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Article: The human gC1qR/p32 gene, C1qBP. Genomic organization and promoter analysis

TitleThe human gC1qR/p32 gene, C1qBP. Genomic organization and promoter analysis
Authors
Issue Date2001
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2001, v. 276 n. 20, p. 17069-17075 How to Cite?
AbstractgC1qR is an ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, the 7.8-kilobase pair (kb) human gC1qPJp32 (C1qBP) gene was cloned and found to consist of 6 exons and 5 introns. Analysis of a 1.3-kb DNA fragment at the 5′-flanking region of this gene revealed the presence of multiple TATA, CCAAT, and Sp1 binding sites. Luciferase reporter assays performed in different human cell lines demonstrated that the reporter gene was ubiquitously driven by this 1.3-kb fragment. Subsequent 5′ and 3′ deletion of this fragment confined promoter elements to within 400 base pairs (bp) upstream of the translational start site. Because the removal of the 8-bp consensus TATATATA at -399 to -406 and CCAAT at -410 to -414 did not significantly affect the transcription efficiency of the promoter, GC-rich sequences between this TATA box and the translation start site may be very important for the promoter activity of the C1qBP gene. One of seven GC-rich sequences in this region binds specifically to PANC-1 nuclear extracts, and the transcription factor Sp1 was shown to bind to this GC-rich sequence by the supershift assay. Primer extension analysis mapped three major transcription start regions. The farthest transcription start site is 49 bp upstream of the ATG translation initiation codon and is in close proximity of the specific SP1 binding site.
Persistent Identifierhttp://hdl.handle.net/10722/54344
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTye, AJen_HK
dc.contributor.authorGhebrehiwet, Ben_HK
dc.contributor.authorGuo, Nen_HK
dc.contributor.authorSastry, KNen_HK
dc.contributor.authorChow, BKCen_HK
dc.contributor.authorPeerschke, EIBen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2009-04-03T07:43:57Z-
dc.date.available2009-04-03T07:43:57Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2001, v. 276 n. 20, p. 17069-17075en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54344-
dc.description.abstractgC1qR is an ubiquitously expressed cell protein that interacts with the globular heads of C1q (gC1q) and many other ligands. In this study, the 7.8-kilobase pair (kb) human gC1qPJp32 (C1qBP) gene was cloned and found to consist of 6 exons and 5 introns. Analysis of a 1.3-kb DNA fragment at the 5′-flanking region of this gene revealed the presence of multiple TATA, CCAAT, and Sp1 binding sites. Luciferase reporter assays performed in different human cell lines demonstrated that the reporter gene was ubiquitously driven by this 1.3-kb fragment. Subsequent 5′ and 3′ deletion of this fragment confined promoter elements to within 400 base pairs (bp) upstream of the translational start site. Because the removal of the 8-bp consensus TATATATA at -399 to -406 and CCAAT at -410 to -414 did not significantly affect the transcription efficiency of the promoter, GC-rich sequences between this TATA box and the translation start site may be very important for the promoter activity of the C1qBP gene. One of seven GC-rich sequences in this region binds specifically to PANC-1 nuclear extracts, and the transcription factor Sp1 was shown to bind to this GC-rich sequence by the supershift assay. Primer extension analysis mapped three major transcription start regions. The farthest transcription start site is 49 bp upstream of the ATG translation initiation codon and is in close proximity of the specific SP1 binding site.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntigens, CD44en_HK
dc.subject.meshPromoter Regions (Genetics)en_HK
dc.subject.meshProtein Biosynthesisen_HK
dc.subject.meshReceptors, Complement - chemistry - genetics - metabolismen_HK
dc.subject.meshRecombinant Proteins - metabolismen_HK
dc.titleThe human gC1qR/p32 gene, C1qBP. Genomic organization and promoter analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=276&issue=20&spage=17069&epage=17075&date=2001&atitle=The+human+gC1qR/p32+gene,+C1qBP.+Genomic+organization+and+promoter+analysisen_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1074/jbc.M009064200en_HK
dc.identifier.pmid11278463-
dc.identifier.scopuseid_2-s2.0-0035907281en_HK
dc.identifier.hkuros57444-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035907281&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume276en_HK
dc.identifier.issue20en_HK
dc.identifier.spage17069en_HK
dc.identifier.epage17075en_HK
dc.identifier.isiWOS:000168730400063-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTye, AJ=7003956414en_HK
dc.identifier.scopusauthoridGhebrehiwet, B=7005039582en_HK
dc.identifier.scopusauthoridGuo, N=7102597793en_HK
dc.identifier.scopusauthoridSastry, KN=24299502600en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.scopusauthoridPeerschke, EIB=7005991940en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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