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Article: Dietary oxyresveratrol prevents parkinsonian mimetic 6-hydroxydopamine neurotoxicity

TitleDietary oxyresveratrol prevents parkinsonian mimetic 6-hydroxydopamine neurotoxicity
Authors
Keywords6-Hydroxydopamine
Free radicals
Neuroprotection
Oxyresveratrol
Parkinson disease
Resveratrol
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
Free Radical Biology And Medicine, 2008, v. 45 n. 7, p. 1019-1026 How to Cite?
AbstractOxyresveratrol (OXY) is a polyhydroxylated stilbene existing in mulberry. Increasing lines of evidence have shown its neuroprotective effects against Alzheimer disease and stroke. However, little is known about its neuroprotective effect in Parkinson disease (PD). Owing to its antioxidant activity, blood-brain barrier permeativity, and water solubility, we hypothesized that OXY may exert neuroprotective effects against parkinsonian mimetic 6-hydroxydopamine (6-OHDA) neurotoxicity. Neuroblastoma SH-SY5Y cells have long been used as dopaminergic neurons in PD research. We found that both pretreatment and posttreatment with OXY on SH-SY5Y cells significantly reduced the release of lactate dehydrogenase, the activity of caspase-3, and the generation of intracellular reactive oxygen species triggered by 6-OHDA. Compared to resveratrol, OXY exhibited a wider effective dosage range. We proved that OXY could penetrate the cell membrane by HPLC analysis of cell extracts. These results suggest that OXY may act as an intracellular antioxidant to reduce oxidative stress induced by 6-OHDA. Western blot analysis demonstrated that OXY markedly attenuated 6-OHDA-induced phosphorylation of JNK and c-Jun. Furthermore, we proved that OXY increased the basal levels of SIRT1, which may disclose new pathways accounting for the neuroprotective effects of OXY. Taken together, our results suggest OXY, a dietary phenolic compound, as a potential nutritional candidate for protection against neurodegeneration in PD. © 2008 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58221
ISSN
2015 Impact Factor: 5.784
2015 SCImago Journal Rankings: 2.468
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChao, Jen_HK
dc.contributor.authorYu, MSen_HK
dc.contributor.authorHo, YSen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorChang, RCCen_HK
dc.date.accessioned2010-05-31T03:26:05Z-
dc.date.available2010-05-31T03:26:05Z-
dc.date.issued2008en_HK
dc.identifier.citationFree Radical Biology And Medicine, 2008, v. 45 n. 7, p. 1019-1026en_HK
dc.identifier.issn0891-5849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58221-
dc.description.abstractOxyresveratrol (OXY) is a polyhydroxylated stilbene existing in mulberry. Increasing lines of evidence have shown its neuroprotective effects against Alzheimer disease and stroke. However, little is known about its neuroprotective effect in Parkinson disease (PD). Owing to its antioxidant activity, blood-brain barrier permeativity, and water solubility, we hypothesized that OXY may exert neuroprotective effects against parkinsonian mimetic 6-hydroxydopamine (6-OHDA) neurotoxicity. Neuroblastoma SH-SY5Y cells have long been used as dopaminergic neurons in PD research. We found that both pretreatment and posttreatment with OXY on SH-SY5Y cells significantly reduced the release of lactate dehydrogenase, the activity of caspase-3, and the generation of intracellular reactive oxygen species triggered by 6-OHDA. Compared to resveratrol, OXY exhibited a wider effective dosage range. We proved that OXY could penetrate the cell membrane by HPLC analysis of cell extracts. These results suggest that OXY may act as an intracellular antioxidant to reduce oxidative stress induced by 6-OHDA. Western blot analysis demonstrated that OXY markedly attenuated 6-OHDA-induced phosphorylation of JNK and c-Jun. Furthermore, we proved that OXY increased the basal levels of SIRT1, which may disclose new pathways accounting for the neuroprotective effects of OXY. Taken together, our results suggest OXY, a dietary phenolic compound, as a potential nutritional candidate for protection against neurodegeneration in PD. © 2008 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomeden_HK
dc.relation.ispartofFree Radical Biology and Medicineen_HK
dc.subject6-Hydroxydopamineen_HK
dc.subjectFree radicalsen_HK
dc.subjectNeuroprotectionen_HK
dc.subjectOxyresveratrolen_HK
dc.subjectParkinson diseaseen_HK
dc.subjectResveratrolen_HK
dc.subject.meshAdrenergic Agents - toxicityen_HK
dc.subject.meshAntioxidants - pharmacologyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCaspase 3 - drug effectsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromatography, High Pressure Liquiden_HK
dc.subject.meshHumansen_HK
dc.subject.meshL-Lactate Dehydrogenase - drug effectsen_HK
dc.subject.meshNeurons - drug effectsen_HK
dc.subject.meshNeuroprotective Agents - pharmacologyen_HK
dc.subject.meshOxidative Stress - drug effectsen_HK
dc.subject.meshOxidopamine - toxicityen_HK
dc.subject.meshPlant Extracts - pharmacologyen_HK
dc.subject.meshReactive Oxygen Species - metabolismen_HK
dc.subject.meshStilbenes - pharmacologyen_HK
dc.titleDietary oxyresveratrol prevents parkinsonian mimetic 6-hydroxydopamine neurotoxicityen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailChang, RCC: rccchang@hku.hken_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityChang, RCC=rp00470en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.freeradbiomed.2008.07.002en_HK
dc.identifier.pmid18675900-
dc.identifier.scopuseid_2-s2.0-51549098145en_HK
dc.identifier.hkuros148391en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51549098145&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1019en_HK
dc.identifier.epage1026en_HK
dc.identifier.eissn1873-4596-
dc.identifier.isiWOS:000259667000009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChao, J=24558959000en_HK
dc.identifier.scopusauthoridYu, MS=35346047600en_HK
dc.identifier.scopusauthoridHo, YS=14031513600en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridChang, RCC=7403713410en_HK

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