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Article: Molecular cloning and characterization of the zebrafish (Danio rerio) telomerase catalytic subunit (telomerase reverse transcriptase, TERT)

TitleMolecular cloning and characterization of the zebrafish (Danio rerio) telomerase catalytic subunit (telomerase reverse transcriptase, TERT)
Authors
KeywordsCloning
Immunohistochemistry
Rapid amplification of cDNA ends
RT-PCR
Telomerase
Telomerase reverse transcriptase
TRAP assay
Zebrafish
Issue Date2008
PublisherHumana Press, Inc
Citation
Journal Of Molecular Neuroscience, 2008, v. 34 n. 1, p. 63-75 How to Cite?
AbstractTelomerase is an enzyme composed of a catalytic subunit (TERT) and RNA template (TR), which specifically elongates telomeres and prevents cellular senescence. Although telomerase cannot be detected in most human somatic tissues, including the nervous system, it can be detected in teleost tissues. To facilitate the investigation of telomerase function in the teleost visual system, the coding sequence of zebrafish TERT is revealed and cloned. Immunoblot, immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR), and telomeric repeats amplification protocol (TRAP) assay are used to assess the expression of telomerase at mRNA, protein, and functional levels in zebrafish retina. Based on the amino acid sequence of mouse TERT, a full-length telomerase reverse transcriptase cDNA of zebrafish has been isolated and cloned. The deduced protein sequence contains 1,091 amino acid residues and a predicted molecular mass of 126 kDa. Multiple alignment shows that the protein sequence contains the conserved motifs and residues found in TERT of other species. RT-PCR and TRAP assay has detected TERT mRNA expression and telomerase activity, respectively, in all tissues examined, including the retina and the brain. The presence of telomerase activity indicates that a fully functional form of telomerase can be found in the retina. Immunohistochemistry reveals that most neurons in zebrafish retina express TERT in the cell nucleus. The presence of telomerase in different tissues may be associated with the indeterminate growth of teleost. However, teleost retinal neurons are post-mitotic and do not further divide under normal situation. The expression of telomerase activity and TERT in retina implies that telomerase has functions other than the elongation of telomere. These findings could provide new insights on telomerase function in the nervous system. © 2007 Humana Press Inc.
Persistent Identifierhttp://hdl.handle.net/10722/58200
ISSN
2015 Impact Factor: 2.352
2015 SCImago Journal Rankings: 0.988
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, BWMen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorTsao, GSWen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorYip, HKFen_HK
dc.date.accessioned2010-05-31T03:25:42Z-
dc.date.available2010-05-31T03:25:42Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Molecular Neuroscience, 2008, v. 34 n. 1, p. 63-75en_HK
dc.identifier.issn0895-8696en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58200-
dc.description.abstractTelomerase is an enzyme composed of a catalytic subunit (TERT) and RNA template (TR), which specifically elongates telomeres and prevents cellular senescence. Although telomerase cannot be detected in most human somatic tissues, including the nervous system, it can be detected in teleost tissues. To facilitate the investigation of telomerase function in the teleost visual system, the coding sequence of zebrafish TERT is revealed and cloned. Immunoblot, immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR), and telomeric repeats amplification protocol (TRAP) assay are used to assess the expression of telomerase at mRNA, protein, and functional levels in zebrafish retina. Based on the amino acid sequence of mouse TERT, a full-length telomerase reverse transcriptase cDNA of zebrafish has been isolated and cloned. The deduced protein sequence contains 1,091 amino acid residues and a predicted molecular mass of 126 kDa. Multiple alignment shows that the protein sequence contains the conserved motifs and residues found in TERT of other species. RT-PCR and TRAP assay has detected TERT mRNA expression and telomerase activity, respectively, in all tissues examined, including the retina and the brain. The presence of telomerase activity indicates that a fully functional form of telomerase can be found in the retina. Immunohistochemistry reveals that most neurons in zebrafish retina express TERT in the cell nucleus. The presence of telomerase in different tissues may be associated with the indeterminate growth of teleost. However, teleost retinal neurons are post-mitotic and do not further divide under normal situation. The expression of telomerase activity and TERT in retina implies that telomerase has functions other than the elongation of telomere. These findings could provide new insights on telomerase function in the nervous system. © 2007 Humana Press Inc.en_HK
dc.languageengen_HK
dc.publisherHumana Press, Incen_HK
dc.relation.ispartofJournal of Molecular Neuroscienceen_HK
dc.subjectCloningen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectRapid amplification of cDNA endsen_HK
dc.subjectRT-PCRen_HK
dc.subjectTelomeraseen_HK
dc.subjectTelomerase reverse transcriptaseen_HK
dc.subjectTRAP assayen_HK
dc.subjectZebrafishen_HK
dc.subject.meshAmino Acid Sequence - geneticsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequence - geneticsen_HK
dc.subject.meshBrain - cytology - metabolismen_HK
dc.subject.meshCell Differentiation - geneticsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshConserved Sequenceen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshMolecular Weighten_HK
dc.subject.meshNeurons - metabolismen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshRNA, Messenger - analysis - genetics - metabolismen_HK
dc.subject.meshRetina - cytology - metabolismen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshTelomerase - genetics - isolation & purification - metabolismen_HK
dc.subject.meshZebrafish - genetics - growth & development - metabolismen_HK
dc.subject.meshZebrafish Proteins - genetics - isolation & purification - metabolismen_HK
dc.titleMolecular cloning and characterization of the zebrafish (Danio rerio) telomerase catalytic subunit (telomerase reverse transcriptase, TERT)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0895-8696&volume=34&spage=63&epage=75&date=2008&atitle=Molecular+cloning+and+characterization+of+the+zebrafish+(Danio+rerio)+telomerase+catalytic+subunit+(telomerase+reverse+transcriptase,+TERT).en_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.emailTsao, GSW: gswtsao@hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hku.hken_HK
dc.identifier.emailYip, HKF: hkfyip@hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityYip, HKF=rp00285en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s12031-007-0072-xen_HK
dc.identifier.pmid18157659-
dc.identifier.scopuseid_2-s2.0-40749088495en_HK
dc.identifier.hkuros151299en_HK
dc.identifier.hkuros141344-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40749088495&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue1en_HK
dc.identifier.spage63en_HK
dc.identifier.epage75en_HK
dc.identifier.isiWOS:000251866900008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, BWM=21934562200en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridTsao, GSW=7102813116en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridYip, HKF=7101980864en_HK

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