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Article: Effects of lead exposure on alpha-synuclein and p53 transcription

TitleEffects of lead exposure on alpha-synuclein and p53 transcription
Authors
KeywordsAlpha-synuclein
p53
Real-time PCR
ATDC5
Aging
Cancer
Issue Date2009
PublisherScientific Research Publishing
Citation
Journal of Biomedical Science and Engineering, 2009, v. 2 n. 2, p. 86-89 How to Cite?
AbstractObjective: Epidemiological studies have found that lead exposure increases the risk for Park-inson’s disease and patients with Parkinson’s disease have lower odds of developing non-smoking-related cancer (1). It would be inter-esting therefore to find the molecular links be-tween Parkinson’s disease and cancer. To do this, we studied mRNA expression of alpha-synuclein gene, a promising genetic marker for Parkinson’s disease, and expression of the tu-mor suppressor gene p53 after oxidative stress induced by lead. Methods: We used ATDC5 cell line as a model of tumor and treated by lead nitrate for 0, 2, 4, 16, 24 and 48 hours. The mRNAs of alpha-synuclein and p53 were quan-tified by reverse transcriptase polymerase chain reaction and expressed as mean (±SD) for 3 samples at each time point. Results: Ex-pression of both of alpha-synuclein and p53 mRNA increased with increasing exposure of lead treatment. The levels of alpha-synuclein and p53 mRNA were correlated with each other (r=0.9830; P<0.001). Conclusion: We propose that lead’s neurotoxicity in PD is caused by al-pha-synuclein expression and aggregation, which releases the inhibitory influence of al-pha-synuclein on p53 expression, thereby al-lowing p53 to act as the cell’s guardian of the genome and reduce tumorigenic potential. Treatments that reduce alpha-synuclein aggre-gation may need to account for a concomitant reduction in p53’s protective effect.
Persistent Identifierhttp://hdl.handle.net/10722/58024
ISSN

 

DC FieldValueLanguage
dc.contributor.authorZuo, Pen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-05-31T03:22:32Z-
dc.date.available2010-05-31T03:22:32Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal of Biomedical Science and Engineering, 2009, v. 2 n. 2, p. 86-89en_HK
dc.identifier.issn1937-6871en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58024-
dc.description.abstractObjective: Epidemiological studies have found that lead exposure increases the risk for Park-inson’s disease and patients with Parkinson’s disease have lower odds of developing non-smoking-related cancer (1). It would be inter-esting therefore to find the molecular links be-tween Parkinson’s disease and cancer. To do this, we studied mRNA expression of alpha-synuclein gene, a promising genetic marker for Parkinson’s disease, and expression of the tu-mor suppressor gene p53 after oxidative stress induced by lead. Methods: We used ATDC5 cell line as a model of tumor and treated by lead nitrate for 0, 2, 4, 16, 24 and 48 hours. The mRNAs of alpha-synuclein and p53 were quan-tified by reverse transcriptase polymerase chain reaction and expressed as mean (±SD) for 3 samples at each time point. Results: Ex-pression of both of alpha-synuclein and p53 mRNA increased with increasing exposure of lead treatment. The levels of alpha-synuclein and p53 mRNA were correlated with each other (r=0.9830; P<0.001). Conclusion: We propose that lead’s neurotoxicity in PD is caused by al-pha-synuclein expression and aggregation, which releases the inhibitory influence of al-pha-synuclein on p53 expression, thereby al-lowing p53 to act as the cell’s guardian of the genome and reduce tumorigenic potential. Treatments that reduce alpha-synuclein aggre-gation may need to account for a concomitant reduction in p53’s protective effect.-
dc.languageengen_HK
dc.publisherScientific Research Publishingen_HK
dc.relation.ispartofJournal of Biomedical Science and Engineeringen_HK
dc.subjectAlpha-synuclein-
dc.subjectp53-
dc.subjectReal-time PCR-
dc.subjectATDC5-
dc.subjectAging-
dc.subjectCancer-
dc.titleEffects of lead exposure on alpha-synuclein and p53 transcriptionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-6871&volume=2&spage=86&epage=89&date=2009&atitle=Effects+of+lead+exposure+on+alpha-synuclein+and+p53+transcriptionen_HK
dc.identifier.emailZuo, P: peijunzuo@lycos.comen_HK
dc.identifier.emailRabie, ABM: rabie@hkusua.hku.hken_HK
dc.identifier.authorityZuo, P=rp00050en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.4236/jbise.2009.22016-
dc.identifier.hkuros155834en_HK

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