File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1155/2008/289490
- PMID: 18288244
- Find via
Supplementary
-
Citations:
- PubMed Central: 0
- Appears in Collections:
Article: Identification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis
Title | Identification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis |
---|---|
Authors | |
Keywords | Chemistry Analytical chemistry pharmacy and pharmacology |
Issue Date | 2008 |
Publisher | Hindawi Publishing Corp. The Journal's web site is located at http://www.hindawi.com/journals/mbd/ |
Citation | Metal-Based Drugs, 2008, v. 2008 Article ID 289490 How to Cite? |
Abstract | Helicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed. |
Persistent Identifier | http://hdl.handle.net/10722/57290 |
ISSN | |
PubMed Central ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun, X | en_HK |
dc.contributor.author | Ge, R | en_HK |
dc.contributor.author | Chiu, JF | en_HK |
dc.contributor.author | Sun, H | en_HK |
dc.contributor.author | He, QY | en_HK |
dc.date.accessioned | 2010-04-12T01:32:05Z | - |
dc.date.available | 2010-04-12T01:32:05Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Metal-Based Drugs, 2008, v. 2008 Article ID 289490 | en_HK |
dc.identifier.issn | 0793-0291 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/57290 | - |
dc.description.abstract | Helicobacter pylori (H. pylori) is a widespread human pathogen causing peptic ulcers and chronic gastritis. Maintaining nickel homeostasis is crucial for the establishment of H. pylori infection in humans. We used immobilized-nickel affinity chromatography to isolate Ni-related proteins from H. pylori cell extracts. Two-dimensional gel electrophoresis and mass spectrometry were employed to separate and identify twenty two Ni-interacting proteins in H. pylori. These Ni-interacting proteins can be classified into several general functional categories, including cellular processes (HspA, HspB, TsaA, and NapA), enzymes (Urease, Fumarase, GuaB, Cad, PPase, and DmpI), membrane-associated proteins (OM jhp1427 and HpaA), iron storage protein (Pfr), and hypothetical proteins (HP0271, HP jhp0216, HP jhp0301, HP0721, HP0614, and HP jhp0118). The implication of these proteins in nickel homeostasis is discussed. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Hindawi Publishing Corp. The Journal's web site is located at http://www.hindawi.com/journals/mbd/ | en_HK |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Chemistry | en_HK |
dc.subject | Analytical chemistry pharmacy and pharmacology | en_HK |
dc.title | Identification of proteins related to nickel homeostasis in Helicobater pylori by immobilized metal affinity chromatography and two-dimensional gel electrophoresis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0793-0291&volume=2008 Article ID 289490&spage=&epage=&date=2008&atitle=Identification+of+proteins+related+to+nickel+homeostasis+in+Helicobater+pylori+by+immobilized+metal+affinity+chromatography+and+two-dimensional+gel+electrophoresis | en_HK |
dc.identifier.email | Ge, R: geruig@hkusua.hku.hk | en_HK |
dc.identifier.email | Chiu, JF: jfchiu@hkucc.hku.hk | en_HK |
dc.identifier.email | Sun, H: sunhui116@hotmail.com | en_HK |
dc.identifier.email | He, QY: qyhe@hku.hk | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1155/2008/289490 | en_HK |
dc.identifier.pmid | 18288244 | en_HK |
dc.identifier.pmcid | PMC2225478 | en_HK |
dc.identifier.hkuros | 141636 | - |
dc.identifier.issnl | 0793-0291 | - |