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Article: Intrathecal morphine preconditioning induces cardioprotection via activation of delta, kappa, and mu opioid receptors in rats

TitleIntrathecal morphine preconditioning induces cardioprotection via activation of delta, kappa, and mu opioid receptors in rats
Authors
Issue Date2009
PublisherLippincott, Williams & Wilkins. The Journal's web site is located at http://www.anesthesia-analgesia.org
Citation
Anesthesia And Analgesia, 2009, v. 108 n. 1, p. 23-29 How to Cite?
AbstractBACKGROUND:: Small doses of intrathecal morphine provide cardioprotection similar to that conferred by IV morphine. However, the extent of intrathecal morphine preconditioning (IT-MPC) relative to that resulting from ischemic preconditioning (IPC) is unknown. Further, it is uncertain whether IT-MPC is mediated by opioid receptor dependent pathways. In this study, we compared the extent of cardioprotection conferred by IT-MPC with IPC and investigated the role of opioid receptors in this effect. METHODS:: Eighty anesthetized, open-chest, male Sprague-Dawley rats were assigned to 1 of 13 groups (n = 6-7) after successful intrathecal catheter placement. Rats in the IPC group were subjected to three 5-min cycles of myocardial ischemia (induced by occlusion of the left main coronary artery) interspersed with 5 min of reperfusion. After IPC, myocardial ischemia and reperfusion injury was induced by 30 min of left main coronary artery occlusion followed by 2 h of reperfusion. In the IT-MPC groups, the rats were given 3 consecutive 5 min intrathecal morphine infusions (0.03, 0.3, 3.0, or 30.0 μg/kg, respectively) interspersed with 5 min infusion-free periods, before myocardial ischemia reperfusion injury. In 2 other groups either 300μg/kg of IV morphine or 10 μL of intrathecal normal saline were given. The selective delta, kappa, and mu opioid receptor antagonists naltrindole, nor-binaltorphimine, and D-Phe-Cys-Tyr-D-Trp-Orn-Thr- Pen-Thr-NH2 (CTOP), respectively, were given to groups of animals receiving IT-MPC to evaluate the relative role of the specific opioid receptor subtypes in IT-MPC preconditioning. Myocardial infarct size (IS), as a percentage of the area at risk (AAR), was determined by 2,3,5-triphenyltetrazolium staining. RESULTS:: Intrathecal morphine 0.3 to 30 μg/kg reduced myocardial IS compared with intrathecal normal saline control animals. The IS/AAR were 33% ± 10% (0.3 μg/kg), 29% ± 10% (3 μg/kg) and 29% ± 16% (30 μg/kg), versus 53% ± 8% for the control group (P < 0.01). The reduction in IS/AAR with IT-MPC was similar to that produced by IV morphine (33% ± 6%, P = 0.84) and IPC (22% ± 4%, P = 0.41). Myocardial preconditioning due to IT-MPC was attenuated by co-administration of any one of the opioid receptor antagonists (IT-MPC + naltrindole 50% ± 9%, IT-MPC + nor binaltorphimine 43% ± 6%, IT-MPC + CTOP 53% ± 9%, P = 0.14). CONCLUSIONS:: IT-MPC produced comparable cardioprotection to myocardial IPC and IV morphine. Myocardial preconditioning from intrathecal morphine seems to involve delta, kappa, and mu opioid receptors. Copyright © 2008 International Anesthesia Research Society.
DescriptionComment in Anesth Analg. 2010 Apr 1;110(4):1243-1244; author reply 1244.
Persistent Identifierhttp://hdl.handle.net/10722/58174
ISSN
2015 Impact Factor: 3.827
2015 SCImago Journal Rankings: 1.523
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

