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Article: Antifungal activity of black tea polyphenols (catechins and theaflavins) against candida species

TitleAntifungal activity of black tea polyphenols (catechins and theaflavins) against candida species
Authors
Issue Date2009
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/CHE
Citation
Chemotherapy, 2009, v. 55 n. 3, p. 189-196 How to Cite?
AbstractBackground/Aims: The polyphenols catechins and theaflavins in black tea have been shown to possess many medicinal properties, including anticancer activity and some antifungal characteristics, but there have been few studies of their anti-Candida activity. In this paper we report the results of our study of the anti-Candida activity of tea polyphenols. Methods: The effects of 4 different concentrations of catechins and theaflavins were evaluated on 5 isolates each of 5 Candida species employing an agar diffusion growth inhibition assay. The minimum inhibitory concentration (MIC) of the polyphenols against C. albicans was determined. The post-antifungal effect (PAFE) of the polyphenols for C. albicans was investigated. C. albicans cells exposed to polyphenols were studied using a scanning electron microscope (SEM). Results: Both polyphenols showed anti-Candida activity against all tested Candida species and demonstrated a MIC of 6.25 mg/ml for C. albicans. C. glabrata was found to be the most sensitive species followed by C. parapsilosis, C. albicans, C. krusei and C. tropicalis (p < 0.05 for all). Significant intraspecies variations in sensitivity were noted among C. parapsilosis and C. tropicalis (p < 0.001) for both polyphenols. Theaflavins displayed standard PAFE while catechins showed a paradoxical PAFE with all isolates of C. albicans. SEM revealed considerable cell wall damage of C. albicans cells exposed to the polyphenols. Conclusion: The study reveals for the first time the anti-Candida properties of black tea polyphenols that may find therapeutic applications in future. Copyright © 2009 S. Karger AG, Basel.
Persistent Identifierhttp://hdl.handle.net/10722/58093
ISSN
2015 Impact Factor: 0.992
2015 SCImago Journal Rankings: 0.630
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council
Committee on Research and Conference Grants of the Hong Kong SAR, China
Funding Information:

The authors wish to acknowledge the support received from the Research Grants Council and the Committee on Research and Conference Grants of the Hong Kong SAR, China, for the work described in this paper. The authors are also grateful to Dr. Jayampath Seneviratne for critically reading the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorSitheeque, MAMen_HK
dc.contributor.authorPanagoda, GJen_HK
dc.contributor.authorYau, Jen_HK
dc.contributor.authorAmarakoon, AMTen_HK
dc.contributor.authorUdagama, URNen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-05-31T03:23:41Z-
dc.date.available2010-05-31T03:23:41Z-
dc.date.issued2009en_HK
dc.identifier.citationChemotherapy, 2009, v. 55 n. 3, p. 189-196en_HK
dc.identifier.issn0009-3157en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58093-
dc.description.abstractBackground/Aims: The polyphenols catechins and theaflavins in black tea have been shown to possess many medicinal properties, including anticancer activity and some antifungal characteristics, but there have been few studies of their anti-Candida activity. In this paper we report the results of our study of the anti-Candida activity of tea polyphenols. Methods: The effects of 4 different concentrations of catechins and theaflavins were evaluated on 5 isolates each of 5 Candida species employing an agar diffusion growth inhibition assay. The minimum inhibitory concentration (MIC) of the polyphenols against C. albicans was determined. The post-antifungal effect (PAFE) of the polyphenols for C. albicans was investigated. C. albicans cells exposed to polyphenols were studied using a scanning electron microscope (SEM). Results: Both polyphenols showed anti-Candida activity against all tested Candida species and demonstrated a MIC of 6.25 mg/ml for C. albicans. C. glabrata was found to be the most sensitive species followed by C. parapsilosis, C. albicans, C. krusei and C. tropicalis (p < 0.05 for all). Significant intraspecies variations in sensitivity were noted among C. parapsilosis and C. tropicalis (p < 0.001) for both polyphenols. Theaflavins displayed standard PAFE while catechins showed a paradoxical PAFE with all isolates of C. albicans. SEM revealed considerable cell wall damage of C. albicans cells exposed to the polyphenols. Conclusion: The study reveals for the first time the anti-Candida properties of black tea polyphenols that may find therapeutic applications in future. Copyright © 2009 S. Karger AG, Basel.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/CHEen_HK
dc.relation.ispartofChemotherapyen_HK
dc.rightsChemotherapy. Copyright © S Karger AG.en_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshBiflavonoids - pharmacologyen_HK
dc.subject.meshCandida - drug effectsen_HK
dc.subject.meshCatechin - pharmacologyen_HK
dc.subject.meshMicrobial Sensitivity Testsen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshTea - chemistryen_HK
dc.titleAntifungal activity of black tea polyphenols (catechins and theaflavins) against candida speciesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0009-3157&volume=55&issue=3&spage=189&epage=196&date=2009&atitle=Antifungal+activity+of+black+tea+polyphenols+(catechins+and+theaflavins)+against+Candida+speciesen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000216836en_HK
dc.identifier.pmid19420933-
dc.identifier.scopuseid_2-s2.0-65449127456en_HK
dc.identifier.hkuros155513en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65449127456&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume55en_HK
dc.identifier.issue3en_HK
dc.identifier.spage189en_HK
dc.identifier.epage196en_HK
dc.identifier.isiWOS:000265863400008-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridSitheeque, MAM=6505934676en_HK
dc.identifier.scopusauthoridPanagoda, GJ=6507149433en_HK
dc.identifier.scopusauthoridYau, J=7102167568en_HK
dc.identifier.scopusauthoridAmarakoon, AMT=6505710368en_HK
dc.identifier.scopusauthoridUdagama, URN=26535326500en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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