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Article: Effect of filamentation and mode of growth on antifungal susceptibility of Candida albicans

TitleEffect of filamentation and mode of growth on antifungal susceptibility of Candida albicans
Authors
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijantimicag
Citation
International Journal Of Antimicrobial Agents, 2009, v. 34 n. 4, p. 333-339 How to Cite?
AbstractBiofilm formation involving profuse hyphal growth is a major characteristic of Candida spp. and confers higher antifungal resistance than its planktonic mode of growth. We investigated the antifungal susceptibility of Candida albicans and its hyphal mutants (Δefg1/efg1, Δcph1/cph1 and ΔΔcph1/cph1 efg1/efg1) to commonly used antifungals during planktonic, adhesion and biofilm modes of growth. The minimum inhibitory concentration (MIC) of each antifungal agent was determined for a lower inoculum (1 × 10 3 cells/mL) and higher inoculum (1 × 10 7 cells/mL) of planktonic Candida. Furthermore, MICs of C. albicans biofilms and adhesion modes of growth were determined with a standard XTT assay. Candida albicans in adhesion and biofilm modes of growth, but not in planktonic mode, were resistant to all five antifungal agents tested. Although Δefg1/efg1 and ΔΔcph1/cph1 efg1/efg1 mutants formed less biofilm than wild-type C. albicans SC5314, they were similarly resistant to caspofungin. However, these mutants were more sensitive to amphotericin B and nystatin than the wild-type. Adhesion per se confers increased resistance to antifungal agents, which is further pronounced in the biofilm mode of Candida. Filamentation does not appear to be a major determinant of the antifungal resistance in Candida biofilms. © 2009 Elsevier B.V. and the International Society of Chemotherapy.
Persistent Identifierhttp://hdl.handle.net/10722/58033
ISSN
2015 Impact Factor: 4.097
2015 SCImago Journal Rankings: 1.630
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7624/06M
Funding Information:

This work was supported by the Hong Kong Research Grants Council (RGC No. HKU 7624/06M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorWatamoto, Ten_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorJayatilake, JAMSen_HK
dc.contributor.authorEgusa, Hen_HK
dc.contributor.authorYatani, Hen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.date.accessioned2010-05-31T03:22:41Z-
dc.date.available2010-05-31T03:22:41Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Antimicrobial Agents, 2009, v. 34 n. 4, p. 333-339en_HK
dc.identifier.issn0924-8579en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58033-
dc.description.abstractBiofilm formation involving profuse hyphal growth is a major characteristic of Candida spp. and confers higher antifungal resistance than its planktonic mode of growth. We investigated the antifungal susceptibility of Candida albicans and its hyphal mutants (Δefg1/efg1, Δcph1/cph1 and ΔΔcph1/cph1 efg1/efg1) to commonly used antifungals during planktonic, adhesion and biofilm modes of growth. The minimum inhibitory concentration (MIC) of each antifungal agent was determined for a lower inoculum (1 × 10 3 cells/mL) and higher inoculum (1 × 10 7 cells/mL) of planktonic Candida. Furthermore, MICs of C. albicans biofilms and adhesion modes of growth were determined with a standard XTT assay. Candida albicans in adhesion and biofilm modes of growth, but not in planktonic mode, were resistant to all five antifungal agents tested. Although Δefg1/efg1 and ΔΔcph1/cph1 efg1/efg1 mutants formed less biofilm than wild-type C. albicans SC5314, they were similarly resistant to caspofungin. However, these mutants were more sensitive to amphotericin B and nystatin than the wild-type. Adhesion per se confers increased resistance to antifungal agents, which is further pronounced in the biofilm mode of Candida. Filamentation does not appear to be a major determinant of the antifungal resistance in Candida biofilms. © 2009 Elsevier B.V. and the International Society of Chemotherapy.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ijantimicagen_HK
dc.relation.ispartofInternational Journal of Antimicrobial Agentsen_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshBiofilms - drug effects - growth & developmenten_HK
dc.subject.meshCandida albicans - drug effects - genetics - growth & developmenten_HK
dc.subject.meshCell Adhesionen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshDrug Resistance, Fungalen_HK
dc.subject.meshFungal Proteins - genetics - metabolismen_HK
dc.subject.meshGene Expression Regulation, Fungalen_HK
dc.subject.meshHyphae - genetics - growth & developmenten_HK
dc.subject.meshMicrobial Sensitivity Testsen_HK
dc.subject.meshMutationen_HK
dc.subject.meshPlankton - drug effects - growth & developmenten_HK
dc.titleEffect of filamentation and mode of growth on antifungal susceptibility of Candida albicansen_HK
dc.typeArticleen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ijantimicag.2009.03.008en_HK
dc.identifier.pmid19376687-
dc.identifier.scopuseid_2-s2.0-67651229521en_HK
dc.identifier.hkuros155468en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67651229521&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue4en_HK
dc.identifier.spage333en_HK
dc.identifier.epage339en_HK
dc.identifier.isiWOS:000269275100008-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectCandida biofilms: molecular mechanisms and clinical implications-
dc.identifier.scopusauthoridWatamoto, T=24171949100en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_HK
dc.identifier.scopusauthoridEgusa, H=6602170721en_HK
dc.identifier.scopusauthoridYatani, H=7004428080en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.citeulike5468408-

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