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Article: Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation

TitleFull factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
Authors
Issue Date2009
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2009, v. 4 n. 5 How to Cite?
AbstractAmongst all the internal gene segments (PB2. PB1, PA, NP, M and NS), the avian PB1 segment is the only one which was reassorted into the human H2N2 and H3N2 pandemic strains. This suggests that the reassortment of polymerase subunit genes between mammalian and avian influenza viruses might play roles for interspecies transmission. To test this hypothesis, we tested the compatibility between PB2, PB1, PA and NP derived from a H5N1 virus and a mammalian H1N1 virus. All 16 possible combinations of avian-mammalian chimeric viral ribonucleoproteins (vRNPs) were characterized. We showed that recombinant vRNPs with a mammalian PB2 and an avian PB1 had the strongest polymerase activities in human cells at all studied temperature. In addition, viruses with this specific PB2-PB1 combination could grow efficiently in cell cultures, especially at a high incubation temperature. These viruses were potent inducers of proinflammatory cytokines and chemokines in primary human macrophages and pneumocytes. Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure. These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern. © 2009 Li et al.
Persistent Identifierhttp://hdl.handle.net/10722/57597
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, OTWen_HK
dc.contributor.authorChan, MCWen_HK
dc.contributor.authorLeung, CSWen_HK
dc.contributor.authorChan, RWYen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorPoon, LLMen_HK
dc.date.accessioned2010-04-20T07:59:25Z-
dc.date.available2010-04-20T07:59:25Z-
dc.date.issued2009en_HK
dc.identifier.citationPlos One, 2009, v. 4 n. 5en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57597-
dc.description.abstractAmongst all the internal gene segments (PB2. PB1, PA, NP, M and NS), the avian PB1 segment is the only one which was reassorted into the human H2N2 and H3N2 pandemic strains. This suggests that the reassortment of polymerase subunit genes between mammalian and avian influenza viruses might play roles for interspecies transmission. To test this hypothesis, we tested the compatibility between PB2, PB1, PA and NP derived from a H5N1 virus and a mammalian H1N1 virus. All 16 possible combinations of avian-mammalian chimeric viral ribonucleoproteins (vRNPs) were characterized. We showed that recombinant vRNPs with a mammalian PB2 and an avian PB1 had the strongest polymerase activities in human cells at all studied temperature. In addition, viruses with this specific PB2-PB1 combination could grow efficiently in cell cultures, especially at a high incubation temperature. These viruses were potent inducers of proinflammatory cytokines and chemokines in primary human macrophages and pneumocytes. Viruses with this specific PB2-PB1 combination were also found to be more capable to generate adaptive mutations under a new selection pressure. These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern. © 2009 Li et al.en_HK
dc.language.isoengen_HK
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsPLoS ONE. Copyright © Public Library of Science.en_HK
dc.subject.meshAdaptation, Biologicalen_HK
dc.subject.meshCytokines - metabolismen_HK
dc.subject.meshInfluenza A virus - enzymology - geneticsen_HK
dc.subject.meshInfluenza in Birds - enzymologyen_HK
dc.subject.meshMammals - virologyen_HK
dc.titleFull factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptationen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, MCW: mchan@hku.hken_HK
dc.identifier.emailChan, RWY: reneewy@hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityChan, MCW=rp00420en_HK
dc.identifier.authorityChan, RWY=rp01596en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1371/journal.pone.0005658en_HK
dc.identifier.pmid19462010-
dc.identifier.pmcidPMC2680953en_HK
dc.identifier.scopuseid_2-s2.0-66249092809en_HK
dc.identifier.hkuros155999en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66249092809&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume4en_HK
dc.identifier.issue5en_HK
dc.identifier.isiWOS:000266331000016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, OTW=16230718400en_HK
dc.identifier.scopusauthoridChan, MCW=26654715500en_HK
dc.identifier.scopusauthoridLeung, CSW=7402612654en_HK
dc.identifier.scopusauthoridChan, RWY=26661379100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.citeulike4620545-

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