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Article: The assessment of cell cycle genes in the rat mandibular condyle

TitleThe assessment of cell cycle genes in the rat mandibular condyle
Authors
KeywordsCell cycle genes
Cyclin D1
Mandibular condyle
Mechanical strain
PCNA
Wnt5a
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2009, v. 54 n. 5, p. 470-478 How to Cite?
AbstractObjectives: We aimed to investigate the expression profile of cell cycle genes in the mandibular condyle on mechanical strain and natural growth, and quantify their expression intensity. Methods: Three hundred and fifty 35 days old Sprague-Dawley rats were randomly divided into experimental groups fitted with bite-jumping appliances and control groups. Groups were sacrificed at days 1, 3, 7, 9, 14, 30, and 33. Then, condyles were dissected and total RNA was extracted for microarray analysis. Results: Thirty-nine known cell cycle genes were present in the condyle, where Cyclin D1, PCNA, and Wnt5a were differentially expressed. Reverse transcriptase-PCR confirmed that Wnt5a showed a 2-fold increase on experimental day 1, Cyclin D1 showed a 2-fold increase on experimental day 1 and a 3-fold increase on experimental day 14, while PCNA shows 2.2-fold increase both on experimental days 9 and 30. PCNA, Cyclin D1, and Wnt5a were all expressed by cells in both the proliferative layer and erosive zone. Conclusion: Mandibular advancement leads to the expression of Cyclin D1 that accelerates entry to the S phase. The increased level of PCNA indicates increased DNA replication of MSC. Then, elevated level of Wnt5a indicates the commitment of MSC to the chondrogenic lineage. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58159
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
Funding AgencyGrant Number
V. Leung Foundation
Funding Information:

The present study was supported by The V. Leung Foundation which was established by a generous donation from Dr. Vincent Leung and his wife Mrs. Marianne Leung.

References

 

DC FieldValueLanguage
dc.contributor.authorAlkalaly, Aen_HK
dc.contributor.authorWu, Cen_HK
dc.contributor.authorWong, Ren_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-05-31T03:24:48Z-
dc.date.available2010-05-31T03:24:48Z-
dc.date.issued2009en_HK
dc.identifier.citationArchives Of Oral Biology, 2009, v. 54 n. 5, p. 470-478en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58159-
dc.description.abstractObjectives: We aimed to investigate the expression profile of cell cycle genes in the mandibular condyle on mechanical strain and natural growth, and quantify their expression intensity. Methods: Three hundred and fifty 35 days old Sprague-Dawley rats were randomly divided into experimental groups fitted with bite-jumping appliances and control groups. Groups were sacrificed at days 1, 3, 7, 9, 14, 30, and 33. Then, condyles were dissected and total RNA was extracted for microarray analysis. Results: Thirty-nine known cell cycle genes were present in the condyle, where Cyclin D1, PCNA, and Wnt5a were differentially expressed. Reverse transcriptase-PCR confirmed that Wnt5a showed a 2-fold increase on experimental day 1, Cyclin D1 showed a 2-fold increase on experimental day 1 and a 3-fold increase on experimental day 14, while PCNA shows 2.2-fold increase both on experimental days 9 and 30. PCNA, Cyclin D1, and Wnt5a were all expressed by cells in both the proliferative layer and erosive zone. Conclusion: Mandibular advancement leads to the expression of Cyclin D1 that accelerates entry to the S phase. The increased level of PCNA indicates increased DNA replication of MSC. Then, elevated level of Wnt5a indicates the commitment of MSC to the chondrogenic lineage. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectCell cycle genesen_HK
dc.subjectCyclin D1en_HK
dc.subjectMandibular condyleen_HK
dc.subjectMechanical strainen_HK
dc.subjectPCNAen_HK
dc.subjectWnt5aen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshCyclin D1 - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGenes, bcl-1 - geneticsen_HK
dc.subject.meshGenes, cdcen_HK
dc.subject.meshMandibular Advancement - instrumentationen_HK
dc.subject.meshMandibular Condyle - cytology - growth & development - physiologyen_HK
dc.subject.meshMesoderm - cytologyen_HK
dc.subject.meshMicroarray Analysisen_HK
dc.subject.meshOrthodontic Appliancesen_HK
dc.subject.meshProliferating Cell Nuclear Antigen - geneticsen_HK
dc.subject.meshRandom Allocationen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshS Phase - geneticsen_HK
dc.subject.meshStress, Mechanicalen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshWnt Proteins - geneticsen_HK
dc.titleThe assessment of cell cycle genes in the rat mandibular condyleen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=54&spage=470&epage=478&date=2009&atitle=The+assessment+of+cell+cycle+genes+in+the+rat+mandibular+condyleen_HK
dc.identifier.emailWong, R: fyoung@hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityWong, R=rp00038en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2009.01.020en_HK
dc.identifier.pmid19264292-
dc.identifier.scopuseid_2-s2.0-63549113278en_HK
dc.identifier.hkuros158866en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63549113278&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue5en_HK
dc.identifier.spage470en_HK
dc.identifier.epage478en_HK
dc.identifier.isiWOS:000265736100012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridAlkalaly, A=24922819100en_HK
dc.identifier.scopusauthoridWu, C=7501667713en_HK
dc.identifier.scopusauthoridWong, R=7402127170en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK
dc.identifier.citeulike4807953-

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