimmune control sera. Conclusion(s): Autoimmune thyroiditis is associated with reduced fertility and higher incidence of fetal loss. The anti-TPO antibody may affect post-implantation embryo development, leading to fetal loss. © 2009 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectabortionen_HK
dc.subjectAutoimmune thyroiditisen_HK
dc.subjectimplantation rateen_HK
dc.subjectpreimplantation embryo developmenten_HK
dc.subjectthyroid peroxidaseen_HK
dc.subject.meshAbortion, Spontaneous - epidemiology - etiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAutoantibodies - blooden_HK
dc.subject.meshBlastocyst - immunologyen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshIodide Peroxidase - blood - genetics - immunologyen_HK
dc.subject.meshLitter Sizeen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPregnancy Complications - immunologyen_HK
dc.subject.meshThyroiditis, Autoimmune - immunologyen_HK
dc.subject.meshThyrotropin - blooden_HK
dc.subject.meshThyroxine - blooden_HK
dc.titleIncreased fetal abortion rate in autoimmune thyroid disease is related to circulating TPO autoantibodies in an autoimmune thyroiditis animal modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=91 &issue=5Suppl&spage=2104&epage=2109&date=2008&atitle=Increased+fetal+abortion+rate+in+autoimmune+thyroid+disease+is+related+to+circulating+TPO+autoantibodies+in+an+autoimmune+thyroiditis+animal+model.+en_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailKung, AWC:awckung@hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityKung, AWC=rp00368en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fertnstert.2008.07.1704en_HK
dc.identifier.pmid18774556en_HK
dc.identifier.scopuseid_2-s2.0-65249160685en_HK
dc.identifier.hkuros152583en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65249160685&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue5 SUPPL.en_HK
dc.identifier.spage2104en_HK
dc.identifier.epage2109en_HK
dc.identifier.isiWOS:000265969300018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridNg, HP=14621713300en_HK
dc.identifier.scopusauthoridLau, KS=35205833900en_HK
dc.identifier.scopusauthoridLiu, WM=35548548500en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridKung, AWC=7102322339en_HK

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