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Article: Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans

TitlePhenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans
Authors
Issue Date2008
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 2008, v. 57 n. 10, p. 1277-1281 How to Cite?
AbstractThe current assumption that Candida albicans is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of C. albicans strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase (P > 0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment (P <0.0001). However, a consistent increment (P <0.0001) in Sap secretion was detected when cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of C. albicans. © 2008 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/58073
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID
Funding AgencyGrant Number
Araucaria Foundation9042
CAPES-Coordination for the Improvement of Higher Education PersonnelBEX 0059/06-7
OBU-PPDH-HKU
Funding Information:

The authors are indebted to Professor Jose F. Hofling (FOP-UNICAMP) and Dr Thein Zaw Moe (OBU-PPDH-HKU) for the yeast strains used in this study. This work was supported by grants supplied by the Araucaria Foundation (Process 9042) and CAPES-Coordination for the Improvement of Higher Education Personnel (Process BEX 0059/06-7) and intramural funds from OBU-PPDH-HKU.

References

 

DC FieldValueLanguage
dc.contributor.authorRosa, EARen_HK
dc.contributor.authorRached, RNen_HK
dc.contributor.authorIgnácio, SAen_HK
dc.contributor.authorRosa, RTen_HK
dc.contributor.authorDa Silva, WJen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-05-31T03:23:21Z-
dc.date.available2010-05-31T03:23:21Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Medical Microbiology, 2008, v. 57 n. 10, p. 1277-1281en_HK
dc.identifier.issn0022-2615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58073-
dc.description.abstractThe current assumption that Candida albicans is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of C. albicans strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase (P > 0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment (P <0.0001). However, a consistent increment (P <0.0001) in Sap secretion was detected when cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of C. albicans. © 2008 SGM.en_HK
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_HK
dc.relation.ispartofJournal of Medical Microbiologyen_HK
dc.subject.meshAnaerobiosis - physiologyen_HK
dc.subject.meshCandida albicans - metabolism - pathogenicityen_HK
dc.subject.meshFungal Proteins - genetics - metabolismen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Fungal - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPeriodontal Pocket - microbiologyen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshVirulenceen_HK
dc.titlePhenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicansen_HK
dc.typeArticleen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/jmm.0.2008/001107-0en_HK
dc.identifier.pmid18809558-
dc.identifier.scopuseid_2-s2.0-53749107829en_HK
dc.identifier.hkuros152489en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-53749107829&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume57en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1277en_HK
dc.identifier.epage1281en_HK
dc.identifier.isiWOS:000259950400015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRosa, EAR=7004952748en_HK
dc.identifier.scopusauthoridRached, RN=9534952500en_HK
dc.identifier.scopusauthoridIgnácio, SA=8408648400en_HK
dc.identifier.scopusauthoridRosa, RT=35576111200en_HK
dc.identifier.scopusauthoridDa Silva, WJ=23972574300en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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