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Article: Improvement of XTT assay performance for studies involving Candida albicans biofilms

TitleImprovement of XTT assay performance for studies involving Candida albicans biofilms
Authors
KeywordsBiofilm
Candida albicans
XTT
Issue Date2008
PublisherFundacao Odontologica de Ribeirao Preto. The Journal's web site is located at http://www.forp.usp.br/bdj/bdj.htm
Citation
Brazilian Dental Journal, 2008, v. 19 n. 4, p. 364-369 How to Cite?
Abstract2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm bottom cell layers tend to be relatively quiescent at later stages of biofilm formation. The aim of this study was to improve XTT reduction assay by adding glucose supplements to the standard XTT formulation. Candida albicans ATCC 90028 was used to form 24-, 48- and 72-h biofilms. The oxidative activity at 90, 180 and 270 min of incubation was evaluated. The control consisted of standard XTT formulation without glucose supplements, and was modified by the addition of 50, 100 and 200 mM of glucose. The XTT assay with 200 mM glucose showed more accurate and consistent readings correlating with biofilm development at 24, 48 and 72 h. Biofilm growth yield after 180 min incubation, when evaluated with the 200 mM glucose supplemented XTT, produced the most consistent readings on repetitive testing. It may be concluded that glucose supplementation of XTT could minimize variation and produce more accurate data for the XTT assay.
Persistent Identifierhttp://hdl.handle.net/10722/58158
ISSN
2015 SCImago Journal Rankings: 0.470
References

 

DC FieldValueLanguage
dc.contributor.authorda Silva, WJen_HK
dc.contributor.authorSeneviratne, Jen_HK
dc.contributor.authorParahitiyawa, Nen_HK
dc.contributor.authorRosa, EARen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authordel Bel Cury, AAen_HK
dc.date.accessioned2010-05-31T03:24:47Z-
dc.date.available2010-05-31T03:24:47Z-
dc.date.issued2008en_HK
dc.identifier.citationBrazilian Dental Journal, 2008, v. 19 n. 4, p. 364-369en_HK
dc.identifier.issn0103-6440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58158-
dc.description.abstract2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) reduction assay has been used to study Candida biofilm formation. However, considering that the XTT reduction assay is dependent on cell activity, its use for evaluating mature biofilms may lead to inaccuracies since biofilm bottom cell layers tend to be relatively quiescent at later stages of biofilm formation. The aim of this study was to improve XTT reduction assay by adding glucose supplements to the standard XTT formulation. Candida albicans ATCC 90028 was used to form 24-, 48- and 72-h biofilms. The oxidative activity at 90, 180 and 270 min of incubation was evaluated. The control consisted of standard XTT formulation without glucose supplements, and was modified by the addition of 50, 100 and 200 mM of glucose. The XTT assay with 200 mM glucose showed more accurate and consistent readings correlating with biofilm development at 24, 48 and 72 h. Biofilm growth yield after 180 min incubation, when evaluated with the 200 mM glucose supplemented XTT, produced the most consistent readings on repetitive testing. It may be concluded that glucose supplementation of XTT could minimize variation and produce more accurate data for the XTT assay.en_HK
dc.languageengen_HK
dc.publisherFundacao Odontologica de Ribeirao Preto. The Journal's web site is located at http://www.forp.usp.br/bdj/bdj.htmen_HK
dc.relation.ispartofBrazilian Dental Journalen_HK
dc.subjectBiofilmen_HK
dc.subjectCandida albicansen_HK
dc.subjectXTTen_HK
dc.titleImprovement of XTT assay performance for studies involving Candida albicans biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.emailSeneviratne, J:jaya@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySeneviratne, J=rp01372en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-63249106128en_HK
dc.identifier.hkuros154587en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63249106128&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue4en_HK
dc.identifier.spage364en_HK
dc.identifier.epage369en_HK
dc.publisher.placeBrazilen_HK
dc.identifier.scopusauthoridda Silva, WJ=23972574300en_HK
dc.identifier.scopusauthoridSeneviratne, J=6701897753en_HK
dc.identifier.scopusauthoridParahitiyawa, N=13408948800en_HK
dc.identifier.scopusauthoridRosa, EAR=7004952748en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoriddel Bel Cury, AA=35329490200en_HK

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