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Article: Biomarker discovery in clinical proteomics: Strategies for exposing low abundant proteins

TitleBiomarker discovery in clinical proteomics: Strategies for exposing low abundant proteins
Authors
KeywordsBiomarker discovery
Low abundant protein
Mass spectrometry
Proteome
Sample prefractionation
Two-dimensional gel electrophoresis
Issue Date2008
PublisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/cp/index.htm
Citation
Current Proteomics, 2008, v. 5 n. 2, p. 104-114 How to Cite?
AbstractDisease specific proteins are highly valuable as clinical biomarkers, which can be used in early diagnosis, monitoring disease progressions and evaluating therapies. The identification and characterization of protein biomarkers in different physiological and pathological sources of interest under diverse milieu represent a key area of clinical proteomics. However, owing to the inherent complexities and the large dynamic ranges of proteins, there are many practical issues and challenges in discovering the low-abundance, disease-specific biomarkers from heterogeneous tissues and biofluids. Thus, an integrated approach for selective protein pre-fractionation, purification and separation, is necessary to detect low-abundant biomarkers in proteomics research. This review will summarize recent advancements in clinical sample preparation for removing high abundant proteins, as well as the improved two-dimensional gel electrophoresis- and mass spectrometry-based technologies for resolving low-abundant proteins. We will also elaborate the proteomic strategies for targeting protein sub-populations with special interests, such as high molecular weight protein complexes, membrane or its associated proteins, and organelle specific proteins etc. We will emphasize the use of these strategies to interrogate the current proteomic researches in clinical biomarker discovery. ©2008 Bentham Science Publishers Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/58019
ISSN
2015 Impact Factor: 0.59
2015 SCImago Journal Rankings: 0.207
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Yen_HK
dc.contributor.authorSeneviratne, Jen_HK
dc.date.accessioned2010-05-31T03:22:27Z-
dc.date.available2010-05-31T03:22:27Z-
dc.date.issued2008en_HK
dc.identifier.citationCurrent Proteomics, 2008, v. 5 n. 2, p. 104-114en_HK
dc.identifier.issn1570-1646en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58019-
dc.description.abstractDisease specific proteins are highly valuable as clinical biomarkers, which can be used in early diagnosis, monitoring disease progressions and evaluating therapies. The identification and characterization of protein biomarkers in different physiological and pathological sources of interest under diverse milieu represent a key area of clinical proteomics. However, owing to the inherent complexities and the large dynamic ranges of proteins, there are many practical issues and challenges in discovering the low-abundance, disease-specific biomarkers from heterogeneous tissues and biofluids. Thus, an integrated approach for selective protein pre-fractionation, purification and separation, is necessary to detect low-abundant biomarkers in proteomics research. This review will summarize recent advancements in clinical sample preparation for removing high abundant proteins, as well as the improved two-dimensional gel electrophoresis- and mass spectrometry-based technologies for resolving low-abundant proteins. We will also elaborate the proteomic strategies for targeting protein sub-populations with special interests, such as high molecular weight protein complexes, membrane or its associated proteins, and organelle specific proteins etc. We will emphasize the use of these strategies to interrogate the current proteomic researches in clinical biomarker discovery. ©2008 Bentham Science Publishers Ltd.en_HK
dc.languageengen_HK
dc.publisherBentham Science Publishers Ltd. The Journal's web site is located at http://www.bentham.org/cp/index.htmen_HK
dc.relation.ispartofCurrent Proteomicsen_HK
dc.subjectBiomarker discoveryen_HK
dc.subjectLow abundant proteinen_HK
dc.subjectMass spectrometryen_HK
dc.subjectProteomeen_HK
dc.subjectSample prefractionationen_HK
dc.subjectTwo-dimensional gel electrophoresisen_HK
dc.titleBiomarker discovery in clinical proteomics: Strategies for exposing low abundant proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1570-1646&volume=5&spage=&epage=&date=2008&atitle=Biomarker+discovery+in+clinical+proteomics:+strategies+for+exposing+low+abundant+proteins.en_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailSeneviratne, J: jaya@hku.hken_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authoritySeneviratne, J=rp01372en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2174/157016408784911927en_HK
dc.identifier.scopuseid_2-s2.0-47249112230en_HK
dc.identifier.hkuros146089en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47249112230&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue2en_HK
dc.identifier.spage104en_HK
dc.identifier.epage114en_HK
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridSeneviratne, J=6701897753en_HK
dc.identifier.citeulike2974474-

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