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Article: Proposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases

TitleProposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phases
Authors
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jmicmeth
Citation
Journal Of Microbiological Methods, 2009, v. 78 n. 2, p. 171-174 How to Cite?
AbstractBiofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties of adjacent tissues causing morphofunctional failure. Many studies have shown that the expression of virulence attributes is maximized when microbes form such communities. This study evaluated the differential phospholipasic activity of Candida albicans SC5314 grown in planktonic phase and in biofilm. We propose two distinct protocols for the colorimetric evaluation of phosphatidylcholine hydrolysis in neutral and acidic conditions. The results showed that both protocols are suitable for the proposed intention and that 72 h-old planktonic cultures of C. albicans SC5314 secrete higher quantities of neutral (6.42-fold) and acidic (3.85-fold) phospholipases than biofilms. © 2009 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58120
ISSN
2015 Impact Factor: 1.857
2015 SCImago Journal Rankings: 0.805
ISI Accession Number ID
Funding AgencyGrant Number
PUCPR
Funding Information:

This study was part of the activities of LT. and B.F.F. to receive their Advanced Diplomas in Microbiology. The study was carried out with intramural funds of PUCPR.

References

 

DC FieldValueLanguage
dc.contributor.authorTaniguchi, Len_HK
dc.contributor.authorde Fátima Faria, Ben_HK
dc.contributor.authorRosa, RTen_HK
dc.contributor.authorde Paula e Carvalho, Aen_HK
dc.contributor.authorGursky, LCen_HK
dc.contributor.authorElifioEsposito, SLen_HK
dc.contributor.authorParahitiyawa, Nen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorRosa, EARen_HK
dc.date.accessioned2010-05-31T03:24:08Z-
dc.date.available2010-05-31T03:24:08Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Microbiological Methods, 2009, v. 78 n. 2, p. 171-174en_HK
dc.identifier.issn0167-7012en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58120-
dc.description.abstractBiofilms are aggregates of microorganisms living in multilayered structures inside polymeric matrices onto surfaces. These biofilms may subvert the physiological properties of adjacent tissues causing morphofunctional failure. Many studies have shown that the expression of virulence attributes is maximized when microbes form such communities. This study evaluated the differential phospholipasic activity of Candida albicans SC5314 grown in planktonic phase and in biofilm. We propose two distinct protocols for the colorimetric evaluation of phosphatidylcholine hydrolysis in neutral and acidic conditions. The results showed that both protocols are suitable for the proposed intention and that 72 h-old planktonic cultures of C. albicans SC5314 secrete higher quantities of neutral (6.42-fold) and acidic (3.85-fold) phospholipases than biofilms. © 2009 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jmicmethen_HK
dc.relation.ispartofJournal of Microbiological Methodsen_HK
dc.rightsJournal of Microbiological Methods. Copyright © Elsevier BV.en_HK
dc.subject.meshBiofilmsen_HK
dc.subject.meshCandida albicans - enzymology - physiologyen_HK
dc.subject.meshColorimetry - economics - methodsen_HK
dc.subject.meshFungal Proteins - metabolismen_HK
dc.subject.meshPhosphatidylcholines - metabolismen_HK
dc.subject.meshPhospholipases - metabolismen_HK
dc.titleProposal of a low-cost protocol for colorimetric semi-quantification of secretory phospholipase by Candida albicans grown in planktonic and biofilm phasesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0167-7012&volume=78&issue=2&spage=171&epage=4&date=2009&atitle=Proposal+of+a+low-cost+protocol+for+colorimetric+semi-quantification+of+secretory+phospholipase+by+Candida+albicans+grown+in+planktonic+and+biofilm+phasesen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.mimet.2009.05.012en_HK
dc.identifier.pmid19464327-
dc.identifier.scopuseid_2-s2.0-67650429636en_HK
dc.identifier.hkuros155956en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67650429636&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume78en_HK
dc.identifier.issue2en_HK
dc.identifier.spage171en_HK
dc.identifier.epage174en_HK
dc.identifier.isiWOS:000268820700011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridTaniguchi, L=34971947200en_HK
dc.identifier.scopusauthoridde Fátima Faria, B=26635010100en_HK
dc.identifier.scopusauthoridRosa, RT=35576111200en_HK
dc.identifier.scopusauthoridde Paula e Carvalho, A=26634977100en_HK
dc.identifier.scopusauthoridGursky, LC=14827791100en_HK
dc.identifier.scopusauthoridElifioEsposito, SL=23008409600en_HK
dc.identifier.scopusauthoridParahitiyawa, N=13408948800en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridRosa, EAR=7004952748en_HK
dc.identifier.citeulike5261127-

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