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Article: Identification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis

TitleIdentification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis
Authors
KeywordsChemistry
Analytical chemistry pharmacy and pharmacology
Issue Date2008
PublisherHindawi Publishing Corp. The Journal's web site is located at http://www.hindawi.com/journals/mbd/
Citation
Metal-Based Drugs, 2008, v. 2008 Article ID 289490 How to Cite?
AbstractHelicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/57290
ISSN
2014 SCImago Journal Rankings: 0.180
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Xen_HK
dc.contributor.authorGe, Ren_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorHe, QYen_HK
dc.date.accessioned2010-04-12T01:32:05Z-
dc.date.available2010-04-12T01:32:05Z-
dc.date.issued2008en_HK
dc.identifier.citationMetal-Based Drugs, 2008, v. 2008 Article ID 289490en_HK
dc.identifier.issn0793-0291en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57290-
dc.description.abstractHelicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed.en_HK
dc.languageengen_HK
dc.publisherHindawi Publishing Corp. The Journal's web site is located at http://www.hindawi.com/journals/mbd/en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectChemistryen_HK
dc.subjectAnalytical chemistry pharmacy and pharmacologyen_HK
dc.titleIdentification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0793-0291&volume=2008 Article ID 289490&spage=&epage=&date=2008&atitle=Identification+of+proteins+related+to+nickel+homeostasis+in+Helicobater+pylori+by+immobilized+metal+affinity+chromatography+and+two-dimensional+gel+electrophoresisen_HK
dc.identifier.emailGe, R: geruig@hkusua.hku.hken_HK
dc.identifier.emailChiu, JF: jfchiu@hkucc.hku.hken_HK
dc.identifier.emailSun, H: sunhui116@hotmail.comen_HK
dc.identifier.emailHe, QY: qyhe@hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1155/2008/289490en_HK
dc.identifier.pmid18288244en_HK
dc.identifier.pmcidPMC2225478en_HK
dc.identifier.hkuros141636-

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