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Article: Mesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering

TitleMesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering
Authors
Issue Date2010
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261
Citation
Tissue Engineering - Part C: Methods, 2010, v. 16 n. 2, p. 225-235 How to Cite?
AbstractThere is a demonstrated clinical need for alternatives of autologous fresh bone graft with excellent biological performance in osteoconductivity, osteoinductivity, and osteogenicity. We previously developed a collagen microencapsulation technology entrapping bone marrow-derived mesenchymal stem cells (MSCs) in a biomimetic collagen fiber meshwork and produced injectable collagen-MSC microspheres. In this study, we hypothesize that injectable microspheres with osteoconductivity, osteogenicity, and osteoinductivity can be fabricated by differentiating the encapsulated MSCs, from either human or mouse sources, toward osteogenic lineages in these three-dimensional microspheres. The osteogenicity, osteoconductivity, and osteoinductivity of the microspheres were evaluated in vitro. Osteogenic markers of the differentiating MSCs including alkaline phosphatase and calcium deposition showed positive staining. Osteoconductivity of the collagen meshwork in the microsphere was demonstrated by the presence of calcium phosphate deposits among the collagen fibers and by the significantly increased calcium content extracted from the microspheres. Moreover, osteoinductivity of the MSC-encapsulated microspheres was demonstrated by the ability to induce osteogenic differentiation of undifferentiated MSCs in both contact and noncontact coculture. This study contributes toward the future development of injectable alternatives for fresh bone grafts using autologous MSCs. © 2010, Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/58064
ISSN
2015 SCImago Journal Rankings: 1.141
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council and Innovation and Technology Commission Hong Kong GovernmentITS/133/08
University Research Committee10400308
Strategic Research Theme of the University of Hong Kong (Biomedical Engineering)
Funding Information:

This work was supported by grants from Research Grant Council and Innovation and Technology Commission (ITS/133/08) of the Hong Kong Government, and seed funding from University Research Committee (10400308) and Strategic Research Theme of the University of Hong Kong (Biomedical Engineering). The authors thank Dr. Y.Y. Li for assistance with the osteoinductivity assay and Mr. K.O. Lam and Ms. Sisu Mo for assistance with the bone marrow aspiration in mice.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorChan, BPen_HK
dc.contributor.authorHui, TYen_HK
dc.contributor.authorWong, MYen_HK
dc.contributor.authorYip, KHKen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2010-05-31T03:23:12Z-
dc.date.available2010-05-31T03:23:12Z-
dc.date.issued2010en_HK
dc.identifier.citationTissue Engineering - Part C: Methods, 2010, v. 16 n. 2, p. 225-235en_HK
dc.identifier.issn1937-3384en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58064-
dc.description.abstractThere is a demonstrated clinical need for alternatives of autologous fresh bone graft with excellent biological performance in osteoconductivity, osteoinductivity, and osteogenicity. We previously developed a collagen microencapsulation technology entrapping bone marrow-derived mesenchymal stem cells (MSCs) in a biomimetic collagen fiber meshwork and produced injectable collagen-MSC microspheres. In this study, we hypothesize that injectable microspheres with osteoconductivity, osteogenicity, and osteoinductivity can be fabricated by differentiating the encapsulated MSCs, from either human or mouse sources, toward osteogenic lineages in these three-dimensional microspheres. The osteogenicity, osteoconductivity, and osteoinductivity of the microspheres were evaluated in vitro. Osteogenic markers of the differentiating MSCs including alkaline phosphatase and calcium deposition showed positive staining. Osteoconductivity of the collagen meshwork in the microsphere was demonstrated by the presence of calcium phosphate deposits among the collagen fibers and by the significantly increased calcium content extracted from the microspheres. Moreover, osteoinductivity of the MSC-encapsulated microspheres was demonstrated by the ability to induce osteogenic differentiation of undifferentiated MSCs in both contact and noncontact coculture. This study contributes toward the future development of injectable alternatives for fresh bone grafts using autologous MSCs. © 2010, Mary Ann Liebert, Inc.en_HK
dc.languageengen_HK
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=261en_HK
dc.relation.ispartofTissue Engineering - Part C: Methodsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Tissue Engineering - Part C: Methods] © [2010] [copyright Mary Ann Liebert, Inc.]; [Tissue Engineering - Part C: Methods] is available online at: http://www.liebertonline.com.-
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Regeneration - physiologyen_HK
dc.subject.meshBone and Bones - physiologyen_HK
dc.subject.meshCell Culture Techniques - methodsen_HK
dc.subject.meshCell Differentiation - drug effects - physiologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCollagen - administration & dosage - chemistry - pharmacologyen_HK
dc.subject.meshDrug Compoundingen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMesenchymal Stem Cell Transplantation - instrumentation - methodsen_HK
dc.subject.meshMesenchymal Stem Cells - chemistry - cytology - physiologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshOsteogenesis - drug effects - physiologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshTissue Engineering - methodsen_HK
dc.titleMesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineeringen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_HK
dc.identifier.emailYip, KHK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.emailChan, GCF: gcfchan@hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.identifier.authorityYip, KHK=rp00027en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/ten.tec.2008.0709en_HK
dc.identifier.pmid20367213en_HK
dc.identifier.scopuseid_2-s2.0-77952374120en_HK
dc.identifier.hkuros164809en_HK
dc.identifier.hkuros163811-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77952374120&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue2en_HK
dc.identifier.spage225en_HK
dc.identifier.epage235en_HK
dc.identifier.isiWOS:000276263100006-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectCreating Stable Tissue Interfaces for Bioengineered Intervertebral Disc Segment-
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.scopusauthoridHui, TY=36192803300en_HK
dc.identifier.scopusauthoridWong, MY=36053841600en_HK
dc.identifier.scopusauthoridYip, KHK=25423244900en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK

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