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Article: Comparative techniques to quantify cyanobacteria dominated epilithic biofilms on tropical rocky shores

TitleComparative techniques to quantify cyanobacteria dominated epilithic biofilms on tropical rocky shores
Authors
KeywordsBiofilm
Cyanobacteria
Epilithic
Hong Kong
Tropical rocky shore
Issue Date1997
PublisherInter-Research. The Journal's web site is located at http://www.int-res.com/journals/meps/index.html
Citation
Marine Ecology Progress Series, 1997, v. 154, p. 281-291 How to Cite?
AbstractThe epilithic biofilm of tropical shores is dominated by cyanobacteria with only a sparse cover of diatoms. Geographical comparison of biofilms is difficult, however, due to variation in enumeration techniques. This paper describes techniques to quantify the biofilm in an attempt to standardize comparative studies. Species richness and relative abundance were best enumerated using scanning electron microscope (SEM) images of air dried rock chips, as compared to light, epifluorescence and confocal laser microscopy. SBM preparations provided clear images and allowed identification of most species. Air dried and Cryo-stage preparations provided the least damaged images of species whereas the harsh dehydration process in critical-point dried specimens damaged morphology and removed loosely attached species resulting in underestimation of species richness and abundance. A combination of SEM techniques (e.g. Cryo-stage, air dried and critical-point dried samples) is recommended for initial species identification. Chlorophyll a extraction from rock chips, as an indirect estimate of biomass, was more efficient using methanol or ethanol as compared to acetone. Methanol heated for 2 min and cooled for 12 h yielded 100% chlorophyll a extraction while cold methanol, methanol heated for 2 min and cooled for 3 and 6 h, and ethanol heated for 5 min, extracted greater than 95% chlorophyll a. No significant loss of chlorophyll a was recorded in rock samples stored in moist or dry conditions at 4°C or room temperature for 24 h or at -20°C for a month, but in samples stored at 4°C or room temperature for a week, 60 to 75% loss occurred. Chlorophyll a, once extracted, can be stored for a week in the dark prior to measurement without significant loss. To standardize techniques for tropical, cyanobacteria-rich epilithic biofilms, chlorophyll a extraction of rock chips using cold methanol is recommended.
Persistent Identifierhttp://hdl.handle.net/10722/57254
ISSN
2015 Impact Factor: 2.361
2015 SCImago Journal Rankings: 1.554
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNagarkar, Sen_HK
dc.contributor.authorWilliams, GAen_HK
dc.date.accessioned2010-04-12T01:30:59Z-
dc.date.available2010-04-12T01:30:59Z-
dc.date.issued1997en_HK
dc.identifier.citationMarine Ecology Progress Series, 1997, v. 154, p. 281-291en_HK
dc.identifier.issn0171-8630en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57254-
dc.description.abstractThe epilithic biofilm of tropical shores is dominated by cyanobacteria with only a sparse cover of diatoms. Geographical comparison of biofilms is difficult, however, due to variation in enumeration techniques. This paper describes techniques to quantify the biofilm in an attempt to standardize comparative studies. Species richness and relative abundance were best enumerated using scanning electron microscope (SEM) images of air dried rock chips, as compared to light, epifluorescence and confocal laser microscopy. SBM preparations provided clear images and allowed identification of most species. Air dried and Cryo-stage preparations provided the least damaged images of species whereas the harsh dehydration process in critical-point dried specimens damaged morphology and removed loosely attached species resulting in underestimation of species richness and abundance. A combination of SEM techniques (e.g. Cryo-stage, air dried and critical-point dried samples) is recommended for initial species identification. Chlorophyll a extraction from rock chips, as an indirect estimate of biomass, was more efficient using methanol or ethanol as compared to acetone. Methanol heated for 2 min and cooled for 12 h yielded 100% chlorophyll a extraction while cold methanol, methanol heated for 2 min and cooled for 3 and 6 h, and ethanol heated for 5 min, extracted greater than 95% chlorophyll a. No significant loss of chlorophyll a was recorded in rock samples stored in moist or dry conditions at 4°C or room temperature for 24 h or at -20°C for a month, but in samples stored at 4°C or room temperature for a week, 60 to 75% loss occurred. Chlorophyll a, once extracted, can be stored for a week in the dark prior to measurement without significant loss. To standardize techniques for tropical, cyanobacteria-rich epilithic biofilms, chlorophyll a extraction of rock chips using cold methanol is recommended.en_HK
dc.languageengen_HK
dc.publisherInter-Research. The Journal's web site is located at http://www.int-res.com/journals/meps/index.htmlen_HK
dc.relation.ispartofMarine Ecology Progress Seriesen_HK
dc.rightsMarine Ecology - Progress Series. Copyright © Inter-Research.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBiofilmen_HK
dc.subjectCyanobacteriaen_HK
dc.subjectEpilithicen_HK
dc.subjectHong Kongen_HK
dc.subjectTropical rocky shoreen_HK
dc.titleComparative techniques to quantify cyanobacteria dominated epilithic biofilms on tropical rocky shoresen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0171-8630&volume=154&spage=281&epage=291&date=1997&atitle=Comparative+techniques+to+quantify+cyanobacteria+dominated+epilithic+biofilms+on+tropical+rocky+shoresen_HK
dc.identifier.emailWilliams, GA: hrsbwga@hkucc.hku.hken_HK
dc.identifier.authorityWilliams, GA=rp00804en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.3354/meps154281en_HK
dc.identifier.scopuseid_2-s2.0-0030854937en_HK
dc.identifier.hkuros26826-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030854937&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume154en_HK
dc.identifier.spage281en_HK
dc.identifier.epage291en_HK
dc.identifier.isiWOS:A1997XR97600024-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridNagarkar, S=6602572935en_HK
dc.identifier.scopusauthoridWilliams, GA=7406082821en_HK

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