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Article: Effect of N-acetylcysteine on the early expression of inflammatory markers in the retina and plasma of diabetic rats

TitleEffect of N-acetylcysteine on the early expression of inflammatory markers in the retina and plasma of diabetic rats
Authors
Issue Date2009
PublisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEO
Citation
Clinical And Experimental Ophthalmology, 2009, v. 37 n. 2, p. 223-231 How to Cite?
AbstractPurpose: The aim of this study is to investigate markers of inflammation and oxidative stress in an early model of diabetic retinopathy, correlate retinal and plasma results and evaluate the influence of treatment by N-acetylcysteine (NAC), a free radical scavenger. Methods: Four groups were studied: control (C), streptozotocin (STZ)-induced diabetic rats (D), STZ rats following 8weeks of NAC (DT), and control rats following 8weeks of NAC (CT). Plasma levels of free 15-F2t-isoprostane (15-F-2t-IsoP), superoxide dismutase (SOD) and tumour necrosis factor-alpha (TNF-α) were obtained. Primary antibodies against macrophages (ED-1), microglia (Ox-42), pericytes (NG-2), endothelial and perivascular cells (IB-4), haem oxygenase 1 (HO-1) and vascular endothelial growth factor (VEGF) were used. Results: Expression of NG-2 was robust in C, CT, DT, and mild in D. The intensity of IB-4 was higher in D and DT compared with the C and CT. Ox-42 and ED-1 expression was higher in the D than in the DT, C or CT. Expression of VEGF and HO-1 was non-specific across the four groups. Plasma levels of 15-F-2t-IsoP and TNF-α were higher in the D as compared with the C, CT and DT. SOD levels were lower in the D when compared with the C, CT and D. Conclusions: Macrophage/microglia activation, pericyte loss and endothelial/perivascular cell changes occur early in the pathogenesis of DR. These changes are associated with an increase in plasma markers of oxidative stress and inflammation and are minimized by treatment with NAC. The results suggest that therapies that reduce free radicals will help minimize the early events in diabetic retinopathy in the STZ model. © Journal compilation © 2009 Royal Australian and New Zealand College of Ophthalmologists.
Persistent Identifierhttp://hdl.handle.net/10722/58178
ISSN
2015 Impact Factor: 2.546
2015 SCImago Journal Rankings: 1.335
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Canadian Institute for Health Research (CIHR)
Heart and Stroke Foundation
Yukon Program Grant
Funding Information:

The work was supported by the Canadian Institute for Health Research (CIHR) (JAM) and Heart and Stroke Foundation of BC and Yukon Program Grant (JHM). The UBC Faculty of Medicine Summer Student Research Program (GYT) and the CIHR/Rx and D HRF Health Research Foundation Fellowship Program (ZX) also provided funding for this work.

References

 

DC FieldValueLanguage
dc.contributor.authorTsai, GYen_HK
dc.contributor.authorCui, JZen_HK
dc.contributor.authorSyed, Hen_HK
dc.contributor.authorXia, Zen_HK
dc.contributor.authorOzerdem, Uen_HK
dc.contributor.authorMcNeill, JHen_HK
dc.contributor.authorMatsubara, JAen_HK
dc.date.accessioned2010-05-31T03:25:12Z-
dc.date.available2010-05-31T03:25:12Z-
dc.date.issued2009en_HK
dc.identifier.citationClinical And Experimental Ophthalmology, 2009, v. 37 n. 2, p. 223-231en_HK
dc.identifier.issn1442-6404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58178-
dc.description.abstractPurpose: The aim of this study is to investigate markers of inflammation and oxidative stress in an early model of diabetic retinopathy, correlate retinal and plasma results and evaluate the influence of treatment by N-acetylcysteine (NAC), a free radical scavenger. Methods: Four groups were studied: control (C), streptozotocin (STZ)-induced diabetic rats (D), STZ rats following 8weeks of NAC (DT), and control rats following 8weeks of NAC (CT). Plasma levels of free 15-F2t-isoprostane (15-F-2t-IsoP), superoxide dismutase (SOD) and tumour necrosis factor-alpha (TNF-α) were obtained. Primary antibodies against macrophages (ED-1), microglia (Ox-42), pericytes (NG-2), endothelial and perivascular cells (IB-4), haem oxygenase 1 (HO-1) and vascular endothelial growth factor (VEGF) were used. Results: Expression of NG-2 was robust in C, CT, DT, and mild in D. The intensity of IB-4 was higher in D and DT compared with the C and CT. Ox-42 and ED-1 expression was higher in the D than in the DT, C or CT. Expression of VEGF and HO-1 was non-specific across the four groups. Plasma levels of 15-F-2t-IsoP and TNF-α were higher in the D as compared with the C, CT and DT. SOD levels were lower in the D when compared with the C, CT and D. Conclusions: Macrophage/microglia activation, pericyte loss and endothelial/perivascular cell changes occur early in the pathogenesis of DR. These changes are associated with an increase in plasma markers of oxidative stress and inflammation and are minimized by treatment with NAC. The results suggest that therapies that reduce free radicals will help minimize the early events in diabetic retinopathy in the STZ model. © Journal compilation © 2009 Royal Australian and New Zealand College of Ophthalmologists.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CEOen_HK
dc.relation.ispartofClinical and Experimental Ophthalmologyen_HK
dc.subject.meshAcetylcysteine - therapeutic useen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiological Markers - blooden_HK
dc.subject.meshDiabetes Mellitus, Experimental - drug therapy - metabolism - pathologyen_HK
dc.subject.meshDiabetic Retinopathy - drug therapy - metabolism - pathologyen_HK
dc.subject.meshEndothelium, Vascular - metabolism - pathologyen_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshFree Radical Scavengers - therapeutic useen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshInflammation - metabolism - pathologyen_HK
dc.subject.meshIsoprostanes - blooden_HK
dc.subject.meshMacrophages - metabolism - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicroglia - metabolism - pathologyen_HK
dc.subject.meshOxidative Stressen_HK
dc.subject.meshPericytes - metabolism - pathologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Wistaren_HK
dc.subject.meshRetina - drug effects - metabolismen_HK
dc.subject.meshSuperoxide Dismutase - blooden_HK
dc.subject.meshTumor Necrosis Factor-alpha - blooden_HK
dc.titleEffect of N-acetylcysteine on the early expression of inflammatory markers in the retina and plasma of diabetic ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1442-6404&volume=37&spage=223&epage=231&date=2009&atitle=Effect+of+N-acetylcysteine+on+the+Early+Expression+of+Inflammatory+Markers+in+the+Retina+and+Plasma+of+Diabetic+Ratsen_HK
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1442-9071.2009.02000.xen_HK
dc.identifier.pmid19723131-
dc.identifier.pmcidPMC3947378-
dc.identifier.scopuseid_2-s2.0-65449157994en_HK
dc.identifier.hkuros162382en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65449157994&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue2en_HK
dc.identifier.spage223en_HK
dc.identifier.epage231en_HK
dc.identifier.isiWOS:000265407200011-
dc.publisher.placeAustraliaen_HK
dc.identifier.citeulike4401155-

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