Supported in part by the Small Project Fund, University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, Ren_HK
dc.contributor.authorWong, GTCen_HK
dc.contributor.authorWong, TMen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorXia, Zen_HK
dc.contributor.authorIrwin, MGen_HK
dc.date.accessioned2010-05-31T03:25:08Z-
dc.date.available2010-05-31T03:25:08Z-
dc.date.issued2009en_HK
dc.identifier.citationAnesthesia And Analgesia, 2009, v. 108 n. 1, p. 23-29en_HK
dc.identifier.issn0003-2999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58174-
dc.descriptionComment in Anesth Analg. 2010 Apr 1;110(4):1243-1244; author reply 1244.-
dc.description.abstractBACKGROUND:: Small doses of intrathecal morphine provide cardioprotection similar to that conferred by IV morphine. However, the extent of intrathecal morphine preconditioning (IT-MPC) relative to that resulting from ischemic preconditioning (IPC) is unknown. Further, it is uncertain whether IT-MPC is mediated by opioid receptor dependent pathways. In this study, we compared the extent of cardioprotection conferred by IT-MPC with IPC and investigated the role of opioid receptors in this effect. METHODS:: Eighty anesthetized, open-chest, male Sprague-Dawley rats were assigned to 1 of 13 groups (n = 6-7) after successful intrathecal catheter placement. Rats in the IPC group were subjected to three 5-min cycles of myocardial ischemia (induced by occlusion of the left main coronary artery) interspersed with 5 min of reperfusion. After IPC, myocardial ischemia and reperfusion injury was induced by 30 min of left main coronary artery occlusion followed by 2 h of reperfusion. In the IT-MPC groups, the rats were given 3 consecutive 5 min intrathecal morphine infusions (0.03, 0.3, 3.0, or 30.0 μg/kg, respectively) interspersed with 5 min infusion-free periods, before myocardial ischemia reperfusion injury. In 2 other groups either 300μg/kg of IV morphine or 10 μL of intrathecal normal saline were given. The selective delta, kappa, and mu opioid receptor antagonists naltrindole, nor-binaltorphimine, and D-Phe-Cys-Tyr-D-Trp-Orn-Thr- Pen-Thr-NH2 (CTOP), respectively, were given to groups of animals receiving IT-MPC to evaluate the relative role of the specific opioid receptor subtypes in IT-MPC preconditioning. Myocardial infarct size (IS), as a percentage of the area at risk (AAR), was determined by 2,3,5-triphenyltetrazolium staining. RESULTS:: Intrathecal morphine 0.3 to 30 μg/kg reduced myocardial IS compared with intrathecal normal saline control animals. The IS/AAR were 33% ± 10% (0.3 μg/kg), 29% ± 10% (3 μg/kg) and 29% ± 16% (30 μg/kg), versus 53% ± 8% for the control group (P < 0.01). The reduction in IS/AAR with IT-MPC was similar to that produced by IV morphine (33% ± 6%, P = 0.84) and IPC (22% ± 4%, P = 0.41). Myocardial preconditioning due to IT-MPC was attenuated by co-administration of any one of the opioid receptor antagonists (IT-MPC + naltrindole 50% ± 9%, IT-MPC + nor binaltorphimine 43% ± 6%, IT-MPC + CTOP 53% ± 9%, P = 0.14). CONCLUSIONS:: IT-MPC produced comparable cardioprotection to myocardial IPC and IV morphine. Myocardial preconditioning from intrathecal morphine seems to involve delta, kappa, and mu opioid receptors. Copyright © 2008 International Anesthesia Research Society.en_HK
dc.languageengen_HK
dc.publisherLippincott, Williams & Wilkins. The Journal's web site is located at http://www.anesthesia-analgesia.orgen_HK
dc.relation.ispartofAnesthesia and Analgesiaen_HK
dc.rightsAnesthesia and Analgesia. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subject.meshAnalgesics, Opioid - administration & dosageen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCardiovascular Agents - administration & dosageen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshHemodynamics - drug effectsen_HK
dc.subject.meshInfusions, Parenteralen_HK
dc.subject.meshIschemic Preconditioning, Myocardialen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMorphine - administration & dosageen_HK
dc.subject.meshMyocardial Infarction - metabolism - pathology - prevention & controlen_HK
dc.subject.meshMyocardial Reperfusion Injury - metabolism - pathology - prevention & controlen_HK
dc.subject.meshMyocardium - pathologyen_HK
dc.subject.meshNarcotic Antagonists - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptors, Opioid - agonists - metabolismen_HK
dc.subject.meshReceptors, Opioid, delta - agonistsen_HK
dc.subject.meshReceptors, Opioid, kappa - agonistsen_HK
dc.subject.meshReceptors, Opioid, mu - agonistsen_HK
dc.titleIntrathecal morphine preconditioning induces cardioprotection via activation of delta, kappa, and mu opioid receptors in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2999&volume=108&issue=1&spage=23&epage=29&date=2009&atitle=Intrathecal+morphine+preconditioning+induces+cardioprotection+via+activation+of+delta,+kappa,+and+mu+opioid+receptors+in+ratsen_HK
dc.identifier.emailWong, GTC:gordon@hku.hken_HK
dc.identifier.emailXia, Z:zyxia@hkucc.hku.hken_HK
dc.identifier.emailIrwin, MG:mgirwin@hku.hken_HK
dc.identifier.authorityWong, GTC=rp00523en_HK
dc.identifier.authorityXia, Z=rp00532en_HK
dc.identifier.authorityIrwin, MG=rp00390en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1213/ane.0b013e3181884ba6en_HK
dc.identifier.pmid19095826en_HK
dc.identifier.scopuseid_2-s2.0-58749085924en_HK
dc.identifier.hkuros160845en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58749085924&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume108en_HK
dc.identifier.issue1en_HK
dc.identifier.spage23en_HK
dc.identifier.epage29en_HK
dc.identifier.eissn1526-7598-
dc.identifier.isiWOS:000261963000006-
dc.publisher.placeUnited Statesen_HK

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