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Article: Overexpression of Arabidopsis acyl-CoA binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence

TitleOverexpression of Arabidopsis acyl-CoA binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescence
Authors
KeywordsBiology - botany
Biology - biochemistry
Issue Date2010
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantcell.org
Citation
Plant Cell, 2010, v. 22 n. 5, p. 1463-1482 How to Cite?
AbstractIn Arabidopsis thaliana, a family of six genes (ACBP1 to ACBP6) encodes acyl-CoA binding proteins (ACBPs). Investigations on ACBP3 reported here show its upregulation upon dark treatment and in senescing rosettes. Transgenic Arabidopsis overexpressing ACBP3 (ACBP3-OEs) displayed accelerated leaf senescence, whereas an acbp3 T-DNA insertional mutant and ACBP3 RNA interference transgenic Arabidopsis lines were delayed in dark-induced leaf senescence. Acyl-CoA and lipid profiling revealed that the overexpression of ACBP3 led to an increase in acyl-CoA and phosphatidylethanolamine (PE) levels, whereas ACBP3 downregulation reduced PE content. Moreover, significant losses in phosphatidylcholine (PC) and phosphatidylinositol, and gains in phosphatidic acid (PA), lysophospholipids, and oxylipin-containing galactolipids (arabidopsides) were evident in 3-week-old dark-treated and 6-week-old premature senescing ACBP3-OEs. Such accu-mulation of PA and arabidopsides (A, B, D, E, and G) resulting from lipid peroxidation in ACBP3-OEs likely promoted leaf senescence. The N-terminal signal sequence/transmembrane domain in ACBP3 was shown to be essential in ACBP3-green fluorescent protein targeting and in promoting senescence. Observations that recombinant ACBP3 binds PC, PE, and unsaturated acyl-CoAs in vitro and that ACBP3 overexpression enhances degradation of the autophagy (ATG)-related protein ATG8 and disrupts autophagosome formation suggest a role for ACBP3 as a phospholipid binding protein involved in the regulation of leaf senescence by modulating membrane phospholipid metabolism and ATG8 stability in Arabidopsis. Accelerated senescence in ACBP3-OEs is dependent on salicylic acid but not jasmonic acid signaling. © 2010 American Society of Plant Biologists.
Persistent Identifierhttp://hdl.handle.net/10722/57626
ISSN
2014 Impact Factor: 9.338
2014 SCImago Journal Rankings: 4.832
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, ChinaHKU7047/07M
University of Hong Kong10208034
National Science FoundationEPS 0236913
MCB 0455318
DBI 0521587
Kansas Technology Enterprise Corporation
Kansas IDeA Network of Biomedical Research Excellence (INBRE) of the National Institutes of HealthP20RR16475
Kansas State University
Funding Information:

We thank M. Roth (Kansas Lipidomics Research Center) for lipid profiling, the ABRC for provision of npr1-5 and acbp3 mutant seeds, D. X. Xie (Tsinghua University) for the coi1-2 mutant, and S. F. Chen (University of Hong Kong) for provision of HPLC. This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (Project HKU7047/07M), and the University of Hong Kong (Project 10208034, postdoctoral fellowship to S.X. and studentships to W.G., Q.-F.C., S.-X.Z., S.-W.C., and J.M.). Lipid profiling was performed at the Kansas Lipidomics Research Center, where method development and instrument acquisition were supported by the National Science Foundation (EPS 0236913, MCB 0455318, and DBI 0521587), the Kansas Technology Enterprise Corporation, the Kansas IDeA Network of Biomedical Research Excellence (INBRE) of the National Institutes of Health (P20RR16475), and Kansas State University.

