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Article: Effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the viability, proliferation and differentiation of osteoblast-like cells cultured on a chemically modified titanium surface

TitleEffect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the viability, proliferation and differentiation of osteoblast-like cells cultured on a chemically modified titanium surface
Authors
Issue Date2009
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLR
Citation
Clinical Oral Implants Research, 2009, v. 20 n. 5, p. 452-457 How to Cite?
AbstractAim: The aim of the present study was to assess the influence of the chemical characteristics and roughness of titanium surfaces on the viability, proliferation and differentiation of osteoblast-like cells cultured in a medium supplemented with recombinant human bone morphogenetic protein-7 (rhBMP-7). Material and methods: Osteo-1 cells were grown on titanium disks presenting with the following surfaces: (1) machined, (2) coarse grit-blasted and acid-attacked (SLA) and (3) chemically modified SLA (SLAmod) in the absence or presence of 20 ng/ml rhBMP-7 in culture medium. The viability and number of osteo-1 cells were evaluated after 24 h. Analyses of total protein content (TP) and alkaline phosphatase (AP) activity at 7, 14 and 21 days, collagen content at 7 and 21 days and mineralized matrix formation at 21 days were performed. Results: Cell viability (P=0.5516), cell number (P=0.3485), collagen content (P=0.1165) and mineralized matrix formation (P=0.5319) were not affected by the different surface configurations or by the addition of rhBMP-7 to the medium. Osteo-1 cells cultured on SLA surfaces showed a significant increase in TP at 21 days. The ALPase/TP ratio (P=0.00001) was affected by treatment and time. Conclusion: The results suggest that the addition of rhBMP-7 to the culture medium did not exert any effect on the viability, proliferation or differentiation of osteoblast-like cells grown on the different surfaces tested. All titanium surfaces analyzed allowed the complete expression of the osteoblast phenotype such as matrix mineralization by osteo-1 cells. © 2009 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/58023
ISSN
2015 Impact Factor: 3.464
2015 SCImago Journal Rankings: 1.427
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTogashi, AYen_HK
dc.contributor.authorCirano, FRen_HK
dc.contributor.authorMarques, MMen_HK
dc.contributor.authorPustiglioni, FEen_HK
dc.contributor.authorLang, NPen_HK
dc.contributor.authorLima, LAPAen_HK
dc.date.accessioned2010-05-31T03:22:31Z-
dc.date.available2010-05-31T03:22:31Z-
dc.date.issued2009en_HK
dc.identifier.citationClinical Oral Implants Research, 2009, v. 20 n. 5, p. 452-457en_HK
dc.identifier.issn0905-7161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58023-
dc.description.abstractAim: The aim of the present study was to assess the influence of the chemical characteristics and roughness of titanium surfaces on the viability, proliferation and differentiation of osteoblast-like cells cultured in a medium supplemented with recombinant human bone morphogenetic protein-7 (rhBMP-7). Material and methods: Osteo-1 cells were grown on titanium disks presenting with the following surfaces: (1) machined, (2) coarse grit-blasted and acid-attacked (SLA) and (3) chemically modified SLA (SLAmod) in the absence or presence of 20 ng/ml rhBMP-7 in culture medium. The viability and number of osteo-1 cells were evaluated after 24 h. Analyses of total protein content (TP) and alkaline phosphatase (AP) activity at 7, 14 and 21 days, collagen content at 7 and 21 days and mineralized matrix formation at 21 days were performed. Results: Cell viability (P=0.5516), cell number (P=0.3485), collagen content (P=0.1165) and mineralized matrix formation (P=0.5319) were not affected by the different surface configurations or by the addition of rhBMP-7 to the medium. Osteo-1 cells cultured on SLA surfaces showed a significant increase in TP at 21 days. The ALPase/TP ratio (P=0.00001) was affected by treatment and time. Conclusion: The results suggest that the addition of rhBMP-7 to the culture medium did not exert any effect on the viability, proliferation or differentiation of osteoblast-like cells grown on the different surfaces tested. All titanium surfaces analyzed allowed the complete expression of the osteoblast phenotype such as matrix mineralization by osteo-1 cells. © 2009 John Wiley & Sons A/S.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CLRen_HK
dc.relation.ispartofClinical Oral Implants Researchen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBiocompatible Materials - chemistry - pharmacologyen_HK
dc.subject.meshBone Morphogenetic Protein 7 - physiologyen_HK
dc.subject.meshCalcification, Physiologic - drug effects - physiologyen_HK
dc.subject.meshCell Differentiation - drug effects - physiologyen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCell Survival - drug effects - physiologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshHumansen_HK
dc.subject.meshOsteoblasts - cytology - drug effects - physiologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Wistaren_HK
dc.subject.meshRecombinant Proteinsen_HK
dc.subject.meshSurface Propertiesen_HK
dc.subject.meshTitanium - chemistry - pharmacologyen_HK
dc.titleEffect of recombinant human bone morphogenetic protein-7 (rhBMP-7) on the viability, proliferation and differentiation of osteoblast-like cells cultured on a chemically modified titanium surfaceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0905-7161&volume=20&spage=452&epage=457&date=2009&atitle=Effect+of+recombinant+human+bone+morphogenetic+protein-7+(rhBMP-7)+on+the+viability,+proliferation+and+differentiation+of+osteoblast-like+cells+cultured+on+a+chemically+modified+titanium+surfaceen_HK
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_HK
dc.identifier.authorityLang, NP=rp00031en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0501.2008.01669.xen_HK
dc.identifier.pmid19250243-
dc.identifier.scopuseid_2-s2.0-64649095888en_HK
dc.identifier.hkuros165441en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64649095888&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue5en_HK
dc.identifier.spage452en_HK
dc.identifier.epage457en_HK
dc.identifier.isiWOS:000265145700003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTogashi, AY=23013169100en_HK
dc.identifier.scopusauthoridCirano, FR=23011772300en_HK
dc.identifier.scopusauthoridMarques, MM=7201744115en_HK
dc.identifier.scopusauthoridPustiglioni, FE=6602339267en_HK
dc.identifier.scopusauthoridLang, NP=7201577367en_HK
dc.identifier.scopusauthoridLima, LAPA=7102364941en_HK
dc.identifier.citeulike4325690-

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