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Article: Pathway of phytate dephosphorylation by β-propeller phytases of different origins

TitlePathway of phytate dephosphorylation by β-propeller phytases of different origins
Authors
Keywordsβ-propeller phytase
Bacillus spp.
myo-inositol phosphate isomers
Phytate degradation
Shewanella oneidensis
Issue Date2007
PublisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjm
Citation
Canadian Journal Of Microbiology, 2007, v. 53 n. 4, p. 488-495 How to Cite?
AbstractUsing a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the P-propeller phytase of Shewanella oneidensis was established, which was then compared with that of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and B. amyloliquefaciens 45 β-propeller phytases. The data demonstrate that all of these β-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P 5, Ins(2,4,5,6)P 4 to finally Ins(2,4,6)P 3. Thus, the β-propeller phytases prefer the hydrolysis of every second phosphate over that of adjacent ones. This finding does not support previous phytate degradation models proposed by J. Kerovuo, J. Rouvinen, and F. Hatzack (2000. Biochem. J. 352: 623-628) and R. Greiner, A. Farouk, M. Larsson Alminger, and N.G. Carlsson (2002. Can. J. Microbiol. 48: 986-994), but seems to fit with the structural model given by S. Shin, N.C. Ha, B.C. Oh, T.K. Oh, and B.H. Oh (2001. Structure, 9: 851-858). © 2007 NRC.
Persistent Identifierhttp://hdl.handle.net/10722/57196
ISSN
2015 Impact Factor: 1.335
2015 SCImago Journal Rankings: 0.537
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGreiner, Ren_HK
dc.contributor.authorLim, BLen_HK
dc.contributor.authorCheng, Cen_HK
dc.contributor.authorCarlsson, NGen_HK
dc.date.accessioned2010-04-12T01:29:13Z-
dc.date.available2010-04-12T01:29:13Z-
dc.date.issued2007en_HK
dc.identifier.citationCanadian Journal Of Microbiology, 2007, v. 53 n. 4, p. 488-495en_HK
dc.identifier.issn0008-4166en_HK
dc.identifier.urihttp://hdl.handle.net/10722/57196-
dc.description.abstractUsing a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of myo-inositol hexakisphosphate dephosphorylation by the P-propeller phytase of Shewanella oneidensis was established, which was then compared with that of Bacillus subtilis 168, Bacillus amyloliquefaciens ATCC 15841, and B. amyloliquefaciens 45 β-propeller phytases. The data demonstrate that all of these β-propeller phytases dephosphorylate myo-inositol hexakisphosphate in a stereospecific way by sequential removal of phosphate groups via D-Ins(1,2,4,5,6)P 5, Ins(2,4,5,6)P 4 to finally Ins(2,4,6)P 3. Thus, the β-propeller phytases prefer the hydrolysis of every second phosphate over that of adjacent ones. This finding does not support previous phytate degradation models proposed by J. Kerovuo, J. Rouvinen, and F. Hatzack (2000. Biochem. J. 352: 623-628) and R. Greiner, A. Farouk, M. Larsson Alminger, and N.G. Carlsson (2002. Can. J. Microbiol. 48: 986-994), but seems to fit with the structural model given by S. Shin, N.C. Ha, B.C. Oh, T.K. Oh, and B.H. Oh (2001. Structure, 9: 851-858). © 2007 NRC.en_HK
dc.languageengen_HK
dc.publisherN R C Research Press. The Journal's web site is located at http://pubs.nrc-cnrc.gc.ca/cgi-bin/rp/rp2_desc_e?cjmen_HK
dc.relation.ispartofCanadian Journal of Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCanadian Journal of Microbiology. Copyright © N R C Research Press.en_HK
dc.rightsPublisher PDF may be archived 6 months after publicationen_HK
dc.subjectβ-propeller phytaseen_HK
dc.subjectBacillus spp.en_HK
dc.subjectmyo-inositol phosphate isomersen_HK
dc.subjectPhytate degradationen_HK
dc.subjectShewanella oneidensisen_HK
dc.titlePathway of phytate dephosphorylation by β-propeller phytases of different originsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-4166&volume=53&issue=4&spage=488&epage=495&date=2007&atitle=Pathway+of+phytate+dephosphorylation+by+β-propeller+phytases+of+different+originsen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1139/W07-015en_HK
dc.identifier.pmid17612603-
dc.identifier.scopuseid_2-s2.0-34347382434en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34347382434&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue4en_HK
dc.identifier.spage488en_HK
dc.identifier.epage495en_HK
dc.identifier.isiWOS:000247769300004-
dc.publisher.placeCanadaen_HK
dc.identifier.scopusauthoridGreiner, R=7102873664en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridCheng, C=7404797223en_HK
dc.identifier.scopusauthoridCarlsson, NG=7005250998en_HK

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