References

 

DC FieldValueLanguage
dc.contributor.authorXiao, Sen_HK
dc.contributor.authorGao, Wen_HK
dc.contributor.authorChen, QFen_HK
dc.contributor.authorChan, SWen_HK
dc.contributor.authorZheng, SXen_HK
dc.contributor.authorMa, Jen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorWelti, Ren_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-05-07T04:03:57Z-
dc.date.available2010-05-07T04:03:57Z-
dc.date.issued2010en_HK
dc.identifier.citationPlant Cell, 2010, v. 22 n. 5, p. 1463-1482en_HK
dc.identifier.issn1040-4651en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57626-
dc.description.abstractIn Arabidopsis thaliana, a family of six genes (ACBP1 to ACBP6) encodes acyl-CoA binding proteins (ACBPs). Investigations on ACBP3 reported here show its upregulation upon dark treatment and in senescing rosettes. Transgenic Arabidopsis overexpressing ACBP3 (ACBP3-OEs) displayed accelerated leaf senescence, whereas an acbp3 T-DNA insertional mutant and ACBP3 RNA interference transgenic Arabidopsis lines were delayed in dark-induced leaf senescence. Acyl-CoA and lipid profiling revealed that the overexpression of ACBP3 led to an increase in acyl-CoA and phosphatidylethanolamine (PE) levels, whereas ACBP3 downregulation reduced PE content. Moreover, significant losses in phosphatidylcholine (PC) and phosphatidylinositol, and gains in phosphatidic acid (PA), lysophospholipids, and oxylipin-containing galactolipids (arabidopsides) were evident in 3-week-old dark-treated and 6-week-old premature senescing ACBP3-OEs. Such accu-mulation of PA and arabidopsides (A, B, D, E, and G) resulting from lipid peroxidation in ACBP3-OEs likely promoted leaf senescence. The N-terminal signal sequence/transmembrane domain in ACBP3 was shown to be essential in ACBP3-green fluorescent protein targeting and in promoting senescence. Observations that recombinant ACBP3 binds PC, PE, and unsaturated acyl-CoAs in vitro and that ACBP3 overexpression enhances degradation of the autophagy (ATG)-related protein ATG8 and disrupts autophagosome formation suggest a role for ACBP3 as a phospholipid binding protein involved in the regulation of leaf senescence by modulating membrane phospholipid metabolism and ATG8 stability in Arabidopsis. Accelerated senescence in ACBP3-OEs is dependent on salicylic acid but not jasmonic acid signaling. © 2010 American Society of Plant Biologists.en_HK
dc.language.isoengen_HK
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantcell.orgen_HK
dc.relation.ispartofPlant Cellen_HK
dc.rightsThe Plant Cell. Copyright © American Society of Plant Biologists-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBiology - botanyen_HK
dc.subjectBiology - biochemistryen_HK
dc.titleOverexpression of Arabidopsis acyl-CoA binding protein ACBP3 promotes starvation-induced and age-dependent leaf senescenceen_HK
dc.typeArticleen_HK
dc.identifier.emailXiao, S: xiaoshi@graduate.hku.hken_HK
dc.identifier.emailWang, M: mfwang@hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityXiao, S=rp00817en_HK
dc.identifier.authorityWang, M=rp00800en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1105/tpc.110.075333en_HK
dc.identifier.pmid20442372-
dc.identifier.pmcidPMC2899868-
dc.identifier.scopuseid_2-s2.0-77954392870en_HK
dc.identifier.hkuros171737-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77954392870&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1463en_HK
dc.identifier.epage1482en_HK
dc.identifier.eissn1532-298X-
dc.identifier.isiWOS:000279253200007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXiao, S=7402022635en_HK
dc.identifier.scopusauthoridGao, W=36045713300en_HK
dc.identifier.scopusauthoridChen, QF=7406335399en_HK
dc.identifier.scopusauthoridChan, SW=24758152300en_HK
dc.identifier.scopusauthoridZheng, SX=36574604500en_HK
dc.identifier.scopusauthoridMa, J=9248720900en_HK
dc.identifier.scopusauthoridWang, M=7406691844en_HK
dc.identifier.scopusauthoridWelti, R=7004019768en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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